Isolation and identification of antialgal substances produced byPseudomonas aeruginosa

Dakhama, A.; Noüe, J. de la; Lavoie, Michel

doi:10.1007/bf02186232

Cited by 74 publications

(42 citation statements)

References 28 publications

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“…Thus, there appears to be a similarity between our results and those of Sharabi and Gilboa-Garber. On the other hand, our results and those of Dakhama et al (13) are not comparable. The active materials which we investigated had molecular masses of at least 5 kDa since they were found in the retentate after ultrafiltration experiments.…”

Section: Resultscontrasting

confidence: 99%

Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

Wigglesworth‐Cooksey

Cooksey

2005

Appl Environ Microbiol

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Biofilms dominated by pennate diatoms are important in fields as diverse as ship biofouling and marine littoral sediment stabilization. The architecture of a biofilm depends on the fact that much of its mass consists of extracellular polymers. Although most illuminated biofilms in nature are dominated by phototrophs, they also contain heterotrophic bacteria. Given the close spatial association of the two types of organisms, cell-cell interaction is likely. Fluorophore-conjugated lectins were used to demonstrate the sites of the various extracellular polymers in three species of diatoms. Based on their lectin staining properties, the polymers in different species appeared to be similar, but their involvement in the process of attachment to a surface differed. In a coculture Pseudoalteromonas sp. strain 4 or its sterilized spent medium reduced the ability of Amphora coffeaeformis and Navicula sp. strains 1 and D to adhere, inhibited motility, and caused agglutination and eventually diatom cell lysis. Diatoms could be protected from the negative effects of the bacterial spent medium if D-galactose or mannan was included in the incubation medium. The active principle of the spent medium is probably a lectin/agglutinin that is able to bind to the extracellular polymers of the diatoms that are involved in adhesion and motility. Awareness of interactions of this type is important in the study of natural biofilms.Marine biofilms are studied for both ecological and technological reasons. For instance, in the near-shore environment it is now well accepted that microorganisms, notably raphid diatoms, through their production of extracellular polymers, are at least partially responsible for the stabilization of the sediment (12). These polymers are secreted through pores in the silicon dioxide cell wall of the diatom and through the raphe structure, which is a longitudinal slit in the cell wall. Single raphes are found on the ventral and dorsal cell walls of some diatoms (e.g., Navicula sp.), whereas in other species (e.g., Amphora sp.) two raphes are on the ventral surface of the cell. Raphes have been shown to be a major connection between the cell membrane and the environment (39). It is conceivable that the loss of the diatom component of the sediment microflora could be responsible for the sediment erosion seen in salt marshes over the last 40 years (26) and may contribute to hypoxia in the near-shore environment (Cooksey and Wigglesworth-Cooksey, Abstr. 6th USEPA Int. Symp. Fish Physiol., Toxicol., Water Quality: Effects of Hypoxia, abstr. 15, 2001). There is currently no universally accepted view concerning which of the several exopolymers (37, 41) (Table 1) made by diatoms are responsible for the stabilization activity. However, in the case of Amphora coffeaeformis in laboratory simulations, only the matrix polymer appears to be involved in this activity (41).There are many reasons for this lack of consensus. In the first place, it is very difficult to compare work on extracellular polymers (extracellular polymeric ...

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Section: Resultscontrasting

confidence: 99%

Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

Wigglesworth‐Cooksey

Cooksey

2005

Appl Environ Microbiol

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“…Previous reports have demonstrated that many bacteria have a significant algicidal effect on several harmful algal bloom species (10,11,14,17). Most algicidal bacteria isolated from the environment are characterized as belonging to the genera Cytophaga, Saprospira, Pseudoalteromonas, and Alteromonas (55 Four types of these metabolites, surfactin and polyketides (difficidin, macrolactin, and bacillaene), are known for their antibacterial activity (24,30).…”

Section: Discussionmentioning

confidence: 99%

“…Bacteria that act indirectly exert killing activity through the production of extracellular algicidal substances, such as the phenazine pigments and 1-methyl-␤-carboline secreted by certain Pseudomonas spp. (14,15), ␤-cyano-L-alanine from Vibrio spp. (16), and lactones produced by Ruegeria pomeroyi (17).…”

mentioning

confidence: 99%

Bacilysin from Bacillus amyloliquefaciens FZB42 Has Specific Bactericidal Activity against Harmful Algal Bloom Species

