Isolation and Characterization of Thermophilic Bacteria from Gavmesh Goli Hot Spring in Sabalan Geothermal Field, Iran: <i>Thermomonas hydrothermalis</i> and <i>Bacillus altitudinis</i> Isolates as a Potential Source of Thermostable Protease

Abdollahi, Pantea; Ghane, Maryam; Babaeekhou, Laleh

doi:10.1080/01490451.2020.1812774

Cited by 18 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. altitudinis has been isolated from various environments, including thermal pools (Abdollahi et al, 2021). Likewise, this species has been associated with good capacities to tolerate heavy metals, as well as excellent capacities to bioremediate environments contaminated with zinc, nickel, and copper (Khan et al, 2021; Babar et al, 2021; Yue et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Flores

Valencia-Marin

Chávez-Avila

et al. 2022

Preprint

View full text Add to dashboard Cite

The geothermal zone of Araró, México, is located within the trans-Mexican volcanic belt, an area with numerous arsenic (As)-rich hot springs. In this study, the draft genome sequence of two endemic Bacillus strains (ZAP17 and ZAP62) from Araró microbial mat hot springs was determined, which were able to grow on arsenate (up to 64 mM) and arsenite (up to 32 mM). Phylogenetic analysis based on 16S rRNA and gyrB sequences, as well as genome sequence analysis based on average nucleotide identity (>96%) and digital DNA–DNA hybridization (>70%), indicated that these strains belong to the Bacillus paralicheniformis ZAP17 and Bacillus altitudinis ZAP62. Furthermore, through genome mining, it was identified two arsenic resistance operons, arsRBC, and arsRBCDA in both strains as potential determinants of arsenic (As) resistance. Predicted ArsA (arsenial pump-driving ATPase), ArsB (Arsenical efflux pump protein), ArsC (Arsenate reductase), ArsD (Arsenical efflux pump protein) and ArsR (Metalloregulator/ars operon repressor) proteins, clearly grouped with their respective clades corresponding to other characterized bacterial species, mainly Firmicutes. To further evaluate the functionality of the ars operons in ZAP17 and ZAP62 strains, our results showed that arsRBC and arsRBCDA genes were expressed in the presence of arsenite (III). Finally, the presence of ars operons in the genome of Bacillus species residing in As-rich environments, such as the Araró hot springs, might be a potential mechanism to survive under such harsh conditions, as well as to design sustainable bioremediation strategies.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Flores

Valencia-Marin

Chávez-Avila

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Considering the use of a lipid-rich waste as a substrate for the PHAs accumulation, it is necessary for the hydrolysis of triglycerides into free fatty acids before their subsequent transformation to PHAs [ 98 ]. The bacteria that were potentially able to accumulate PHAs with a lipolytic activity were Acidovorax [ 99 ], Acinetobacter [ 100 ], Azospirillum [ 101 ], Chryseobacterium [ 102 ], Comamonas [ 103 ], Corynebacterium [ 104 ], Devosia [ 105 ], Dokdonella [ 106 ], Dyella [ 107 ], Ferruginibacter [ 108 ], Flavobacterium [ 109 ], Gemmobacter [ 77 ], Gordonia [ 110 ], Leifsonia [ 111 ], Lysobacter [ 112 ], Niveispirillum [ 113 ], Novosphingobium [ 114 ], Pandoraea [ 115 ], Polynucleobacter [ 83 ], Pseudomonas [ 98 ], Qipengyuania [ 116 ], Rhizobium [ 117 ], Stenotrophomonas [ 118 ], Taibaiella [ 119 ], Thermomonas [ 120 ], and Tsukamurella [ 121 ].…”

