Isolation and characterization of heparan sulfate from various murine tissues

Abstract

Heparan sulfate (HS), is a proteoglycan (PG) found both in the extracellular matrix and on cell surface. It may represent one of the most biologically important glycoconjugates, playing an essential role in a variety of different events at molecular level. The publication of the mouse genome, and the intensive investigations aimed at understanding the proteome it encodes, has motivated us to initiate studies in mouse glycomics focused on HS. The current study is aimed at determining the quantitative and qualitative organ distribution of HS in mice. HS from brain, eyes, heart, lung, liver, kidney, spleen, intestine and skin was purified from 6-8 week old male and female mice. The recovered yield of HS from these organs is compared with the recovered whole body yield of HS. Structural characterization of the resulting HS relied on disaccharide analysis and 1 H-NMR spectroscopy. Different organs revealed a characteristic HS structure. These data begin to provide a structural understanding of the role of HS in cell-cell interactions, cell signaling

Organ-specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice

“…The percentages were relatively high (Ͼ10%) for ⌬UA2S-GlcNS6S in the liver (16.7%) and kidney (10.7%), for ⌬UA-GlcNS6S in the spleen (14.9%) and kidney (10.7%), for ⌬UA2S-GlcNS in the lung (17.2%), brain (14.7%), and testis (13.7%), and for ⌬UA-GlcNAc6S in the spleen (12.6%), kidney (12.2%), and liver (10.1%). These sulfation profiles of HS concur well with those previously reported in mouse and bovine organs (37)(38)(39)(40).…”

Quantitative and Qualitative Alterations of Heparan Sulfate in Fibrogenic Liver Diseases and Hepatocellular Cancer

“…This schedule was also designed to minimize the opportunity for any possible postextraction modifications to the integrity and sulfation patterns of the HS due to endogenous enzyme activities (i.e, Sulfs and HPSE). Table 2 summarizes a comparison of disaccharide compositions obtained in this study with those in literature data for healthy human, rat, mouse, and porcine liver HS (Toida et al 1997;Vongchan et al 2005;Warda et al 2006;Deakin and Lyon 2008). In our analyses, a markedly lower overall sulfation, primarily due to a relatively low content of trisulfated disaccharide, was observed in all the samples when compared with previously published analyses of healthy liver HS of human and other species.…”

Extended N-Sulfated Domains Reside at the Nonreducing End of Heparan Sulfate Chains

“…Intact HS chains were recovered by ion-exchange chromatography. Murine organ HS preparations were the generous gift of Dr. Fuming Zhang and Dr. Robert Linhardt and were isolated as described previously (22). Briefly, tissues were minced, homogenized, and defatted.…”