Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method

Yesilmen, Simten; Vural, Aydın; Erkan, Mehmet Emin; Yildirim, Ibrahim Halil

doi:10.17221/69/2016-vetmed

Cited by 3 publications

(10 citation statements)

References 31 publications

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“…Our results reveal a larger occurrence of Arcobacter in chicken than in cattle (Table -2), in accordance with the previous findings [22,24,26,28]. Our results also proved greater contamination levels of Arcobacter spp.…”

Section: Discussionsupporting

confidence: 93%

“…High resistance rates to T, AMP, CX, and E previously have been observed in Arcobacter spp. isolated from domestic geese and animal feces in Turkey, with rates ranging from 61.4% to 100% [26,32], as well as high rates of resistance tot T, AMP, and E in A. butzleri from pets in Malaysia, up to 80% [41]. In contrast with the results of our study, susceptibility to T, E, and AMP has been detected previously in Turkey, Belgium, and Japan, ranging from 78.7% to 100% [32,42,43].…”

Section: Discussioncontrasting

confidence: 93%

“…This result corresponds with that of many previous findings [2,5,22,25,27]. However, other investigators have stated that A. cryaerophilus was more common than A. butzleri [24,26].The dissimilarities in the occurrence of Arcobacter spp. maybe attributed to the different processing plant conditions and procedures, seasonal variances, geographical locations, experimental designs and analyses, and water sources [30,31].…”

Section: Discussionsupporting

confidence: 91%

“…Finding Arcobacter spp. in dissimilar sources such as humans, bird carcasses, meat, and the environment has publicized the issue of drug resistance [26], and our results support this finding.…”

Section: Discussionsupporting

confidence: 86%

“…from 14.3% of the food samples retailed in Sicily, finding A. butzleri and A. cryaerophilus at rates of 92.3% and 7.7%, respectively. Other studies conducted in Belgium [24], Malaysia [25], and Turkey [26] have found the occurrence of Arcobacter spp. in retail minced meat, in various other sources, and in animal fecal samples 9%, 15%, and 13%, respectively.…”

Section: Discussionmentioning

confidence: 86%

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Prevalence, resistance to antimicrobials, and antibiotypes of Arcobacter species recovered from retail meat in Wasit marketplaces in Iraq

Kanaan

2021

Int J One Health

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Background and Aim: Arcobacter is a food-borne pathogen associated with human and animal infections. In Iraq, these infections and their adverse effects on public health have not been well investigated. For this reason, as well as to submit data on the resistance to antimicrobials and antibiotypes of the Arcobacter spp. and their occurrence in retail meat in the Wasit marketplaces, this study was undertaken. Materials and Methods: A total of 83 samples of fresh raw (n=35) and chilled meat (n=48) were purchased randomly from marketplaces in various regions of the Wasit Governorate. Bacterial detection was conducted using cultural methods, biochemical analysis, and the Oxoid Biomedical Identification System Campy. Confirmation of these bacteria at the species level was performed using the multiplex polymerase chain reaction method. Susceptibility of the Arcobacter spp. to antimicrobials was investigated in 11 isolates comprising Arcobacter butzleri (n=9) and Arcobacter cryaerophilus (n=2) using the Kirby–Bauer disk diffusion method. Results: A total of 32 (38.6%) of the 83 fresh raw and chilled meat samples tested positive for Arcobacter spp.; of those, 27 (84.4%) and 5 (15.6%) were recognized as A. butzleri and A. cryaerophilus, respectively. Maximum resistance was perceived, respectively, to tetracycline, erythromycin, and ampicillin (90.9%, 81.8%, and 81.8%, respectively). In contrast, a low resistance rate against fluoroquinolones up to 9.09% was found. Antibiograms of the A. butzleri and A. cryaerophilus isolates yielded ten antibiotypes. The vast majority of the isolates (90.91%) were resistant to no fewer than three classes of antimicrobials, and 27.3% of these showed resistance to six antibiotics. A total of 91% of the analyzed isolates had a multiple antibiotic resistance index score between 0.27 and 0.73. Conclusion: Our outcomes demonstrated that retail meat can be a prospective vehicle for pathogenic Arcobacter, making these products a possible risk to human health. Our outcomes postulate that the contamination of retail meats by pathogenic Arcobacter is a global public health concern, particularly with the growing resistance to life-saving drugs, and emphasizes consumer understanding about the quality and safety of these products. To achieve healthy food products, good management practices, and successful control approaches must be implemented across the entire food chain, not only to protect consumers from these contaminants but also to minimize the risk of drug resistance.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 86%

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Prevalence, resistance to antimicrobials, and antibiotypes of Arcobacter species recovered from retail meat in Wasit marketplaces in Iraq

Kanaan

2021

Int J One Health

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Resistance rate and novel virulence factor determinants of Arcobacter spp., from cattle fresh meat products from Iraq

Jasim

Al-Abodi

Ali

2021

Microbial Pathogenesis

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The Prevalence of Aliarcobacter Species in the Fecal Microbiota of Farm Animals and Potential Effective Agents for Their Treatment: A Review of the Past Decade

2022

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There is an endless demand for livestock-originated food, so it is necessary to elucidate the hazard points for livestock breeding. Pathogens are one of the hazard points that threaten the biosecurity of farm-animal breeding and public health. As a potential foodborne pathogen, Aliarcobacter is a member of the intestinal microbiota of farm animals with and without diarrhea. Aliarcobacter spp. are capable of colonizing livestock intestines and are transmitted through the feces. Hence, they endanger slaughterhouses and milk products with fecal contamination. They also have other, rarer, vertical and horizontal transmission routes, including the offspring that abort in farm animals. Gastrointestinal symptoms and abort cases demonstrate potential financial losses to the industry. Viewed from this perspective, the global circulation of farm-animal products is a significant route for zoonotic agents, including Aliarcobacter. In the last decade, worldwide prevalence of Aliarcobacter in fecal samples has ranged from 0.8% in Italy to 100% in Turkey. Furthermore, antibiotic resistance is recognized as a new type of environmental pollutant and has become a hot topic in animal breeding and the food industry. Increasing antibiotic resistance has become a significant problem impacting productivity. The increase in antimicrobial resistance rates in Aliarcobacter is caused by the misuse of antimicrobial drugs in livestock animals, leading to the acquiring of resistance genes from other bacteria, as well as mutations in current resistance genes. The most resistant strains are A. butzleri, A. cryaerophilus, and A. skirrowii. This review analyzes recent findings from the past decade on the prevalence of Aliarcobacter in the intestinal microbiota and the current effective antibiotics against Aliarcobacter. The paper also highlights that A. cryaerophilus and A. skirrowii are found frequently in diarrheal feces, indicating that Aliarcobacter should be studied further in livestock diarrheal diseases. Moreover, Aliarcobacter-infected farm animals can be treated with only a limited number of antibiotics, such as enrofloxacin, doxycycline, oxytetracycline, and gentamicin.

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Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method

Cited by 3 publications

References 31 publications

Prevalence, resistance to antimicrobials, and antibiotypes of Arcobacter species recovered from retail meat in Wasit marketplaces in Iraq

Prevalence, resistance to antimicrobials, and antibiotypes of Arcobacter species recovered from retail meat in Wasit marketplaces in Iraq

Resistance rate and novel virulence factor determinants of Arcobacter spp., from cattle fresh meat products from Iraq

The Prevalence of Aliarcobacter Species in the Fecal Microbiota of Farm Animals and Potential Effective Agents for Their Treatment: A Review of the Past Decade

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