Isolation and Identification of Ruminal Methanogens from Grazing Cattle

Jarvis, Graeme N.; Strömpl, Carsten; Burgess, D M; Skillman, Lucy; Moore, Edward R. B.; Joblin, K. N.

doi:10.1007/s002849910065

Cited by 115 publications

(72 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to some literature data, predominant methanogens in the rumen ecosystem of farm animals include microorganisms of the genus Methanobrevibacter and Methanosaricina, and especially the following species: Methanobacterium formicicum, Methanobrevibacter ruminantium, Methanosaricina barkeri, Methanosaricina mazei -E-mail: adamck@jay.up.poznan.pl and Methanomicrobium mobile (Stewart et al, 1997;St-Pierre and Wright, 2012). Other data demonstrated predominance of only M. ruminantium (Leahy et al, 2010), whereas other data indicated the order Methanobacteriales as predominant in the rumen (Jarvis et al, 2000). However, the literature is still expanding and newer methanogens are being identified (Wright et al, 2008;Zhou et al, 2009;King et al, 2011;Lee et al, 2013).…”

Section: Characteristics Of the Rumen Ecosystem In Relation To Methanmentioning

confidence: 99%

Plant components with specific activities against rumen methanogens

Cieślak

Szumacher-Strabel

Stochmal

et al. 2013

Animal

150

102

View full text Add to dashboard Cite

A wide range of plant bioactive components (phytochemicals) have been identified as having potential to modulate the processes of fermentation in the rumen. The use of plants or plant extracts as natural feed additives has become a subject of interest not only among nutritionists but also other scientists. Although a large number of phytochemicals (e.g. saponins, tannins and essential oils) have recently been investigated for their methane reduction potential, there have not yet been major breakthroughs that could be applied in practice. A key tenet of this paper is the need for studies on the influence of plant components on methane production to be performed with standardized samples. Where there are consistent effects, the literature suggests that saponins mitigate methanogenesis mainly by reducing the number of protozoa, condensed tannins both by reducing the number of protozoa and by a direct toxic effect on methanogens, whereas essential oils act mostly by a direct toxic effect on methanogens. However, because the rumen is a complex ecosystem, analysis of the influence of plant components on the populations of methanogens should take into account not only the total population of methanogens but also individual orders or species. Although a number of plants and plant extracts have shown potential in studies in vitro, these effects must be confirmed in vivo.Keywords: ruminants, methane, tannins, saponins, essential oils ImplicationsThis review demonstrates that plant phytochemicals can have important effects on rumen methanogens, either by affecting methanogens directly and/or indirectly by affecting rumen protozoa. Modulation of the rumen microbiota, including methanogens and protozoa, with plant extracts such as saponins, tannins and essential oils has implications in improvement of animal nutrition through decreased dietary energy loss, and in limitation of the negative impact on the environment through mitigation of methane production.

show abstract

Section: Characteristics Of the Rumen Ecosystem In Relation To Methanmentioning

confidence: 99%

Plant components with specific activities against rumen methanogens

Cieślak

Szumacher-Strabel

Stochmal

et al. 2013

Animal

150

102

View full text Add to dashboard Cite

show abstract

“…Proper functioning of the digestive tract of ruminants is closely linked to the abundance and composition of the microbiota in the forestomach, which is a complex ecosystem comprising a vast diversity of bacteria, protozoa and fungi (6). Microorganisms making up the microflora of the rumen, and in particular the interactions between them and physiological processes in the body, directly affect the animal's physical condition and state of health (3,9,17,28). However, our knowledge of the rich diversity of microbial species in the rumen and our 1) Study financed by the General Directorate of the State Forests National Forest Holding in Poland (ZKE-U-6).…”

Section: Praca Oryginalnamentioning

confidence: 99%

“…The available literature contains numerous reports on the microbiota of domesticated ruminants and its effect on their well-being (7,17,24,35), but there is very little information on free-living ruminants, particularly with respect to the effect of the type of food consumed on parameters of the forestomach microbiota. Red deer is a species commonly found in Poland (4,13).…”

Section: Effect Of Balanced Supplementary Feeding In Wintermentioning

confidence: 99%

Effect of balanced supplementary feeding in winter on qualitative and quantitative changes in the population of microbes colonizing the rumen of red deer

