Isolation, Identification and Antibiogram of Bacterial Pathogens from Bovine Subclinical Mastitis in Asella, Ethiopia

Lemu, Dereje; Bilata, Tsion

doi:10.5455/ijlr.20170524115206

Cited by 4 publications

(5 citation statements)

References 3 publications

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“…The current study showed that the overall prevalence of mastitis in lactating cows was 54.9% of which the clinical and subclinical mastitis accounted for 2.9% and 52.1%, respectively. This study finding is relatively comparable with various reports from different parts of the country (Sori et al, 2005;Lakew et al, 2009;Abunna et al, 2013;Teklesilasie et al, 2014;Pal et al, 2017;Abebe et al, 2020). Subclinical mastitis was more prevalent (54.9% and 95% CI= 49.9-59.9) than clinical mastitis (2.9% and 95% CI= 1.6 -5.1) in the study area, which is in agreement with various studies reported from various parts of the country (Getaneh and Gebremedhin, 2017;Abebe et al, 2020;Fesseha et al, 2021;Belay et al, 2022;Demil et al, 2022;Girma and Tamir, 2022).…”

Section: Discussionsupporting

confidence: 92%

Bovine mastitis: Prevalence, causes and associated risk factors in Silte Zone, Ethiopia

Tesfay,

Abda,

Sheferaw

2023

Ethiop. Vet. J.

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Mastitis is an important disease of dairy cows, and it causes huge economic losses to dairy farm owners due to a decrease in milk production, and reduction in milk quality, and an increase in the cost of cow treatment. A crosssectional study was conducted in Southern Ethiopia’s Silte zone from October 2020 to June 2021 aimed to estimate mastitis prevalence, assess related risk factors, and identify prevalent bacterial causes. Three hundred eighty-four lactating cows were examined for abnormalities in udder quarters and teats. Milk samples were tested for subclinical mastitis via the California mastitis test (CMT) and cultured for causative agents from clinical mastitic and CMT-positive cows. The overall prevalence of mastitis was 54.9% (95% CI=49.9-59.9), of which 52.1% (95% CI=47.1-57.1) was subclinical and 2.9% (95% CI=1.6 -5.1) clinical mastitis. From a total of 1536 quarters examined 41 (2.7%) quarters were found blind and 427 (27.7%) quarters were affected by mastitis. Overall, more hind quarters, 236 (55.5%) were affected than the front quarters, 189 (44.5%) of udder. The prevalence of mastitis was significantly higher during early lactation, ≤4 months (p< 0.05); and it was increased with increasing parity (p< 0.05) and age (p< 0.05). Cows with no bedding were more affected than those with bedding (p< 0.05). Multivariable logistic regression showed that cows with round and flat teat ends were 2.84 and 11.85 times more likely to contract mastitis. Also, cows with pendulous udder, producing more than 10 liters per day milk and during the early 4 months of lactation were 1.87, 6.81, and 2.14 more likely affected by mastitis than normal udder, producing less than 10 liters of milk per day and lactation after five months, respectively. Milk samples collected from 211 mastitis-positive cows were cultured using standard bacteriological technique, and the isolated bacteria were Staphylococcus aureus (29.5%), Staphylococcus epidermidis (14.2%), Staphylococcus intermedius (11.6%), Staphylococcus hyicus (11.1%), Streptococcus agalactiae (8.9%), Streptococcus disgalactiae (6.3%), E. coli (5.8%), Streptococcus uberis(5.3%), Klebsiella spp. (1.6%) and Enterococcus spp. (1.1%). Owners should givepro per udder care and are advised to apply dry cow therapy. Extension workers should raise awareness.

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Section: Discussionsupporting

confidence: 92%

Bovine mastitis: Prevalence, causes and associated risk factors in Silte Zone, Ethiopia

Tesfay,

Abda,

Sheferaw

2023

Ethiop. Vet. J.

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“…The results were comparable to the ones found in Ethiopia [21] which found a prevalence of 61.1%. However, the results were lower than those reported in Nigeria (85.5%) [22], Ethiopia (72.7%) [23]. The reason for high SCM prevalence in the present study may be due to lack of proper mastitis control practices in the study area.…”

Section: Discussioncontrasting

confidence: 88%

Occurrence of Antibiotic Resistance in Bacteria Isolated from Milk of Dairy Cows in Small-Holder Farms in Juja Sub-County, Kenya

