Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum)

Wadhwa, Zeenat; Srivastava, Vivek; Rani, Raj; vi, Tan; Makkar, Kanchan; Jangra, Sumit

doi:10.20546/ijcmas.2017.611.340

Cited by 15 publications

(14 citation statements)

References 10 publications

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“…sucrose, glucose and lactose as media for growth. After inoculation and incubation, colour were observed [2].…”

Section: Citrate Utilization Testmentioning

confidence: 99%

“…This experiment was carried out to identify sensitivity or resistance of local rhizobial isolates against different antibiotics were used to assay the antibiotic resistance on Millar-Hinton agar plates. Plates containing the discs were incubated at 28±2 °C for 3-7 days [2]. The presence or absence of inhibitory zones around different antibiotic discs was noted.…”

Section: Intrinsic Antibiotic Resistance (Iar) Spectramentioning

confidence: 99%

“…In agriculture, perhaps rhizobia-legume symbiosis contributes as much as 80 % of the biologically fixed nitrogen [2]. The bacteria of genera Rhizobium, Bradyrhizobium, Azorhizobium, Mesorhizobium and Ensifer, collectively called rhizobia, form symbiotic association with leguminous plants.…”

Section: Introductionmentioning

confidence: 99%

“…The salinity tolerance of sodium chloride salt was assayed by culturing the rhizobial bacteria on YEMA medium containing different sodium chloride salt concentration as follows: 1.0, 2.0, 3.0 and 4.0%, respectively [2].…”

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confidence: 99%

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Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Mohammed¹,

Sultan²

2021

J.Edu.Sci.

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In this study, thirty-six local isolates of rhizobial bacteria were isolated from root nodes of different leguminous plants collected from different areas in Nineveh Governorate/Iraq. The rhizobial colonies were purified and their agronomic and biochemical characteristics were studied. Results of this study showed that the local isolates had the ability to consume different sugars as a carbon source. Also, the antibiotic sensitivity test showed that only one isolate, Rhizobium leguminosarum bv. phaseoli AS12 was resistant to the antibiotic Azithromycin 15 µg/ml, and the isolate Rhizobium leguminosarum bv. phaseoli AS14 showed resistance to the antibiotic Gentamycin 10 µg/ml, and the rest of the isolates showed different rates of resistance and sensitivity for the rest of the antibiotics. The local isolates showed different NaCl tolerance levels up to 8.0 %, while no tolerance was observed for Rhizobium leguminosarum bv. viciae AS11 isolate at any of the studied concentrations. Results of heavy metal salt tolerance study showed that the isolates of Ensifer meliloti AS34 and Rhizobium leguminosarum bv. viciae AS36 were sensitive to ZnSo4 at a concentration of 25 µg/ml, while the tolerability of the other isolates to the studied heavy salts differed at concentrations higher than 25 µg /ml. All the studied isolates showed a positive result in staining with Congo red and Aniline blue dyes. Maximum exopolysaccharide production was 494 mg/Li by Rhizobium leguminosarum bv. viciae AS35. Antimicrobial activity test showed maximum inhibition zone 17 mm by Rhizobium leguminosarum bv. viciae AS36 against Proteus vulgaris.

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“…sucrose, glucose and lactose as media for growth. After inoculation and incubation, colour were observed [2].…”

Section: Citrate Utilization Testmentioning

confidence: 99%

Section: Intrinsic Antibiotic Resistance (Iar) Spectramentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Mohammed¹,

Sultan²

2021

J.Edu.Sci.

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“…Rhizobium bacteria are able to grow well in soil with neutral pH. Wadhwa et al (2017) said the optimum pH that supports rhizobial growth is about 6-7, while the minimum growth is about pH 8 and pH 9. Organism activities in the soil could be used as an indicators of soil quality.…”

Section: Resultsmentioning

confidence: 99%

Application of coconut shell charcoal and NPK fertilizer toward Acacia mangium growth on the soil of ex-limestone mining in Bogor, Indonesia

Wasis¹,

Ghaida²,

Winata³

2019

Arch. Agri. Environ. Sci.

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Limestone mining is an activity that can cause damage and negative impact on the environment. Revegetation is needed to be done so that the damaged land condition can be returned in accordance with its designation. Acacia mangium is one of the species that can be used in revegetation of mining area. This study aims to increase the A. mangium growth response at the limestone mining land that can be used as a tool for consideration in revegetation activities. The design used in this experiment was a complete randomized design (CRD) factorial with 2 factors. The first factor was the application of coconut shell charcoal and the second factor was the application of NPK fertilizer. The results showed that the addition of coconut shell charcoal could give effect to the number of nodules with the best dose of 90 g and NPK fertilizer was able to give effect to the growth of diameter and the number of root nodules with the best dose of 15 g.

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Soil Salinity and Climate Change: Microbiome-Based Strategies for Mitigation of Salt Stress to Sustainable Agriculture

Phour

Sindhu

2023

Climate Change Management

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Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum)

Cited by 15 publications

References 10 publications

Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Isolation and Characterization of Local Isolates of Rhizobia in Nineveh Governorate/Iraq

Application of coconut shell charcoal and NPK fertilizer toward Acacia mangium growth on the soil of ex-limestone mining in Bogor, Indonesia

Soil Salinity and Climate Change: Microbiome-Based Strategies for Mitigation of Salt Stress to Sustainable Agriculture

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