2017
DOI: 10.1099/ijsem.0.001957
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Abstract: We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evid… Show more

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Cited by 34 publications
(55 citation statements)
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“…Nevertheless, extended analysis unveiled that this K. kingae RTX locus is flanked by insertion elements and possesses a reduced GC content, strongly suggesting acquisition by horizontal gene transfer (23). However, the type strain K. negevensis Sch538, which shares close phenotypic and genomic relatedness with K. kingae (6), harbors an identical Kingella RTX locus. Because the Kingella RTX locus was previously considered to be restricted to K. kingae, this raised the question of whether qPCRs targeting rtxA, rtxB, and groEL, which are in widespread use for the diagnosis of K. kingae infection, can differentiate K. kingae from K. negevensis.…”
mentioning
confidence: 99%
“…Nevertheless, extended analysis unveiled that this K. kingae RTX locus is flanked by insertion elements and possesses a reduced GC content, strongly suggesting acquisition by horizontal gene transfer (23). However, the type strain K. negevensis Sch538, which shares close phenotypic and genomic relatedness with K. kingae (6), harbors an identical Kingella RTX locus. Because the Kingella RTX locus was previously considered to be restricted to K. kingae, this raised the question of whether qPCRs targeting rtxA, rtxB, and groEL, which are in widespread use for the diagnosis of K. kingae infection, can differentiate K. kingae from K. negevensis.…”
mentioning
confidence: 99%
“…Kingella negevensis is a newly described Gram-negative bacterium belonging to the genus Kingella in the Neisseriaceae family (1). Asymptomatically carried in the oropharynx of young children, most K. negevensis strains have been isolated in southern Israel to date (1).…”
Section: Genome Announcementmentioning
confidence: 99%
“…Asymptomatically carried in the oropharynx of young children, most K. negevensis strains have been isolated in southern Israel to date (1). To the best of our knowledge, no genome sequence from European strains of this novel Kingella sp.…”
Section: Genome Announcementmentioning
confidence: 99%
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