Kagira,

Ngotho,

Mugo

et al. 2022

AJRAVS

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Sub-clinical mastitis (SCM) remains a serious hindrance to small-holder dairy farms in Kenya. A cross-sectional study was conducted to determine the risk factors associated with occurrence of SCM and antibiotic resistance in smallholder dairy farms in a peri-urban set-up area of Juja Subcounty, Kenya. Milk samples was obtained from 120 lactating dairy cows in 60 farms and screened for SCM using California mastitis test (CMT). Positive CMT samples were cultured and bacteria identification was done using standard methods. A questionnaire survey was administered at the household level to assess the risk factors associated with SCM. Sensitivity of the Staphylococcus aureus and Escherichia coli isolated from milk against commonly used antibiotics was determined using disc diffusion method. The prevalence of SCM at cow and udder level was found to be 66.7% and 61.3%, respectively. The highest prevalence of SCM was in Kalimoni (88.8%), Murera (82.7%) and Witeithe (71.4%) wards. The least affected area being Juja (60%) and Theta (44.4%) wards. Sub-clinical mastitis was significantly higher (p=0.007) in Friesian (74.1%) and Guernsey (66.7%) breeds as compared to the indigenous breed (53.8%). Cows with parity of four and above had higher (p=0.001) prevalence of SCM (73.7%) as compared to those of lower parity stage.

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“…The mean sample size of the 46 studies was 343 ± 165.4 cows. Among the 46 studies, three studies focused on estimating the prevalence and isolation of pathogens related to subclinical mastitis whereas the remaining studies included both types of bovine mastitis ( Pal, Lemu & Bilata, 2017 ; Mekonnen et al, 2017 ; Tegegne et al, 2020 ). Except for two studies, all studies performed a physical examination of the mammary gland and subsequent collection of milk samples for analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Except for two studies, all studies performed a physical examination of the mammary gland and subsequent collection of milk samples for analysis. Likewise, they have performed the California mastitis test and applied standard microbiological culturing techniques for the isolation of potential bacterial agents ( Pal, Lemu & Bilata, 2017 )( Birhanu et al, 2017 ). Concerning the regional states of study in the Ethiopia, 21 studies were from Oromia, 10 studies from SNNPR, five studies from Addis Ababa, four studies from Amhara, two studies from Somali, two studies from Dire Dawa and two studies from Tigray regional states ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Bacterial profile of bovine mastitis in Ethiopia: a systematic review and meta-analysis

Tora

Belay²,

Kumar

2022

PeerJ

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Background Bovine mastitis is the commonest episode of infection in the dairy industry, which often occurs after damage of epithelial cells lining the teat duct. It is ranked as the second most important cause of milk production loss directly and a devastating disease with a higher incidence leading to the culling of dairy cows. Thus, this systematic review and meta-analysis is aimed to quantitatively estimate the current status of mastitis in general and bacterial mastitis particular in Ethiopia. Methods A literature search was carried from major databases and indexing services including PubMed, Google Scholar, Science Direct. Also, local institution repositories were searched to retrieve unpublished MSc and PhD theses. All studies were included addressing the prevalence of mastitis and bacterial isolates conducted in Ethiopia. Microsoft Excel was used to extract data and was imported to R Studio for the analyses. The random-effects model at a 95% confidence level was used for pooled estimates of outcomes. The degree of heterogeneity was computed by Higgins’s I2 statistics. Publication bias was checked by using the funnel plots of standard error augmented by Begg’s and Egger’s tests. Results A total of 46 studies with 15,780 cows were included in this study. All studies have collected 18,478 suspected samples for bacterial isolation. While pooled prevalence estimate of mastitis was 47.6%, the bacterial isolates pooled prevalence was 33.1%. The bacterial mastitis was 6.5% in cows infected by clinical patients and was 28.3% subclinical patients. The common isolates were Staph aureus, Strep species, Staph epidermis, Escherchia coli, Corynebacterium bovis, Coagulase Negative Staphylococcus and Klebsiella species. A univariate meta-regression analysis evidenced that the type of mastitis and management system was a possible source of heterogeneity (P-value = 0.001). Conclusion The pooled prevalence of bacterial mastitis in Ethiopian dairy cattle was high. The analysis showed bacterial pathogens like Staphylococcus aureus, Staph epidermis, Strep species and Coagulase Negative Staphylococcus are majorly accounted for bovine mastitis in Ethiopia. Therefore, the highly prevalent and commonly isolated pathogens cause contagious mastitis which require immediate attention by dairy producers to put under control by devising robust mastitis prevention and control interventions.

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Isolation, Identification and Antibiogram of Bacterial Pathogens from Bovine Subclinical Mastitis in Asella, Ethiopia

Cited by 4 publications

References 3 publications

Bovine mastitis: Prevalence, causes and associated risk factors in Silte Zone, Ethiopia

Bovine mastitis: Prevalence, causes and associated risk factors in Silte Zone, Ethiopia

Occurrence of Antibiotic Resistance in Bacteria Isolated from Milk of Dairy Cows in Small-Holder Farms in Juja Sub-County, Kenya

Bacterial profile of bovine mastitis in Ethiopia: a systematic review and meta-analysis

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