Chen

et al. 2014

Appl Environ Microbiol

102

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bHarmful algal blooms, caused by massive and exceptional overgrowth of microalgae and cyanobacteria, are a serious environmental problem worldwide. In the present study, we looked for Bacillus strains with sufficiently strong anticyanobacterial activity to be used as biocontrol agents. Among 24 strains, Bacillus amyloliquefaciens FZB42 showed the strongest bactericidal activity against Microcystis aeruginosa, with a kill rate of 98.78%. The synthesis of the anticyanobacterial substance did not depend on Sfp, an enzyme that catalyzes a necessary processing step in the nonribosomal synthesis of lipopeptides and polyketides, but was associated with the aro gene cluster that is involved in the synthesis of the sfp-independent antibiotic bacilysin. Disruption of bacB, the gene in the cluster responsible for synthesizing bacilysin, or supplementation with the antagonist N-acetylglucosamine abolished the inhibitory effect, but this was restored when bacilysin synthesis was complemented. Bacilysin caused apparent changes in the algal cell wall and cell organelle membranes, and this resulted in cell lysis. Meanwhile, there was downregulated expression of glmS, psbA1, mcyB, and ftsZ-genes involved in peptidoglycan synthesis, photosynthesis, microcystin synthesis, and cell division, respectively. In addition, bacilysin suppressed the growth of other harmful algal species. In summary, bacilysin produced by B. amyloliquefaciens FZB42 has anticyanobacterial activity and thus could be developed as a biocontrol agent to mitigate the effects of harmful algal blooms. Eutrophication of surface waters has many undesirable effects and can lead to major water quality issues in freshwater and coastal systems (1). This phenomenon results in blooms of harmful algal species in freshwater lakes and brackish waters throughout the world. Moreover, the excessive growth of harmful algae, such as microalgae and cyanobacteria, often increases the production of inherent toxins such as microcystins and nodularins that cause acute poisonings of fish, birds, and mammals, including humans (2). For example, dogs died after they were exposed to a cyanobacterial bloom of Microcystis aeruginosa in Lake Amstelmeer (The Netherlands), and the concentration of microcystin in this lake was up to 5.27 ϫ 10 3 g g Ϫ1 of dry weight (3). In recent years, harmful algal blooms of eutrophic water in China have occurred frequently, including in Lake Taihu and Lake Chaohu, and Microcystis is thought to be the dominant bloom genera (4).Many control techniques have been used to prevent and mitigate bloom problems, including yellow loess (5), clay (6), and chemical agents such as copper sulfate and hydrogen peroxide (7). However, each of the physical and chemical methods available to remediate eutrophic water is associated with certain disadvantages (8). Therefore, there is still a pressing need for environmentally friendly, cost-effective, and convenient bactericidal agents directed against cyanobacterial blooms in eutrophic lakes. Biological control agents such as bact...

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“…Other remaining compounds were inactive. In study of antibacterial activities for phenazine alkaloids, all compounds of the phenazine class have been reported to have antibacterial properties (Dahiya et al, 1988;Dakhama et al, 1993;Jayatilake et al, 1996). These results suggest the phenazine carboxylic acid 1 could possess antimicrobial activity.…”

Section: Resultsmentioning

confidence: 99%

Isolation of secondary metabolites from an Arctic bacterium, Pseudomonas aeruginosa and their antimicrobial activities

Youn¹,

Kim²,

Han³

et al. 2016

The Korean Journal of Microbiology

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Chemical study of an Arctic bacterium, Pseudomonas aeruginosa (Pseudomonadaceae) led to the isolation of two diketopiperazines 1 and 2, two phenazine alkaloids 3 and 4, and an indole carbaldehyde 5, along with a benzoic acid derivative 6. The structures of the compounds were confirmed by 1D and 2D NMR, and MS experiments, as well as by comparison of their data with published values. Among the isolates, compounds 5 and 6 were isolated for the first time from P. aeruginosa of the seawater of Arctic Chuckchi Sea. Antimicrobial activities of compounds 1-6 against a Staphylococcus aureus and Candida albicans were evaluated.

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Isolation and identification of antialgal substances produced byPseudomonas aeruginosa

Cited by 74 publications

References 28 publications

Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

Bacilysin from Bacillus amyloliquefaciens FZB42 Has Specific Bactericidal Activity against Harmful Algal Bloom Species

Isolation of secondary metabolites from an Arctic bacterium, Pseudomonas aeruginosa and their antimicrobial activities

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