Isolation and genotyping of Toxoplasma gondii from pregnant dairy cows (Bos taurus) slaughtered

Macedo, Madlaine Frigo Silveira Barbosa de; Macedo, César Augusto Barbosa de; Ewald, Maria Paula de Carvalho; Martins, Guilherme Felippelli; Zulpo, Dauton Luiz; Cunha, Ivo Alexandre Leme da; Taroda, Alessandra; Cardim, Sérgio Tosi; Su, Chunlei; Garcia, João Luís

doi:10.1590/s1984-29612012000100016

Cited by 18 publications

(15 citation statements)

References 23 publications

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“…Despite the presence of T. gondii has been confirmed in beef cattle in Brazil by serological studies (GarCia et al, 1999), PCR and sequencing (sanTos et al, 2010), and isolation by bioassay (maCeDo et al, 2012), the present investigation performed in aborted fetuses with lesions indicative of protozoal infection were negative for T. gondii DNA, in accordance to studies performed in many countries, where the rate of infected bovine fetus with T. gondii was low (Dubey, 1986).…”

Section: Palavras-chavesupporting

confidence: 88%

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

et al. 2013

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The aim of this study was to determine if Toxoplasma gondii was present in aborted bovine fetuses in Brazil. Histopathology of 105 cases with suspected infectious abortion, analyzed during the period from 2006 to 2008 at Centro de Pesquisa e Desenvolvimento de Sanidade Animal of Instituto Biológico, São Paulo, showed 75 cases with indications of abortion due to apicomplexan protozoa. These cases were submitted to PCR for verification of the laboratory diagnosis. Fetal DNA was extracted from central nervous system, heart, liver, muscle, and/or placenta samples to obtain a 529 bp DNA fragment. T. gondii DNA was not detected in any of the bovine fetuses analyzed, suggesting that it may not be a frequent cause of bovine abortion.

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Section: Palavras-chavesupporting

confidence: 88%

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

et al. 2013

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“…Genotype #1 is the clonal type II and is rare in Brazil but common in North America and Europe. The presence of type II has been reported in guinea fowl and cattle in Brazil (DUBEY et al, 2011;MACEDO et al, 2012). Previous studies on free-living pigeons (C. livia) in Brazil did not obtain T. gondii isolates (GODOI et al, 2010;LIMA et al, 2011).…”

Section: Discussionmentioning

confidence: 94%

Genetic characterization of Toxoplasma gondii isolates from eared doves (Zenaida auriculata) in Brazil

Barros

Taroda

Zulpo

et al. 2014

Rev. Bras. Parasitol. Vet.

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Eared doves (Zenaida auriculata), which are common in urban, rural and wild areas in many regions of Brazil, are frequently prey for domestic cats. Therefore Toxoplasma gondii isolates obtained from doves may reflect greater environmental diversity than those from other hosts. The aim of the present study was to evaluate T. gondii seroprevalence, isolate and genotype strains from Z. auriculata. Serum and tissue samples were collected from 206 doves for use in the modified agglutination test (MAT) and mouse bioassay. The prevalence of T. gondii antibodies in the doves was 22.3% (46/206), with titers ranging from 16 to 4096, and T. gondii strains were isolated from 12 of these doves. Five genotypes were detected by means of PCR-RFLP, including ToxoDB genotypes #1, #6, #17 and #65, and one genotype that had not previously been described (ToxoDB#182). This was the first report on isolation of T. gondii from Z. auriculata. This study confirmed the genetic diversity of T. gondii isolates and the existence of clonal type II (ToxoDB genotype #1) in Brazil.Keywords: Toxoplasma gondii, eared doves, genotyping, PCR-RFLP, MAT, Biossay. ResumoPombos silvestres (Zenaida auriculata), comuns em áreas urbanas, rurais e selvagens em muitas regiões do Brasil, são frequentemente predados por gatos domésticos. Sendo assim, os isolados de T. gondii obtidos de pombos podem refletir uma maior diversidade ambiental do que os outros hospedeiros. O objetivo do presente estudo foi avaliar a soroprevalência, isolar e genotipar T. gondii de Z. auriculata. Amostras de soro e tecido foram coletadas de 206 pombos para o teste de aglutinação modificado (MAT) e o bioensaio em camundongos. A prevalência de anticorpos contra T. gondii em pombos foi 22,3% (46/206), com títulos variando de 16 a 4096, e T. gondii foi isolado de 12 pombos. Cinco genótipos foram detectados por PCR-RFLP, incluindo os genótipos ToxoDB #1, #6, #17, #65 e um genótipo não descrito anteriormente (ToxoDB#182). Esse é o primeiro relato de isolamento de T. gondii de Z. auriculata. Este estudo também confirmou a diversidade dos isolados de T. gondii e a presença de tipo clonal II (ToxoDB #1) no Brasil.

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“…Those authors concluded that there is a high diversity in the strains found in Brazil, and none of them observed the type II genotype. However, Macedo et al (2012) and Da Silva et al (2011) recently described the type II genotype in cattle and sheep in Brazil, respectively.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses

Evers

Garcia

Navarro

et al. 2013

Rev. Bras. Parasitol. Vet.

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This study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. We collected brain and blood samples from 398 horses of various ages, from six Brazilian states. Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. Among the 398 horses, positivity for T. gondii was identified in 46 (11.6%) by IFAT and in 14 (3.5%) by mouse bioassay. In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rDNA to differentiate among T. gondii, Neospora caninum, and Sarcocystis neurona, we found that two of the 14 brains were positive for T. gondii only. For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. Our results show that some horses sent to slaughter in Brazil have been exposed to T. gondii.Keywords: Slaughterhouse, horses, Toxoplasma gondii, bioassay, indirect fluorescent antibody test, nested PCR-RFLP. ResumoO objetivo do estudo foi investigar anticorpos anti-Toxoplasma gondii e isolar o parasita do cérebro de equídeos abatidos em matadouros-frigoríficos no Brasil. Colheram-se amostras de 398 cérebros e sangue de equídeos machos e fêmeas de idades variadas, provenientes de seis estados brasileiros. As amostras de soro foram avaliadas pelo teste de imunofluorescência indireta (IFI) para T. gondii (ponto de corte ≥ 64), e os fragmentos de cérebros foram submetidos ao bioensaio em camundongos. Por meio da IFI, 46 (11,6%) equídeos foram soropositivos. Pelo bioensaio em camundongos, 14 (3,5%) cérebros de equídeos testados foram positivos. Em doze dos bioensaios, os camundongos foram positivos somente pela IFI (ponto de corte ≥ 16) e T. gondii foi isolado nos outros dois bioensaios. A PCR-RFLP com base em 18S rDNA para diferenciar entre T. gondii, Neospora caninum, e Sarcocystis neurona foram feitas em todos os 14 cérebros e dois foram positivos apenas para T. gondii. De dois isolados positivos para T. gondii e do cérebro positivo à PCR em que realizou-se a PCR-RFLP, com base em 13 marcadores e SAG2, a genotipagem mostrou ser o T. gondii tipo I para todas as amostras. A IFI de soros de equídeos e do bioensaio em camundongos identificaram positividade em 60 (15%) amostras testadas. Os resultados mostram que alguns cavalos enviados para abate foram expostos ao T. gondii.

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Isolation and genotyping of Toxoplasma gondii from pregnant dairy cows (Bos taurus) slaughtered

Cited by 18 publications

References 23 publications

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

Genetic characterization of Toxoplasma gondii isolates from eared doves (Zenaida auriculata) in Brazil

Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses

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