Isolation and Identification of Potential Bacillus Probiotics from Free Ranging Yaks of Tibetan Plateau, China

Li, Aoyun

doi:10.29261/pakvetj/2019.032

Cited by 12 publications

(9 citation statements)

References 20 publications

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“…T 1 in the feed of slow broiler chicks resulted in significant (P<0.05) improvement in growth performance parameters including body weight (Table 2) and feed conversion ratio (Table 3). These findings are in agreement with the previous studies (Walkunde et al, 2011;Bai et al, 2013;Zhang and Kim, 2014;Li et al, 2019). One of the most imperative attributes of probiotic bacteria is considered to be its effect on the immune response.…”

Section: Resultssupporting

confidence: 93%

Probiotic Attributes of Lactobacillus fermentum NKN51 Isolated from Yak Cottage Cheese and the Impact of Its Feeding on Growth, Immunity, Caecal Microbiology and Jejunal Histology in the Starter Phase of Broiler Birds

Yadav

Geeta

Pradhan

et al. 2020

IJAR

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Background: The discovery of antibiotics was a great success in control of the pathogenic infections in living beings. In addition, it also played a significant role in upliftment of the meat industry with their use as the growth promoters in the food animals including poultry. However, massive and incorrect use of antibiotics has resulted in the adverse outcome of antibiotic resistance over the years. In order to limit the antibiotic resistance at industry level itself, exploration of the possible alternatives such as probiotics is the need of the hour to replace the antibiotics in poultry industry without compromising the productivity. Hence, the present study was conducted to explore the effects of a novel probiotic bacterium, Lactobacillus fermentum NKN51 on the production performance of the broilers with an aim to replace the antibiotics in poultry production. Methods: In the present study, a total of 128 broiler birds were grouped randomly into four treatment groups which were fed basal diet (BD) along with the different levels of L. fermentum NKN51,viz.,T1 (BD+107cfu/gm), T2(BD+106cfu/gm), T3 (BD+105cfu/gm) and T4 or control (BD only) for a period of 28 days so as to observe the effect of Lactobacillus fermentum NKN51 in the starter phase of the broiler birds in terms of growth, immunity, gut health and physiology. Result: The result revealed that the group of broiler birds supplemented with Lactobacillus fermentum NKN51 at the level of 107cfu/gm of basal feed had significantly better growth performance, cell mediated and humoral immunity, gut health and jejunal histology in terms of villus height, VH:CD ratio and intestinal absorption capacity. Thus, Lactobacillus fermentum NKN51 was found as an ideal probiotic supplement exhibiting better potential in enhancing the efficiency of broiler birds in terms of improved productivity.

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Section: Resultssupporting

confidence: 93%

Probiotic Attributes of Lactobacillus fermentum NKN51 Isolated from Yak Cottage Cheese and the Impact of Its Feeding on Growth, Immunity, Caecal Microbiology and Jejunal Histology in the Starter Phase of Broiler Birds

Yadav

Geeta

Pradhan

et al. 2020

IJAR

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“…Before performing the animal experiment, some in vitro tests were performed to evaluate the antibacterial activity and safety of BS1, BS2 and BV1, including acid and bile salts tolerance tests, hemolytic activity tests, antibacterial tests, antibiotic susceptibility assay, and resistance gene tests. The results showed that BS1, BS2, and BV1 had the potential as probiotics [18]. The results of animal experiment demonstrate that the Bacillus subtilis isolated from Tibetan yaks can increase the growth performance of mice by improving digestive enzyme activity and the length of intestinal villi.…”

