Isolation and Characterization of Novel Lignolytic, Cellulolytic, and Hemicellulolytic Bacteria from Wood-Feeding Termite Cryptotermes brevis

Tsegaye, Bahiru; Balomajumder, Chandrajit; Roy, Partha

doi:10.1007/s10123-018-0024-z

Cited by 64 publications

(30 citation statements)

References 46 publications

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“…Metagenomics allows retrieval of genes encoding biocatalysts from culturable as well as non-cultural microbes. Many cellulase genes have been identified from environmental metagenomes ( Garg et al, 2016 ; Matsuzawa and Yaoi, 2016 ; Pandey et al, 2016 ; Zhou et al, 2016 ), hindgut contents of termites ( Duan et al, 2017 ; Tsegaye et al, 2018 ), rumen ( Ko et al, 2015 ; Song et al, 2016 ; Do et al, 2018 ), compost ( Lemos et al, 2017 ; Lee et al, 2018 ), oil reservoirs ( Lewin et al, 2017 ), and sludge from a biogas reactor ( Wei et al, 2015 ). Very few of them have, however, been characterized adequately ( Garg et al, 2016 ; Song et al, 2016 ; Lee et al, 2018 ).…”

Section: Cellulases Retrieved From Metagenomesmentioning

confidence: 99%

Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

2020

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Lignocellulosic biomass is a renewable and sustainable energy source. Cellulases are the enzymes that cleave β-1, 4-glycosidic linkages in cellulose to liberate sugars that can be fermented to ethanol, butanol, and other products. Low enzyme activity and yield, and thermostability are, however, some of the limitations posing hurdles in saccharification of lignocellulosic residues. Recent advancements in synthetic and systems biology have generated immense interest in metabolic and genetic engineering that has led to the development of sustainable technology for saccharification of lignocellulosics in the last couple of decades. There have been several attempts in applying genetic engineering in the production of a repertoire of cellulases at a low cost with a high biomass saccharification. A diverse range of cellulases are produced by different microbes, some of which are being engineered to evolve robust cellulases. This review summarizes various successful genetic engineering strategies employed for improving cellulase kinetics and cellulolytic efficiency.

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Section: Cellulases Retrieved From Metagenomesmentioning

confidence: 99%

Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

2020

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“…Hydrolytic microorganisms can have a capability to establish synergistic relationships with each other to degrade polysaccharides in lignocellulosic biomass. Cellulase and xylanase are the best examples for the complete degradation of cellulose and hemicellulose in biomass (Tsegaye et al 2018a(Tsegaye et al , 2018b. Cellulase is responsible for decomposing cellulose into its constituents, and xylanase decomposes hemicellulose.…”

Section: Hydrolysis Of Polysaccharides In Lignocellulose Biomassmentioning

confidence: 99%

“…Biological pretreatments are usually performed by employing lignolytic and cellulolytic microorganisms, which synthesize potent lignolytic and cellulolytic enzymes during hydrolysis (Ruqayyah et al 2013;Kadri et al 2016;Neamah et al 2016). The efficiency of biological delignification depends upon the type of microorganism used, nature and type of substrate used, and the cultivation methods and conditions used (Liu et al 2017;Narra et al 2017;Tsegaye et al 2018a, b). Bioethanol can be acquired from lignocellulose biomass after successive processes including pretreatments to remove lignin and releases the entangled polysaccharides and fermentation (Silveira et al 2015;Oonkhanond et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect

2019

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Background: The depletion of fossil fuel and its huge environmental problem are currently a concern for a scientific community in the area of energy engineering. This opened research opportunities for searching alternate renewable energy sources especially biofuel production from lignocellulose biomass resources. The main objective of this paper is to review the delignification and hydrolysis capabilities of microorganisms (bacteria and fungi). Results: Currently, different types of lignocellulose biomass pretreatment technologies are available. All of the technologies are either in lab scale or in pilot scale. Among the pretreatment technologies, biological pretreatments attract many attentions because of their eco-friendly advantages, performed at a mild temperature and do not produce inhibitory compounds during the pretreatment process. Industrial-scale biofuel production using biological delignification and hydrolysis process is still at lab scale, and intensive research works are required. The cost of biofuel production from lignocellulose biomass is currently expensive. Conclusion: Searching for the best microbial strains having efficient lignin-degrading and polysaccharide-hydrolyzing capabilities is vital to realize industrial-scale biofuel production from lignocellulose biomass. Process optimization along with genetic engineering of microorganisms is seen as a potential for biofuel production from lignocellulose biomass.

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“…Hydrolytic bacteria have been isolated from termite gut and used for hydrolysis of pretreated rice straw. Details of the isolation and characterization of the isolates were mentioned in our previous work (Tsegaye et al 2019b).…”

Section: Isolation Of Hydrolytic Bacteria From a Termitementioning

confidence: 99%

Alkali delignification and Bacillus sp. BMP01 hydrolysis of rice straw for enhancing biofuel yields

2019

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Background: Lignocellulose biomass such as agricultural residues is increasingly important for biofuel production. Using agricultural residues like rice straw for biofuel production has dual purposes of utilizing the huge waste generated. The abundant availability and high polysaccharide contents are the main reason for biofuel production. However, the use is limited due to the reluctant nature of lignin in the biomass. Therefore, sodium hydroxide was used for solubilizing lignin and preserving the polysaccharides. Results: About 71.29% of lignin (maximum amount of lignin) was removed after 7% sodium hydroxide (NaOH) pretreatment followed by 3% pretreatment (about 67.13%). Also, maximum cellulose (about 71.33%) was preserved after 7% NaOH pretreatment. Results of reducing sugars obtained by spectrophotometric analysis (3,5-dinitrosalycilic acid method) of pretreated rice straw show about 88.27% conversion (753 mg/g) after the 13th day of hydrolysis by bacteria isolated from a termite. FE-SEM, XRD, FTIR, and TGA analyses show a significant amount of lignin was removed which helps in releasing cellulose tangled in lignin. Conclusion: Rice straw pretreatment at 7% NaOH and hydrolysis by Bacillus sp. BMP01 achieved the release of a high yield of fermentable sugars that increased the yield of bioethanol. This study demonstrates the potential of alkali pretreatment coupled with microbial hydrolysis for efficient bioethanol production from agricultural residues like rice straw.

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Isolation and Characterization of Novel Lignolytic, Cellulolytic, and Hemicellulolytic Bacteria from Wood-Feeding Termite Cryptotermes brevis

Cited by 64 publications

References 46 publications

Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect

Alkali delignification and Bacillus sp. BMP01 hydrolysis of rice straw for enhancing biofuel yields

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