Section: Resultsmentioning

confidence: 99%

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

et al. 2022

View full text Add to dashboard Cite

The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating MMCs are not well known. Therefore, the main objective of this study was to address the shifts in the size and structure of the bacterial communities in two lab-scale sequencing batch reactors (SBRs) fed with fish-canning effluents and operated under non-saline (SBR-N, 0.5 g NaCl/L) or saline (SBR-S, 10 g NaCl/L) conditions, by using a combination of quantitative PCR and Illumina sequencing of bacterial 16S rRNA genes. A double growth limitation (DGL) strategy, in which nitrogen availability was limited and uncoupled to carbon addition, strongly modulated the relative abundances of the PHA-accumulating bacteria, leading to an increase in the accumulation of PHAs, independently of the saline conditions (average 9.04 wt% and 11.69 wt%, maximum yields 22.03 wt% and 26.33% SBR-N and SBR-S, respectively). On the other hand, no correlations were found among the PHAs accumulation yields and the absolute abundances of total Bacteria, which decreased through time in the SBR-N and did not present statistical differences in the SBR-S. Acinetobacter, Calothrix, Dyella, Flavobacterium, Novosphingobium, Qipengyuania, and Tsukamurella were key PHA-accumulating genera in both SBRs under the DGL strategy, which was revealed as a successful tool to obtain a PHA-enriched MMC using fish-canning effluents.

show abstract

“…In general, temperature is a very important parameter in controlling the richness and diversity of microorganisms in hot springs (Wang et al 2013 ). Several studies on microbial diversity in hot springs have also reported on the abundance and diversity of Bacillus species (Abdollahi et al 2021 ; Baltaci et al 2017 ; Ifandi and Alwi 2018 ; Margaryan et al 2018 ; Oztas Gulmus and Gormez 2020b ; Panosyan et al 2020 ). Although the locations of the Davut and Kopru hot springs are very close to each other, the isolated bacteria were quite different.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies on microbial diversity in hot springs have also reported on the abundance and diversity of Bacillus species (Abdollahi et al 2021;Baltaci et al 2017;Ifandi and Alwi 2018;Margaryan et al 2018;Oztas Gulmus and Gormez 2020b;Panosyan et al 2020) Microbial enzymes, especially enzymes derived from extremophile organisms, are widely used in different fields today. Thermophilic organisms are also microorganisms that adapt to extreme conditions, and so, they can create a variety of products that can be used even in unfavorable conditions.…”

Section: Discussionmentioning

confidence: 99%

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Uluçay

Görmez

Öziç

2022

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

In the last two decades, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring sources to explore the benefits of thermophiles in industrial and biotechnological fields. Within the scope of this study, a total of 83 thermophilic Bacilli strains were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 °C) located in the Eastern and Southeastern Anatolia Regions of Turkey. The physiological, morphological, biochemical and molecular properties of the isolates were determined. As a result of the 16S rRNA gene sequence analysis, 5 different species ( Bacillus licheniformis , Bacillus sp . , Bacillus subtilis , Geobacillus kaustophilus, and Weizmannia coagulans ,) were identified. B. licheniformis and B. subtilis were the most frequently encountered species among those obtained from the researched hot spring sources. Phylogenetic analysis was conducted to evaluate the phylogenetic relationships of the isolated species. The results showed that there was no significant difference between the groups and the bacteria in terms of the locations or optimum temperatures of the isolates. The bacterial isolates were screened for amylase, cellulase, lipase and protease hydrolytic enzyme activities. The hydrolytic enzyme production potentials among the isolates were identified in 68 (82%) isolates for amylase, 34 (41%) for cellulase, 69 (83%) for lipase and 73 (88%) for protease. All isolates were found to have at least one or more extracellular enzyme activities. Additionally, it was determined that 27 of the existing isolates (32.8%) were able to produce all of the aforementioned hydrolytic enzymes. Supplementary Information The online version contains supplementary material available at 10.1007/s10482-021-01678-5.

show abstract

Isolation and Characterization of Thermophilic Bacteria from Gavmesh Goli Hot Spring in Sabalan Geothermal Field, Iran: Thermomonas hydrothermalis and Bacillus altitudinis Isolates as a Potential Source of Thermostable Protease

Cited by 18 publications

References 26 publications

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Genome mining, phylogenetic, and functional analysis of arsenic (As) resistance operons in Bacillus strains, isolated from As-rich hot spring microbial mats

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Contact Info

Product

Resources

About