Isolation and phenotypic characterization of actinomycetes from Rabat neighborhood soil and their potential to produce bioactive compounds

Hasnaa, Ayoubi; Assia, Mouslim; Sara, Moujabbir; Soukaina, Amine; Iman, Azougar; Mouslim, Jamal; Menggad, Mohammed

doi:10.5897/ajmr2017.8761

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…The orange color was shown in the ISP2 liquid medium because the cell biomass was still mixed with the medium. Previous reports said that ISP2 is a medium that can be used for pigment production in actinomycetes (Ratte et al 2022) and is the best media for higher pigment production (Ayoubi et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of an Actinomycete Gordonia terrae JSN1.9-Derived Orange Pigment Extract

Sholekha,

Budiarti,

Hasan

et al. 2023

HAYATI J Biosci

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Actinomycetes are known to be a source of natural products and drugs. Gordonia terrae, an actinomycete pigment producer, shows potential in producing pigment with antimicrobial activity. This study aims to determine the antimicrobial activity of the active pigment fraction produced by the actinomycete G. terrae, assess the effects of the active pigment fraction on microbial cells, and identify the types of compounds present in the fraction. The pigment extract exhibited antimicrobial activity against Gram-positive bacteria and fungi. Specifically, it showed activity against Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25293, and Candida albicans ATCC 10231. Furthermore, the study evaluated the antimicrobial activities of the active fractions, revealed the active fraction had better antimicrobial activity than the crude extract. Scanning electron microscopy (SEM) confirmed that the active fraction of the pigment causes damage to B. subtilis ATCC 6633 cells, inhibits the formation of filaments in C. albicans ATCC 10231, and alters the normal shape of the cells. LC-MS/MS results showed that the active fraction contained several compounds known for their antimicrobial activity. Among the dominant compounds identified were cholestyramine, aminopregnane, and sphinganine. Thus, this study demonstrated that the orange pigment extract derived from G. terrae JSN1.9 exhibits promising antimicrobial activity.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of an Actinomycete Gordonia terrae JSN1.9-Derived Orange Pigment Extract

Sholekha,

Budiarti,

Hasan

et al. 2023

HAYATI J Biosci

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“…Actinomycetes play a major role in combating various diseases. Earlier exploration has shown actinomycetes having excellent potential to control the growth of pathogens (Ayoubi et al 2018). In our study, a total of 240 endophytic actinomycetes were isolated and screened for their antimicrobial activities, out of which 77 actinomycetes showed the antimicrobial property.…”

Section: Discussionmentioning

confidence: 99%

Extraction and Characterization of Antibiotic Compounds produced by Streptomyces spp. VITGV01 against Selected Human Pathogens

Veilumuthu

Siva

Christopher

2021

Preprint

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Streptomyces are well known to produce a large number of diverse antibiotic compounds. New environments are being explored and some new Streptomyces strains have been discovered; the potential of the plant endophytic environments has been highly explored during the last decade. In the present study, an endophytic actinomycete, VITGV01, was isolated from a farm tomato plant collected from an agricultural field. These actinomycetes are cultured in ISP2 medium supplemented with nystatin (15µg/ml) and nalidixic acid (50µg/ml). Based on the 16S rRNA analysis and physiological tests, this isolate was found to be a member of the known genus Streptomyces spp. and suggesting that it might be a new spp. The isolated strain showed morphological and chemical characteristics of the genus Streptomyces. The strain VITGV01 produced different antibiotics on different media such as yeast extract, malt extract, glucose medium, which were active against some Gram-positive and negative bacteria [Bacillus subtilis (MTCC2756), Staphylococcus aureus (MTCC737), Escherichia coli – (MTCC1687), and Klebsiella pneumoniae (MTCC109)]. The primary and secondary screening was performed against these human pathogens. Maximum zone of inhibition was observed against K. pneumoniae (22 mm) and minimal zone of inhibition against B. subtilis (8 mm). Maximum biomass production was recorded in media containing 1% sucrose and 1% yeast extract cultured at pH 7.0 for 7 days at 30◦C. FTIR analysis revealed amine, alkane (C = C) of the aromatic ring, secondary alcohol, and alkyl halide groups. The GC–MS data showed twenty compounds, of which fifteen are antimicrobial compounds.

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“…For the isolation of rhizosphere bacteria, one gram of soil was dissolved in 9 mL of PBS. The tube was left in incubation for 15 min at 70 °C, after which 100 µL were inoculated in AFM, ACA, AEL, ISP2, and ISP4 agar, supplemented with 50 µg/mL nalidixic acid and 50 µg/mL cyclohexamide and incubated at 28 °C for three weeks [ 20 , 21 ]. Isolated colonies were preserved in GYM Streptomyces broth, supplemented with 30% glycerol at −70 °C, until use.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma Cells

Romero-Arguelles

Romo-Sáenz

Morán-Santibañez

et al. 2022

IJERPH

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Plant-associated microorganisms represent a potential source of new antitumor compounds. The aim of the present study was to isolate endophytic and rhizosphere Gram-positive bacteria from Ibervillea sonorae and produce extracts with antitumor activity. Methanol and ethyl acetate extracts were obtained from 28 d bacterial fermentation, after which murine L5178Y-R lymphoma cells growth inhibition was evaluated at concentrations ranging from 15.62 µg/mL to 500 µg/mL by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide reduction colorimetric assay. IC50 and the selectivity index (SI) were calculated and compared with healthy control human peripheral blood mononuclear cells (PBMC). Identification of the isolated strains was performed using the 16S ribosomal gene and by MALDI-TOF MS mass spectrometry. The endophytic and rhizosphere bacterial extracts from strains ISE-B22, ISE-B26, ISE-B27, ISS-A01, ISS-A06, and ISS-A16 showed significant (p < 0.05) L5178Y-R cell growth inhibition, compared with an untreated control. The rhizosphere Micromonospora echinospora isolate ISS-A16 showed the highest (90.48%) percentage of lymphoma cells growth inhibition and SI (19.1) for PBMC, whereas the Bacillus subtilis ISE-B26 isolate caused significant (p < 0.01) growth inhibition (84.32%) and a SI of 5.2. Taken together, results of the present study evidenced antitumor effects by I. sonorae endophytic and rhizosphere bacteria culture extracts. Further research will involve the elucidation of the compounds that exert the antitumor activity and their evaluation in pre-clinical studies.

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Isolation and phenotypic characterization of actinomycetes from Rabat neighborhood soil and their potential to produce bioactive compounds

Cited by 8 publications

References 21 publications

Antimicrobial Potential of an Actinomycete Gordonia terrae JSN1.9-Derived Orange Pigment Extract

Antimicrobial Potential of an Actinomycete Gordonia terrae JSN1.9-Derived Orange Pigment Extract

Extraction and Characterization of Antibiotic Compounds produced by Streptomyces spp. VITGV01 against Selected Human Pathogens

In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma Cells

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