Isolation and purification of bacterial poly(3-hydroxyalkanoates)

Jacquel, Nicolas; Lo, Chieh-Pu; Wei, Yuhong; Wu, Ho‐Shing; Wang, Shaw S.

doi:10.1016/j.bej.2007.11.029

Cited by 343 publications

(202 citation statements)

References 58 publications

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“…In the first case the goal is to enhance the productivity while choosing low cost fermentation substrates [4,8], in the second case the goal is to improve the technology of extraction, separation and purification of the produced polymer [9][10]. In particular, the downstream alone is responsible for 60-80% of the total cost [11]. Even though the price of poly-3-hydroxybutyrate lowered from ca.…”

Section: Introductionmentioning

confidence: 99%

“…The most routinely used P(3HB) solvents in laboratory scale are chlorinated organic compounds, namely chloroform and dichloromethane [10,17].However, large-scale application of these solvents causes environmental and health concern. Moreover, the process requires high volumes of solvent and anti-solvent for the precipitation [11], increasing the cost of extraction and subsequent solvent recovery. Some efforts have been done towards the selection of green solvents [18][19], although the definition of "green solvents" by itself is not simple and is a subject of study [20].…”

Section: Introductionmentioning

confidence: 99%

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Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Rosengart

Cesário

Almeida

et al. 2015

Biochemical Engineering Journal

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A technique for poly-3-hydroxybutyrate (P(3HB)) extraction with safer, non-chlorinated solvents, was developed, aiming to attain high recovery yields and purities. A wide range of solvents was selected from the GlaxoSmithKline guide as sustainable industrial solvents and the solubility of P(3HB) on those solvents calculated using predictive equations from literature. Anisole, * Corresponding author: Tel.: +351 21 8419137; Fax: +351 21 8419062. e-mail address: teresa.cesario@tecnico.ulisboa.pt + Current address: Politecnico di Milano, Dipartimento di Chimica Materiali e Ingegneria Chimica "Giulio Natta". Via Mancinelli, 7 -20131 Milano MI 2 cyclohexanone and phenetole were used as extraction solvents and the relevant process variables (extraction temperature, extraction time and mass of cells/solvent volume ratio) were optimized.Polymer recovery yields of 97% and 93% were obtained with anisole and cyclohexanone, respectively, at 120-130°C using a cell/solvent ratio of 1.5% (w/v). Maximum polymer purities using these experimental conditions were 98% for both solvents. Recovery yields and polymer purity attained with chloroform (reference solvent) were 97 and 98%, respectively. Higher cell/solvent ratios of 6.0 % (w/v) showed slightly lower recovery yields and purities The average molecular weight and the thermal properties of the polymers extracted with the alternative solvents were comparable to those of the polymers obtained by chloroform extraction, showing that the applied conditions did not significantly alter the properties of the extracted P(3HB).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Rosengart

Cesário

Almeida

et al. 2015

Biochemical Engineering Journal

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“…In particular, the possibility to adjust material properties by controlling the monomeric composition, and functionalize it via introduction of vinyl groups is of great interest 1 . Whereas short-chain-length PHAs (scl-PHA, C4-C5 monomers) are readily available on the market 2,3 , largescale commercialization of mcl-PHA is still to be established. Reasons for this are lower PHA yields, more costly substrates (e.g.…”

Section: Introductionmentioning

confidence: 99%

Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

Follonier

Riesen²,

Zinn

2015

Chem.Biochem.Eng.Q.

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“…The PHA concentration was determined by gas chromatography (GC) as reported previously [18][19][20]. Cells cultured for 48 h in N-, K-and S-limited minimal medium were harvested by centrifugation at 4,000×g for 10 min, and washed three times with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Production of Polyhydroxybutyrate from Crude Glycerol and Spent Coffee Grounds Extract by Bacillus cereus Isolated from Sewage Treatment Plant

Lee¹,

Choi²,

Na³

et al. 2014

KSBB Journal

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Production of biodegradable polymer polyhydroxyalkanoates (PHAs) from industrial wastes exhibits several advantages such as recycle of waste and the production of high valuable products. To this end, this study aimed at isolating from the sewage treatment plant a PHA producing bacterium capable of utilizing wastes generated from biodiesel and food industries. A Bacillus cereus strain capable of producing poly(3-hydroxybutyrate) [P(3HB)] was isolated, which was followed by confirmation of P(3HB) accumulation by gaschromatographic analyses. Then, the effects of nutrient limitation on P(3HB) production by B. cereus was first examined. Cells cultured in a minimal medium under the limitation of nitrogen, potassium and sulfur suggested that nitrogen limitation allows the highest P(3HB) accumulation. Next, production of P(3HB) was examined from both waste of biodiesel production (crude glycerol) and waste from food industry (spent coffee grounds). Cells cultured in nitrogen-limited minimal medium supplemented crude glycerol and waste spent coffee grounds extract accumulated P(3HB) to the contents of 2.4% and 1.0% of DCW. This is the first report demonstrating the capability of B. cereus to produce P(3HB) from waste raw materials such as crude glycerol and spent coffee grounds.

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Isolation and purification of bacterial poly(3-hydroxyalkanoates)

Cited by 343 publications

References 58 publications

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

Production of Polyhydroxybutyrate from Crude Glycerol and Spent Coffee Grounds Extract by Bacillus cereus Isolated from Sewage Treatment Plant

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