Isolation and characterization of tri‐ and tetra‐repeat microsatellite loci in the white‐spotted charr Salvelinus leucomaenis (Salmonidae)

Abstract

Tri- and tetra-motif repeat microsatellite marker loci were developed for the white-spotted charr Salvelinus leucomaenis. The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite-primer sets, available for the estimation of polymorphisms, were identified. The number of alleles in a wild population ranged from two to four and the observed and expected heterozygosities were 0·180-0·600 and 0·188-0·599, respectively.

Genetic characteristics of white-spotted char <i>Salvelinus leucomaenis</i> inhabiting Shimoyosaku tributary in Ozegahara

“…A: number of alleles, Ar: Allele richness, He: expected heterozygosity, Ho: observedheterozygosity. *Observed and expected heterozygosity of five locus (Saleu2, Saleu5, Saleu 8,Saleu 22, and Saleu25) and two locus (Sle1 and Sle4) in the population of Tone river were drawnfromYamamoto and Sekino (2015) andYamaguchi et al (2010), …”