Isolation and characterization of indigenous copper-resistant actinomycete strains

Albarracín, Virginia Helena; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio

doi:10.1016/j.chemer.2005.06.004

Cited by 74 publications

(70 citation statements)

References 17 publications

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“…Recent progress has been made studying metal resistance in actinobacteria isolated from polluted areas [4 -12]. However, copper resistance in actinobacteria has been little studied [7,9,11,13]. In previous works, the reported copper resistance levels for actinobacteria were obtained by testing in agar complex media [4 -5, 14] but, in this kind of assays, metal is not completely available to the microorganisms which could lead to assume erroneous, high levels of resistance.…”

Section: Introduction *mentioning

confidence: 98%

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Albarracín

Winik

Kothe

et al. 2008

J. Basic Microbiol.

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Amycolatopsis sp. AB0, a copper resistant actinobacterium isolated from polluted sediments, has shown high copper specific biopsortion ability (25 mg g(-1)). Two approaches were used to confirm metal accumulation in growing cells of Amycolatopsis sp. AB0; we performed subcellular fractioning assays which showed that the retained copper was associated with the extra-cellular fraction (exopolymer, 40%), but mainly within the cells. Intracellular distribution of copper was: 86% in the cytosolic fraction, 11% at the cell wall and 3% associated with the ribosome/membrane fraction. Its copper bioaccumulation ability was corroborated by using silver enhanced staining of copper with the Timm's reagent technique, which has not been used to detect metal deposits in bacteria before. In addition, we constructed specific oligonucleotides for targeting genes coding for copper P-Type ATPases that could be involved in the copper uptake ability of this strain. A 607 bp DNA fragment was amplified and sequenced from Amycolatopsis sp AB0. BLAST search analysis showed 71% protein homology of the deduced sequence with a putative cation-transporting ATPase of Nocardia farcinica and 65% with a copper translocating ATPase of Mycobacterium flavescens. To our knowledge this is the first report of the presence of copper P-type ATPase genes in the Amycolotopsis genus.

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Section: Introduction *mentioning

confidence: 98%

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Albarracín

Winik

Kothe

et al. 2008

J. Basic Microbiol.

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“…Albarracin et al (2005) isolated actinomycetes from a copper-contaminated area. Polti (2007) characterized and identified some of chromium-(VI)-resistant actinomycetes strains as species of the genera Streptomyces and Amycolatopsis.…”

Section: Introductionmentioning

confidence: 99%

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

et al. 2014

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Heavy metals are a significant source of pollution in soils that have been demonstrated to exert significant toxic effect on soil microbial assemblages. Here we investigate the occurrence and metabolic characteristics of actinobacteria, which form a predominated component of farmland bacterial community near the town of Sereď in southwest Slovakia, contaminated by close nickel ore facility. Actinobacteria occurred in this environment with high concentrations of nickel (2.109 mg/kg), slightly above the natural occurrence of cobalt (355 mg/kg) and zinc (177 mg/kg), even too low concentration of iron (35.75 mg/kg) for a normal soil and not a toxic amount of copper (32.2 mg/kg) and cadmium (<0.25 mg/kg). The phylogeny was reconstructed using partial sequences of 16S rDNA genes of both, actinobacterial isolates and clones. A total of 105 actinobacterial representatives were divided into 66 species belonging to one order, 7 genera and 5 uncategorized groups. The selected 14 morphologically distinct isolates were able to produce drop collapsing, haemolytic and lipase activities. Whereas only 4 isolates produced dark brown melanin pigment and only 5 of them produced decolourization of the azo dye, all isolates tested were capable of assimilating all 11 sugars tested. All these actinobacterial isolates were resistant to nickel, cobalt, zinc, cadmium, copper, but the level of resistance differed between the individual isolates, and the resistance profiles of antibiotics (gentamycin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, penicillin-G) varied among them to a certain extent. Our results suggested that actinobacteria in soil contaminated by nickel present a relatively divergent group inside of microbial assemblage.

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“…In particular, several studies have indicated their potential for the remediation of heavy metals [55,56,57,58,59]. Specifically, Verma & Singh [60], found that Brevibacterium casie was capable of reducing 78% Cr 6+ and 82% polychlorinated biphenyls in an LB broth medium, indicating that these strain may have the potential to be used in bioremed iation.…”

Section: S Rrna Gene Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Bioremediation of Landfill Leachate Using Isolated Bacterial Strains

Morris¹,

Garcia-Cabellos²,

Enright³

et al. 2018

IJEBB

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Landfilling is one of the most common and widely accepted practices for the disposal of waste throughout the world. Leachate, a major drawback of landfilling, continues to be produced at vast rates and current treatment options are costly and often inadequate. The management of leachate is of economic and environmental importance, due to its potential to cause contamination to ground and surface water. This research focuses on treating leachate in a cost-effective manner through bioremediation. Microorganisms were isolated from landfill leachate (LFL) and screened to determine their ability to remediate a wide range of compounds found in leachate, such as ammonia, phosphate and nitrate. Selected isolates were identified as belonging to the phylum"s Firmicutes, Actinobacteria, and Proteobacteria, isolates were inoculated into soil contained in a fixed bed column system. The column system was optimised and used for the treatment of LFL over a 10-hour period. High percentage removal rates were achieved for ammonia (>90%) and removal nitrate and phosphate (>60%). Although EPA discharge limits were not achieved, bioremediation using selected microbial strains represents a cost effective treatment option when compared to conventional methods. Research is now required to further optimise this system to achieve discharge limits for all compounds tested.

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Isolation and characterization of indigenous copper-resistant actinomycete strains

Cited by 74 publications

References 17 publications

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

Bioremediation of Landfill Leachate Using Isolated Bacterial Strains

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