Isolation and propagation of phages naturally associated with the aizawai variety of <i>Bacillus thuringiensis</i>

Inal, Jameel M.; Karunakaran, V.; Burges, H. D.

doi:10.1111/j.1365-2672.1990.tb02543.x

Cited by 13 publications

(12 citation statements)

References 8 publications

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“…Other probably similar phage, φHD248, is described above. Generalized transduction was demonstrated for the φHD248, which confirms that such phages can be useful for limited spectra of hosts (Inal et al 1990(Inal et al , 1992(Inal et al , 1996.…”

Section: Potentially Transducing Phages For the B Cereus Groupsupporting

confidence: 75%

“…The temperate phages φHD67, φHD130, φHD228 and φHD248 isolated after UV-induction from the subspecies aizawai (Inal et al 1990) have been shown to mediate both intra-subspecific ( aizawai) and inter-subspecific ( aizawai-kurstaki) generalized transduction (Inal et al 1992). The phage ϕHD248 was used for genetic analysis of B. thuringiensis subsp.…”

Section: Gene Transduction Experimentsmentioning

confidence: 99%

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Bacillus thuringiensis Genetics and Phages—From Transduction and Sequencing to Recombineering

Sorokin

2012

Bacillus Thuringiensis Biotechnology

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Experimental mapping of genes was less intensive in the Bacillus cereus group, which includes B. thuringiensis, than in Bacillus subtilis, generally considered as the model for Gram-positive bacteria. Nevertheless the genomic sequencing equalized densities of available gene maps. Moreover, the genes responsible for such complex phenomena like virulence or psychrotolerance could only be identified using the authentic bacteria prone to possess these properties. The new experimental approaches of post-genomic genetics should therefore be considered. Phage-mediated gene transduction and recombineering perspectives for the B. cereus group are reviewed. In combination with new generation sequencing these approaches will constitute the gene identification methodologies in the post-genomics time.

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Section: Potentially Transducing Phages For the B Cereus Groupsupporting

confidence: 75%

Section: Gene Transduction Experimentsmentioning

confidence: 99%

Bacillus thuringiensis Genetics and Phages—From Transduction and Sequencing to Recombineering

Sorokin

2012

Bacillus Thuringiensis Biotechnology

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“…This generates nicks in supercoiled DNA (Gonzalez & Carlton, 1980) but it did not alter the migration of the #HD248 DNA, unlike that of the control ligated 3, DNA, for which a decrease in the amount of circular DNA was observed (Inal, 1988). t Bands A1 and C1 are minor bands present in submolar amounts (see text).…”

Section: Hd248 Dna Lacks Cohesive Endsmentioning

confidence: 99%

Bacillus thuringiensis subsp. aizawai generalized transducing phage 4HD248 : restriction site map and potential for fine-structure chromosomal mapping

1996

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The temperate, generalized transducing phage 4HD248 from Bacillus thuringiensis subsp. aizawai (H-serotype 7) was shown previously to mediate transduction within subsp. aizawai and between subsp. aizawai and subsp. kurstaki (H-serotype 3a3b). Here it was found to have a broad host range, plating on 7 out of 14 H-serotypes tested. Cotransduction analysis using 4HD248 revealed two linkage groups in H-serotype 7 : bio-24is-2-met-l-cit-1-a~-2-lys-3 and trp-9-purCl-thi-I. Preliminary characterization of the 4HD248 genome revealed a linear double-stranded DNA of 47.15 kb. A restriction site map is reported including a total of 10 sites for the enzymes BamHI, Bg/ll, Sall and Sstl. Enzyme Sah was found to have a unique cleavage site which if not located in an essential region could be used as a cloning site. The 4HD248 genome was also shown to lack cohesive ends and appears to have a partial circular permutation.

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“…UV light can induce bacteriophage production in B. thuringiensis subsp. aizawai (14) and in B. thuringiensis subsp. kurstaki HD-1 as measured by lysis of B. cereus 569 (A. Aronson, unpublished results), so a major reason for the extreme sensitivity of these plasmidbearing strains could be phage induction.…”

Section: Resultsmentioning

confidence: 99%

Plasmid-associated sensitivity of Bacillus thuringiensis to UV light

Benoit¹,

Wilson²,

Bull³

et al. 1990

Appl Environ Microbiol

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Spores and vegetative cells of BaciUus thuringiensis were more sensitive to UV light than were spores or cells of plasmid-cured B. thuringiensis strains or of the closely related Bacillus cereus. Introduction of B. thuringiensis plasmids into B. cereus by cell mating increased the UV sensitivity of the cells and spores. Protoxins encoded by one or more B. thuringiensis plasmids were not involved in spore sensitivity, since a B. thuringiensis strain conditional for protoxin accumulation was equally sensitive at the permissive and nonpermissive temperatures. In addition, introduction of either a cloned protoxin gene, the cloning vector, or another plasmid not containing a protoxin gene into a plasmid-cured strain of B. thuringiensis all increased the UV sensitivity of the spores. Although the variety of small, acid-soluble proteins was the same in the spores of all strains examined, the quantity of dipicolinic acid was about twice as high in the plasmid-containing strains, and this may account for the differences in UV sensitivity of the spores. The cells of some strains harboring only B. thuringiensis plasmids were much more sensitive than cells of any of the other strains, and the differences were much greater than observed with spores.

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Isolation and propagation of phages naturally associated with the aizawai variety of Bacillus thuringiensis

Cited by 13 publications

References 8 publications

Bacillus thuringiensis Genetics and Phages—From Transduction and Sequencing to Recombineering

Bacillus thuringiensis Genetics and Phages—From Transduction and Sequencing to Recombineering

Bacillus thuringiensis subsp. aizawai generalized transducing phage 4HD248 : restriction site map and potential for fine-structure chromosomal mapping

Plasmid-associated sensitivity of Bacillus thuringiensis to UV light

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