Isolation and Identification of Potential Phosphate Solubilizing Bacteria from the Rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh

Islam, Tofazzal; Deora, Abhinandan; Hashidoko, Yasuyuki; Rahman, M. A.; Ito, Toshiaki; Tahara, Satoshi

doi:10.1515/znc-2007-1-218

Cited by 67 publications

(45 citation statements)

References 28 publications

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“…The ratios for bacteria in this study were much higher than 1.17-2.96 for phosphate-solubilising fungi in asparagus root zone [17]. Islam et al [18] reported the phosphate solubilization index of the rice isolates varied from 1.2 to 6.7. Many species of rhizobacteria including Bacilli, Pseudomonas and Klebsiella can solubilise insoluble phosphates in the agar assay in vitro [19][20].…”

Section: Phosphate Solubilization Activitycontrasting

confidence: 50%

Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004

Bhardwaj¹,

Shah²,

Joshi³

et al. 2017

J App Biol Biotech

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Klebsiella species known to exhibit important PGP traits like solubilization of phosphate, phytohormone production and good germination potential. In present study, based on the Phosphate solubilization and IAA production bacterial strain VRE36 was selected among the isolates collected from different sugarcane cultivar rhizosphere growing near Bardoli area for characterization and molecular identification through 16S rRNA gene sequence, which confirms the isolate as Klebsiella pneumoniae. The phosphate solubilization index of the isolate recorded was 3.9 and quantitative estimation reveals 17.4±1.78µg/ml release of phosphate in NBRIP broth. The high amount of IAA produced was 45.32±2.46µg/ml after 96 hour incubation at 37 °C. In seed germination assay with V radiate, C tetragonoloba and V unguiculata, treatments with bacteria were supported good plant height, dry weight and fresh weight when compared with controls. Maximum percentage of germination was recorded in V radiate (97.78%). The improved seedling parameters of the inoculated crop seeds indicated the potential of this isolate to be used in a bio-fertilizer formulation for sustainable production.

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Section: Phosphate Solubilization Activitycontrasting

confidence: 50%

Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004

Bhardwaj¹,

Shah²,

Joshi³

et al. 2017

J App Biol Biotech

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“…The suspended samples were then diluted from 10-1 to 10-8 [8], plated to Pikovskaya's agar medium (PAM) and incubated at 30°C for isolation and the screening process. The presence of PSB in the PAM medium is indicated by a clear zone around colonies, grown after 48 hours of incubation.…”

Section: Methodsmentioning

confidence: 99%

Phosphate Solubilizing Bacteria Adaptive to Vinasse

2015

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Abstract. Microorganisms identified as phosphate solubilizing bacteria (PSB) adaptive to vinasse were successfully screened from sugarcane soil from an agriculatural estate in Jatiroto. By conducting a screening on Pikovskaya's agar medium (PAM), we found that five different isolates were detected as PSB (pvk5a, pvk-5b, pvk-6b, pvk-7a, and pvk-8a). Of the five isolates only three could be grown and were found to be adaptive to vinasse based medium without any nutrients added (pvk-5a, pvk-5b and pvk-7a). The three isolates were characterized as coccus and Gram negative with no endospores detected. We suggest that these three isolates can be used as biofertilizer agent to support organic farming.

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“…Many of these efforts successfully resulted in the isolation of several microbial species capable of dissolving sparingly soluble P forms such as Acinetobacter sp., Klebsiella sp., Enterobacter sp., Microbacterium sp., and Pseudomonas sp. (Islam et al 2007). Ruangsanka (2014) recently reported the isolation of Buttiauxella izardii, Enterobacter cancerogenus, Burkholderia ubonensis, E. hormaechei, and Burkholderia pyrrocinia as PDB.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Pantoea Sp. Strain MK1D, and Erwinia Sp. Strain MK2Y as Tricalcium Phosphate Dissolving Bacteria Isolated from Calcareous Soil

K¹,

Il²

2017

Alexandria Science Exchange Journal: An International Quarterly

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This study aimed to isolate and characterize bacterial strains capable of solubilizing tricalcium phosphate (TCP) as the most commonly and insoluble phosphate in soils. Normal and molecular approaches were used to isolate and characterize phosphate dissolving bacteria (PDB) adapted to arid and semi-arid climate conditions. In this study, two strains were successfully isolated and taxonomically classified as Pantoea sp. strain MK1D, and Erwinia sp. strain MK2Y based on sequences of their 16S rRNA gene and were given GenBank accession numbers KU358676 and KU358677, respectively. This was confirmed by the formation of large halo zones on both Pikovskaya (PVK) and National Botanical Research Institute's Phosphate (NBRIP) culture media. The significant bacterial growth on liquid media was associated with the reduction of pH from 7.0 to 2.0 which lead to 83% solubilization of added TCP of 0.75 g/L. This study confirmed that both PVK and NBRIP media were reliable and comparable for the isolation of PDB and measuring their efficiency. Further studies are needed to confirm the effectiveness of these strains under pot and field conditions and their potential for commercial biofertilizers production.

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Isolation and Identification of Potential Phosphate Solubilizing Bacteria from the Rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh

Cited by 67 publications

References 28 publications

Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004

Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004

Phosphate Solubilizing Bacteria Adaptive to Vinasse

Characterization of Pantoea Sp. Strain MK1D, and Erwinia Sp. Strain MK2Y as Tricalcium Phosphate Dissolving Bacteria Isolated from Calcareous Soil

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