Isolation and genome analysis of a lyticPasteurella multocidaBacteriophage PMP-GAD-IND

Abstract

The present work is a part of research for the development of an improved phage lysate marker vaccine and a companion DIVA assay against haemorhagic septicaemia. This study describes the isolation and genome analysis of PMP-GAD-IND a lytic Pasteurella multocida bacteriophage.

Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections

“…Electron microscopy showed that the appearance of PHB01 was composed of an isometric polyhedral head approximately 55 nm in diameter and a short tail ~13 nm in length (Figure 2B). These morphological characteristics are quite similar to phage PHB02, a lytic P. multocida phage belonging to the family Podoviridae in the order Caudovirales [17], but differ from the reported Pasteurella phages in family Siphoviridae , which have long noncontractile tails (≥100 nm) [18,39,40] and/or in the family Siphoviridae with long, contractile tails (≥100 nm) [19]. These findings suggest that PHB01 is a member of the family Podoviridae in the order Caudovirales.…”

“…Since then, there have been no reports on P. multocida phages for 12 years. In 2018, the first complete genome sequences of lytic bacteriophages for P. multocida capsular types A and B were reported [17,18]. However, there is still a lack of reporting on lytic bacteriophages for P. multocida strains of other capsular types (D, E, and F).…”

“…It has been reported that more than 95% of all phages described in the literature belong to the order Caudovirales (tailed ds-DNA phages), which comprises three families: the families Myoviridae (viruses with contractile tails), Siphoviridae (viruses with long, noncontractile tails), and Podoviridae (viruses with short noncontractile tails) [18,38]. Viruses in different families have icosahedral or oblate heads, but differ in the length and contractile abilities of their tails [38].…”

“…P. multocida phages were first reported in 1956 [16], however, the lytic P. multocida phages had not been characterized until 2018 [17,18]. To date, there have been only five whole genome sequences of P. multocida phages available in the GenBank database including the three temperate transducing Pasteurella phages F108 (Accession: NC_008193) [19], AFS-2018a (Accession: MH238466), and Pm86 (Accession: MH238467) [20], and two lytic phages PMP-GADVASU-IND (Accession: KY203335) [18], and PHB02 (Accession: MF034659) [17]. While lytic phages specific for P. multocida capsular types A and B have been characterized recently [17,18], there is still a lack of information on lytic phages for other P. multocida capsular types.…”

“…To date, there have been only five whole genome sequences of P. multocida phages available in the GenBank database including the three temperate transducing Pasteurella phages F108 (Accession: NC_008193) [19], AFS-2018a (Accession: MH238466), and Pm86 (Accession: MH238467) [20], and two lytic phages PMP-GADVASU-IND (Accession: KY203335) [18], and PHB02 (Accession: MF034659) [17]. While lytic phages specific for P. multocida capsular types A and B have been characterized recently [17,18], there is still a lack of information on lytic phages for other P. multocida capsular types. In this study, a novel lytic T7-like phage designated vB_PmuP_PHB01 (hereafter referred to as phage PHB01) specific for P. multocida capsular type D strains was isolated and characterized.…”