Isolation and Screening of Thermo stable Extracellular Alkaline Protease Producing Bacteria from Tannery Effluents

Habib, Shazia; Fakhruddin, A. N. M.; Begum, Shamima; Ahmed, Monzur Morshed

doi:10.3329/jsr.v4i2.6976

Cited by 8 publications

(3 citation statements)

References 12 publications

(13 reference statements)

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“…1b). Comparable reports about the isolation of protease producing bacteria utilizing nutrient with skim milk medium and/or minimum medium supplemented with skim milk have been documented [24,25]. Based on the morphological characteristics and Gram stain, the bacterial isolate was classified as Gram-positive rod-shaped cells.…”

Section: Bacterial Isolation and Identificationmentioning

confidence: 99%

Whey Fermentation for Protease Production Using <i>Bacillus thuringiensis</i> Isolated from Mangrove Rhizosphere Soil in Jazan, Saudi Arabia

El-Gayar¹,

Essa²,

Abada³

2020

Pol. J. Environ. Stud.

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Industrial wastes are considered a real source of environmental pollution and health hazards since various types of microorganisms, including pathogens, have the ability to grow on them [1]. Food industries are characterized by high water consumption and production of huge amounts of effluents and sludge per unit of production [2]. Milk contains nutritive compounds and biologically active ones such as caseins and whey proteins. Caseins constitute 80% of the total protein present in milk. Meanwhile, the remaining 20% is comprised of α-lactalbumin, β-lactoglobulin, and immunoglobulins [1, 3]. The annual production of whey

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Section: Bacterial Isolation and Identificationmentioning

confidence: 99%

Whey Fermentation for Protease Production Using <i>Bacillus thuringiensis</i> Isolated from Mangrove Rhizosphere Soil in Jazan, Saudi Arabia

El-Gayar¹,

Essa²,

Abada³

2020

Pol. J. Environ. Stud.

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“…Alkaline proteases, which exhibit maximum activity at pH values of 8 and above, are produced by several microorganisms, including bacteria . Alkalophilic proteases, in particular, have demonstrated significant utility in the detergent industry, while acidophilic proteases have found applications in leather tanning, food processing, and x-ray film production (Habib et al, 2012;Rayda et al, 2012;Badgujar & Mahajan, 2013). The genus Bacillus is vital for commercially important alkaline protease (EC.…”

Section: Introductionmentioning

confidence: 99%

Screening and isolation of protease-producing bacteria from wastewater samples in Obafemi Awolowo University (OAU) Campus, Ile-Ife, Nigeria

Qudus,

Abiodun

2023

Preprint

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Background: Wastewater samples possess substantial potential as a valuable resource for the isolation of bacteria with the capacity to produce protease enzymes. Gaining insights into the proteolytic capabilities of these bacteria holds considerable significance for a wide range of industrial applications. Enhancing our understanding of the microbial diversity and protease production potential within wastewater can pave the way for the creation of customized enzymatic solutions tailored specifically for industrial needs. Purpose: The purpose of this study was to isolate and identify protease-producing bacteria from wastewater samples collected at Obafemi Awolowo University Campus in Nigeria. The study involved isolating bacteria from the wastewater, identifying them, evaluating their growth on protease-supporting agar, determining their proteolytic activities, and screening bacterial colonies for protease production using skim milk agar medium. Methods: Wastewater samples were aseptically collected from various locations within Obafemi Awolowo University Campus, located in Ile-Ife, Osun State, Nigeria. Bacterial isolation from the wastewater was performed using the serial dilution technique. The samples were progressively diluted and plated onto nutrient agar media for bacterial growth. Skim milk agar media was specifically used to isolate protease-producing bacteria. Following the isolation, screening was conducted to identify potential protease-producing bacteria. Zonal inhibition methods were employed using skim milk agar media during the screening process. The objective was to select bacterial isolates that exhibited clear zones around their colonies, indicating protease activity. To identify the potential protease-producing bacteria, morphological and biochemical tests were conducted. These tests included observations of colonial morphology, cellular morphology, and biochemical characteristics. The Bergey's Manual was used as a reliable reference for taxonomic classification during the identification process. Results: Among the ten bacterial colonies obtained from the wastewater samples, eight exhibited clear zones, indicating protease activity. Morphological and biochemical tests identified the protease-producing bacteria as Bacillus spp. and Pseudomonas spp. Further characterization revealed that the Bacillus licheniformis isolate from Water Sample D1 (WSD1) displayed the highest protease activity. Bacillus subtilis isolates also showed significant protease production, while Pseudomonas spp. exhibited lower protease production. Conclusions: Wastewater samples from the OAU Campus yielded protease-producing bacteria, with Bacillus licheniformis showing the highest activity. The findings highlight the industrial potential of the isolated Bacillus licheniformis strain and emphasize the significance of utilizing wastewater as a source for obtaining bacteria with protease production capabilities. Further studies on individual strains within the Bacillus and Pseudomonas genera may lead to the discovery of strains with enhanced protease production, enabling tailored enzymatic solutions for various industrial sectors. Overall, this study contributes to our understanding of protease-producing microorganisms.

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“…There is renewed interest in the study of proteolytic enzymes, mainly due to the recognition that these enzymes not only play an important role in the cellular metabolic processes but have also gained considerable attention in the industrial process (Gupta et al, 2005;Habib et al, 2012). Proteases represents one of the three largest groups of industrial enzymes and have traditionally held the predominant share of the industrial enzyme market accounting for about 60% of total sale of enzymes (Beg et al, 2003;Deng et al, 2010) Protease production is an inherent capacity of some microorganisms and large numbers of bacterial species are known to produce alkaline proteases.…”

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confidence: 99%

Isolation and classical identification of potent extracellular alkaline protease producing alkalophilic Bacillus sp from coastal regions of Tamil Nadu

Saminathan¹,

Narayanan²

2015

Afr. J. Microbiol. Res.

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Isolation and Screening of Thermo stable Extracellular Alkaline Protease Producing Bacteria from Tannery Effluents

Cited by 8 publications

References 12 publications

Whey Fermentation for Protease Production Using <i>Bacillus thuringiensis</i> Isolated from Mangrove Rhizosphere Soil in Jazan, Saudi Arabia

Whey Fermentation for Protease Production Using <i>Bacillus thuringiensis</i> Isolated from Mangrove Rhizosphere Soil in Jazan, Saudi Arabia

Screening and isolation of protease-producing bacteria from wastewater samples in Obafemi Awolowo University (OAU) Campus, Ile-Ife, Nigeria

Isolation and classical identification of potent extracellular alkaline protease producing alkalophilic Bacillus sp from coastal regions of Tamil Nadu

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