Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Iwatani, Sota; Yoshida, Makiko; Yamana, Kazunari; Kurokawa, Daisuke; Kuroda, Jumpei; Thwin, Khin Kyae Mon; Uemura, Suguru; Takafuji, Satoru; Nino, Nanako; Koda, Tsubasa; Mizobuchi, Masami; Nishiyama, Masahiro; Fujioka, Kazumichi; Nagase, Hiroaki; Morioka, Ichiro; Iijima, Kazumoto; Nishimura, Noriyuki

doi:10.3791/58806-v

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human preterm- and term-UC-MSCs were isolated and cultured as described previously [ 39 , 41 , 42 ]. Briefly, preterm- and term-UC (2–3 g wet weight) were collected, cut into 2–3 mm 3 pieces, enzymatically dissociated with Liberase DH Research Grade (Roche, Mannheim, Germany), and filtered through a 100 µm cell strainer (BD Bioscience, Bedford, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Human Muse cells isolated from preterm- and term-umbilical cord delivered therapeutic effects in rat bleomycin-induced lung injury model without immunosuppressant

Win,

Kushida,

Yamana

et al. 2024

Stem Cell Res Ther

Self Cite

View full text Add to dashboard Cite

Background Bleomycin (BLM)-induced lung injury is characterized by mixed histopathologic changes with inflammation and fibrosis, such as observed in human patients with bronchopulmonary dysplasia, idiopathic pulmonary fibrosis, and chronic obstructive pulmonary disease. Although no curative therapies for these lung diseases exist, stem cell therapy has emerged as a potential therapeutic option. Multilineage-differentiating stress-enduring (Muse) cells are endogenous pluripotent- and macrophage-like stem cells distributed in various adult and fetal tissues as stage-specific embryonic antigen-3-positive cells. They selectively home to damaged tissue by sensing sphingosine-1-phosphate and replace the damaged/apoptotic cells by in vivo differentiation. Clinical trials for some human diseases suggest the safety and therapeutic efficacy of intravenously injected human leukocyte antigen-mismatched allogenic Muse cells from adult bone marrow (BM) without immunosuppressant. Here, we evaluated the therapeutic effects of human Muse cells from preterm and term umbilical cord (UC), and adult BM in a rat BLM-induced lung injury model. Methods Rats were endotracheally administered BLM to induce lung injury on day 0. On day 3, human preterm UC-Muse, term UC-Muse, or adult BM-Muse cells were administered intravenously without immunosuppressants, and rats were subjected to histopathologic analysis on day 21. Body weight, serum surfactant protein D (SP-D) levels, and oxygen saturation (SpO2) were monitored. Histopathologic lung injury scoring by the Ashcroft and modified American Thoracic Society document scales, quantitative characterization of engrafted Muse cells, RNA sequencing analysis, and in vitro migration assay of infused Muse cells were performed. Results Rats administered preterm- and term-UC-Muse cells exhibited a significantly better recovery based on weight loss, serum SP-D levels, SpO2, and histopathologic lung injury scores, and a significantly higher rate of both Muse cell homing to the lung and alveolar marker expression (podoplanin and prosurfactant protein-C) than rats administered BM-Muse cells. Rats receiving preterm-UC-Muse cells showed statistically superior results to those receiving term-UC-Muse cells in many of the measures. These findings are thought to be due to higher expression of genes related to cell migration, lung differentiation, and cell adhesion. Conclusion Preterm UC-Muse cells deliver more efficient therapeutic effects than term UC- and BM-Muse cells for treating BLM-induced lung injury in a rat model.

show abstract

Section: Methodsmentioning

confidence: 99%

Human Muse cells isolated from preterm- and term-umbilical cord delivered therapeutic effects in rat bleomycin-induced lung injury model without immunosuppressant

Win,

Kushida,

Yamana

et al. 2024

Stem Cell Res Ther

Self Cite

View full text Add to dashboard Cite

show abstract

“…UC MSCs express similar markers, namely CD73, CD90, CD105, CD106 and embryonic stem cell markers (Sex-determining region Y-box 2 (SOX2) and stage-specific embryonic antigen-4 (SSEA4)). 60,62,63 MSCs derived from five discrete zones of placenta exhibit similar immunogenic phenotypes and differentiation capabilities, but their distinctive features are as follows:…”

Section: Distinctive Source-dependent Features Of Placental Mscsmentioning

confidence: 99%

“…The removal of nucleic acids (DNA and RNA), cell membrane receptors, cellular proteins and damage-associated molecular pattern molecules (DAMPs) along with cellular debris is imperative for an ideally decellularized ECM. 46,63 DNA/RNA remnants in decellularized matrix prove to be detrimental for tissue engineering applications due to their immunogenic reaction when in contact with host tissue. The presence of DNA is detected in biological samples by means of qualitative assays, namely histological staining.…”

Section: Biomaterials Science Reviewmentioning

confidence: 99%

Human placenta/umbilical cord derivatives in regenerative medicine – Prospects and challenges

et al. 2023

View full text Add to dashboard Cite

show abstract

Mesenchymal stem cell conditioned medium attenuates oxidative stress injury in hepatocytes partly by regulating the miR-486-5p/PIM1 axis and the TGF-β/Smad pathway

Lin

et al. 2021

Bioengineered

View full text Add to dashboard Cite

show abstract

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Cited by 3 publications

References 0 publications

Human Muse cells isolated from preterm- and term-umbilical cord delivered therapeutic effects in rat bleomycin-induced lung injury model without immunosuppressant

Human Muse cells isolated from preterm- and term-umbilical cord delivered therapeutic effects in rat bleomycin-induced lung injury model without immunosuppressant

Human placenta/umbilical cord derivatives in regenerative medicine – Prospects and challenges

Mesenchymal stem cell conditioned medium attenuates oxidative stress injury in hepatocytes partly by regulating the miR-486-5p/PIM1 axis and the TGF-β/Smad pathway

Contact Info

Product

Resources

About