Gnat

Dziedzic

Nowakiewicz

et al. 2018

Medycyna Weterynaryjna

View full text Add to dashboard Cite

Gnat S., Dziedzic R., Nowakiewicz A., Zięba P., Trościańczyk A., Majer-Dziedzic B., Ziółkowska G., Beeger S., Wójcik M.Effect of balanced supplementary feeding in winter on qualitative and quantitative changes in the population of microbes colonizing the rumen of red deer Summary Ruminants are a group of animals that process and assimilate their food in a unique manner. The functioning of the digestive tract of these animals is closely related to the abundance and composition of microbes in the forestomach, which is a complex ecosystem of bacteria, protozoa and fungi. Microorganisms present in the rumen, and in particular their effect on physiological processes in the body, influence the animal's physical condition and state of health. Microbiological examination of rumen microbiota ecology is hindered by a lack of selective growth media, as well as by difficulties in isolating bacteria in vitro and accurately identifying them. The aim of the study was to evaluate the effect of food consumed by red deer (Cervus elaphus) on the diversity of their rumen microbiota. Microbes were compared in two study periods. In autumn the animals' diet came exclusively from natural plant sources, while in winter, supplementary feeding was introduced, including specially prepared fodder. The study showed that in deer that did not receive the special fodder in winter, but only natural plant components, the abundance of bacterial flora decreased significantly compared with what it was in autumn, unlike in animals that did receive the fodder, whose composition and caloric value substantially increased the activity of rumen microbes. In winter, changes in proportions of different morphological forms of rumen bacteria were observed, as well as a decline in their total number, particularly in the animals that did not receive the pellets. A similar decline was also observed in the populations of yeasts and protozoa in winter. To sum up the results of the study, the use of the specially prepared high-calorie fodder in winter was shown to influence the rumen ecosystem of red deer. The most significant factor improving the condition of deer receiving supplementary fodder during this period is the stabilization of bacterial flora in the rumen, which directly contributes to the efficiency of digestion.

show abstract

“…Archaeal methanogens are obligate anaerobes (38), and species of the order Methanobacteriales are the most common methanogens found in the rumen (11). Recent studies using culture-independent methods investigating the methanogenic communities in the rumens of sheep and cattle have identified 21 different strains belonging to 13 species in sheep (40,41,43,44) and 13 different strains related to 8 species in cattle (23,37,42).…”

mentioning

confidence: 99%

Assessment of the Microbial Ecology of Ruminal Methanogens in Cattle with Different Feed Efficiencies

Zhou¹,

Hernandez-Sanabria²,

Guan³

2009

Appl Environ Microbiol

294

226

View full text Add to dashboard Cite

Cattle with high feed efficiencies (designated "efficient") produce less methane gas than those with low feed efficiencies (designated "inefficient"); however, the role of the methane producers in such difference is unknown. This study investigated whether the structures and populations of methanogens in the rumen were associated with differences in cattle feed efficiencies by using culture-independent methods. Two 16S rRNA libraries were constructed using ϳ800-bp amplicons generated from pooled total DNA isolated from efficient (n ‫؍‬ 29) and inefficient (n ‫؍‬ 29) animals. Sequence analysis of up to 490 randomly selected clones from each library showed that the methanogenic composition was variable: less species variation (22 operational taxonomic units [OTUs]) was detected in the rumens of efficient animals, compared to 27 OTUs in inefficient animals. The methanogenic communities in inefficient animals were more diverse than those in efficient ones, as revealed by the diversity indices of 0.84 and 0.42, respectively. Differences at the strain and genotype levels were also observed and found to be associated with feed efficiency in the host. No difference was detected in the total population of methanogens, but the prevalences of Methanosphaera stadtmanae and Methanobrevibacter sp. strain AbM4 were 1.92 (P < 0.05) and 2.26 (P < 0.05) times higher in inefficient animals, while Methanobrevibacter sp. strain AbM4 was reported for the first time to occur in the bovine rumen. Our data indicate that the methanogenic ecology at the species, strain, and/or genotype level in the rumen may play important roles in contributing to the difference in methane gas production between cattle with different feed efficiencies.Microbial fermentation and ruminal nutrient absorption are key steps in the energy metabolism of cattle. The microbiota in the rumen is highly associated with the diet, age, antibiotic use, and health of host animals (32). Different types of symbiotic anaerobic microorganisms, including bacteria, archaea, ciliated protozoa, and fungi, inhabit the rumen (15), interact with each other, and play important roles in affecting the host's performance. The microbial-host relationships are highly complex and varied, ranging from mutually beneficial cooperation to competition (10). Among ruminal microbes, bacteria decompose the feed into short-chain (C 1 to C 5 ) fatty acids, amino acids, H 2 , and CO 2 , etc. (20). To maintain the low hydrogen level in this habitat, hydrogen-utilizing microbes, such as methanogens, utilize H 2 and carbon substrates, mainly CO 2 , acetate, or methanol, to generate methane gas and hence to reduce hydrogen pressure in the rumen (8). However, this process causes a significant (6%) loss of dietary energy in the form of methane emission (14), which contributes to 13 to 19% of global greenhouse gas (16), and is one of the significant agricultural "causative sectors" contributing to global warming (13). Therefore, the energy loss and the consequent methane emission arouse both nutritional an...

show abstract

Isolation and Identification of Ruminal Methanogens from Grazing Cattle

Cited by 115 publications

References 28 publications

Plant components with specific activities against rumen methanogens

Plant components with specific activities against rumen methanogens

Effect of balanced supplementary feeding in winter on qualitative and quantitative changes in the population of microbes colonizing the rumen of red deer

Assessment of the Microbial Ecology of Ruminal Methanogens in Cattle with Different Feed Efficiencies

Contact Info

Product

Resources

About