Section: Discussionmentioning

confidence: 99%

“…Two Bacillus subtilis strains (BS1 and BS2) and one Bacillus velezensis strain (BV1) were isolated from the intestines of yaks and were conserved in Laboratory of Veterinary Internal Medicine in Huazhong Agricultural University, Wuhan, China. Our previous study has demonstrated that BS1, BS2, and BV1 had high antibacterial efficacy against Staphylococcus aureus (ATCC 26112), Escherichia coli (ATCC 25922), and Salmonella enteritidis (NCTC 13349) and exhibited a high tolerance to acid and bile salts [18]. In addition, antibiotic resistance experiments, resistance gene tests, and hemolytic experiments were performed to determine the safety of the BS1, BS2, and BV1 strains.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, antibiotic resistance experiments, resistance gene tests, and hemolytic experiments were performed to determine the safety of the BS1, BS2, and BV1 strains. Our previous results showed that BS1, BS2, and BV1 had lower antibiotic resistance and resistance genes and hemolysis were not found [18]. The safety assessment results showed that the isolated strains were safe and could be used in animal experiments [18].…”

Section: Methodsmentioning

confidence: 99%

“…Our previous results showed that BS1, BS2, and BV1 had lower antibiotic resistance and resistance genes and hemolysis were not found [18]. The safety assessment results showed that the isolated strains were safe and could be used in animal experiments [18]. Three isolated strains were cultured in Luria–Bertani (LB) broth at 37 °C for 24 h under aerobic environment.…”

Section: Methodsmentioning

confidence: 99%

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Probiotics isolated from yaks improves the growth performance, antioxidant activity, and cytokines related to immunity and inflammation in mice

Wang

et al. 2019

Microb Cell Fact

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Background Yaks living in the high-altitude hypoxic environment of Tibetan plateau (3600 m) have special gut microbes. However, it is still little research on yak probiotics until now. Therefore, the purpose of our study was to evaluate the growth promoting effect, antioxidant capability, immune effect, and anti-inflammatory ability of Bacillus subtilis and Bacillus velezensis isolated from Tibetan yaks in mice model. Results The results showed that the isolated strains supplementation not only improve the growth performance but also increased the length of villus in the small intestine and intestinal digestive enzyme activity. Importantly, we observed that the T-AOC, SOD, and GSH-PX levels were increased and the MDA content was reduced in probiotic-treated mice, which implied that probiotics supplementation can ameliorate the antioxidative activity of mice. The levels of AST and ALT correlated with the hepatic injury were reduced and the levels of AKP, TP, GLB, ALB, Ca, and P were markedly higher than those in the control group. Additionally, mice treated with probiotics exhibited higher serum IgG, IgM and IgA, which can reflect the immune status to some extent. At the same time, the major pro-inflammatory factor TNF-α, IL-6, and IL-8 were down-regulated and the anti-inflammatory factor IL-10 was up-regulated compared with the control groups. Conclusions In conclusion, these results demonstrated that Bacillus subtilis and Bacillus velezensis supplementation can increase overall growth performance and ameliorate the blood parameters related to inflammation and immunity of mice. Electronic supplementary material The online version of this article (10.1186/s12934-019-1161-6) contains supplementary material, which is available to authorized users.

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Probiotic Potential of Bacillus licheniformis and Bacillus pumilus Isolated from Tibetan Yaks, China

Zeng

Zhang

et al. 2022

Probiotics & Antimicro. Prot.

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Isolation and Identification of Potential Bacillus Probiotics from Free Ranging Yaks of Tibetan Plateau, China

Cited by 12 publications

References 20 publications

Probiotic Attributes of Lactobacillus fermentum NKN51 Isolated from Yak Cottage Cheese and the Impact of Its Feeding on Growth, Immunity, Caecal Microbiology and Jejunal Histology in the Starter Phase of Broiler Birds

Probiotic Attributes of Lactobacillus fermentum NKN51 Isolated from Yak Cottage Cheese and the Impact of Its Feeding on Growth, Immunity, Caecal Microbiology and Jejunal Histology in the Starter Phase of Broiler Birds

Probiotics isolated from yaks improves the growth performance, antioxidant activity, and cytokines related to immunity and inflammation in mice

Probiotic Potential of Bacillus licheniformis and Bacillus pumilus Isolated from Tibetan Yaks, China

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