Isolation, Cloning and Determination of Biologic Characteristics of Five New Species of Crithidia

McGhee, Robert B.; Hanson, William L.; Schmittner, Stella M.

doi:10.1111/j.1550-7408.1969.tb02310.x

Cited by 21 publications

(12 citation statements)

References 11 publications

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“…Isolated round forms with a definite cystic wall were seldom seen. However, the majority of the free epimastigotes presented buds along the flagellum, of the type called straphangers, thought to represent precystic forms (7,12).…”

Section: Natural Infection Of the Insect Hostmentioning

confidence: 99%

Trypanosomatidae Isolated from Zelus leucogrammus (Perty, 1834) (Hemiptera, Reduviidae), with a Discussion on Flagellates of Insectivorous Bugs*

Carvalho

Deane

1974

The Journal of Protozoology

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SYNOPSIS. The insectivorous bug Zelus leucogrammus (Perty, 1834) (Hemiptera, Reduviidae) was found to harbor several species of trypanosomatids of the genera Leptomonas, Crithidia, Herpetomonas and Blastocrithidia. However, laboratory bred nymphs given sterile food were infected only by Blastocrithidia, the other flagellates appearing in their intestinal tract when they were fed on other insects.The Blastocrithidia was thought to belong to a single species and to be a parasite proper of Z . leucogrammus (and possibly other Hemiptera) , producing luxuriant infections in the intestinal tract of the bugs captured in nature and spreading easily among isolated populations through cannibalism and ingestion of cysts; it was also abundant in the genital tract of gravid females but transovarian transmission was not proved. This Blastocrithidia experimentally infected Triatoma infestans and is very close to B. triatomae Cerisola, del Prado, Rohwedder and Bozzini, 1971. The other trypanosomatids, never numerous in naturally infected Z . leucogrammus, are acquired by the bug from many different insects, chiefly Diptera, on which it was seen to feed. Herpetomonas muscarum and C . luciliae, the common trypanosomatids among several muscoid Diptera, were isolated both from flies and from the bugs fed on them.

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Section: Natural Infection Of the Insect Hostmentioning

confidence: 99%

Trypanosomatidae Isolated from Zelus leucogrammus (Perty, 1834) (Hemiptera, Reduviidae), with a Discussion on Flagellates of Insectivorous Bugs*

Carvalho

Deane

1974

The Journal of Protozoology

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“…ONCOPELTUS fasciatus is a natural host of several species of lower trypanosomatids belonging to the genera Crithidia (Hanson and McGhee 1961; McGhee, Hanson, and Schmittner 1969), Leptomonas (McGhee and Hanson 1962; Noguchi and Tilden 1926), and Phytomonas (Holmes 1925; McGhee and Hanson 1964). Infected loci in Crithidia acanthocephali (Hanson and McGhee 1961) or Leptomonas oncopelti (Laugé and Nishioka 1977; McGhee and Hanson 1962) are in the intestinal tract, and in Phytomonas elmassiani (McGhee and Hanson 1964) in salivary glands and hemolymph.…”

mentioning

confidence: 99%

Ultrastructural and Biochemical Characterization of Promastigote and Cystic Forms of Leptomonas wallacei N. Sp. Isolated from the Intestine of its Natural Host Oncopeltus fasciatus (Hemiptera: Lygaeidae)

Romeiro

Solé‐Cava

Sousa

et al. 2000

J Eukaryotic Microbiology

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Promastigote forms of a trypanosomatid were isolated from the third and fourth ventricles of the midgut and from the hindgut of the phytophagous hemipteran Oncopeltus fasciatus. Some individuals had adhered to its anterior region, close to the flagellar pocket, or to the flagellum up to four rounded aflagellated forms known as straphangers cysts. Scanning electron microscopy revealed that the flagellated forms presented a twisted cell body and a long flagellum, and the cysts, smaller than the parental promastigote, had a nascent flagellum. Transmission electron microscopy showed that promastigotes were typical, while cystic forms were ovoid dense cells devoid of a cyst wall, but presenting a cell coat, a special subpellicular region limited by a membrane unit, and a condensed cytoplasm. The kinetoplast-DNA fibrils appeared as dense spots and the condensed chromatin was arranged in a labyrinthic structure. Desmosome-like structures, observed in the region of adhesion of the precystic forms to the parental promastigote, could explain how cysts remain attached to the mother cell during the encystation process. Release of membranes from the surface of promastigotes and cysts seems to be correlated with the condensation of the cytoplasm during encystment. Morphological and isozyme analyses indicated that this trypanosomatid belongs to the genus Leptomonas. The molecular karyotype of this isolate was compared with that of a strain of Leptomonas oncopelti obtained from Oncopeltus varicolor by contour-clamped homogeneous electric field (CHEF) electrophoresis and revealed similar DNA banding patterns between 2,200-825 Kb, but not in lower bands (825-225 Kb). This suggested that the isolate from O. fasciatus and that from O. varicolor were not identical. Based on our findings we are describing Leptomonas wallacei n. sp. for our isolate from O. fasciatus.

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“…The range and optimum temperature, pH, and osmolarity are important criteria. A number of thermophilic forms that grow at 37°C (40) and some that require or tolerate a low pH (29) have been described. Although colony characteristics of trypanosomatids grown on agar plates have not been used i n any species descriptions.…”

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confidence: 99%

Guidelines for the Description of New Species of Lower Trypanosomatids1

Wallace¹,

Camargo

McGhee

et al. 1983

The Journal of Protozoology

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Morphological, cultural, and biochemical criteria that have been used in describing lower trypanosomatids, genera Blastocrithidia, Crithidia, Leptomonas, Herpetomonas, Rhynchoidomonas, and Phytomonas are reviewed. Kinetoplast structure, carbohydrate utilization, electrophoretic mobilities of isoenzymes, and kDNA fingerprinting are among the recommended criteria for species differentiation. Temperature, pH, and osmolarity tolerance are useful growth parameters. Generic placement may be assisted by the determination of nitrogenous excretion products and ornithine‐arginine cycle enzymes.

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Isolation, Cloning and Determination of Biologic Characteristics of Five New Species of Crithidia

Cited by 21 publications

References 11 publications

Trypanosomatidae Isolated from Zelus leucogrammus (Perty, 1834) (Hemiptera, Reduviidae), with a Discussion on Flagellates of Insectivorous Bugs*

Trypanosomatidae Isolated from Zelus leucogrammus (Perty, 1834) (Hemiptera, Reduviidae), with a Discussion on Flagellates of Insectivorous Bugs*

Ultrastructural and Biochemical Characterization of Promastigote and Cystic Forms of Leptomonas wallacei N. Sp. Isolated from the Intestine of its Natural Host Oncopeltus fasciatus (Hemiptera: Lygaeidae)

Guidelines for the Description of New Species of Lower Trypanosomatids1

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