Isolation and Characterization of Enzymes Involved in Lysine Catabolism from Sorghum Seeds

Fornazier, Ricardo Francisco; Gaziola, Salete Aparecida; Helm, Cristiane Vieira; Lea, Peter J.; Azevedo, Ricardo Antunes

doi:10.1021/jf048525o

Cited by 15 publications

(8 citation statements)

References 43 publications

(94 reference statements)

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“…Fornazier et al (2005) using infrared analysis, compared MASSA 03 to some of the sorghum high-lysine genotypes also used in this study, and observed a lysine concentration slightly lower than the high-lysine genotypes indicating that such a genotype could eventually be also considered a sorghum genotype with higher lysine content. Furthermore, analysis of enzymes involved in lysine degradation in the seeds of MASSA 03 also suggested a different lysine metabolic regulation since the enzymes involved in lysine degradation in the seeds had a drastic reduction in activity when compared to maize wild-type lines.…”

Section: Amino Acid Analysismentioning

confidence: 86%

“…Cereal grains generally contain low levels of lysine , while legumes are normally deficient in methionine . Due to nutritional importance of these amino acids, the aspartate pathway has received special attention since the early 1970s, and a considerable amount of information has been obtained about the metabolic control of these amino acids (Gaziola et al, 1997;Arruda et al, 2000;Fornazier et al, 2005;Ferreira et al, 2006;Hudson et al, 2006;Nikiforova et al, 2006;Stepansky et al, 2006;Wang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Nutritional Quality of Sorghum Seeds: Storage Proteins and Amino Acids

et al. 2008

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One sorghum commercial genotype (MASSA 03) and nine ICRISAT high-lysine genotypes from India were analyzed for storage protein content, distribution profile, and soluble amino acid concentrations. Storage proteins fraction were extracted and separated by SDS-PAGE. Soluble amino acids contents were determined by HPLC. Variations in intensity and appearance and disappearance of protein bands were observed among the sorghum genotypes suggesting genetic variability. Amino acid profile also indicated large variations in the amino acid concentrations. The high lysine and threonine soluble concentrations observed in the seeds of the sorghum genotypes encouraged the use of these genotypes as potential food source due to the better balanced amino acids profile.

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Section: Amino Acid Analysismentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Nutritional Quality of Sorghum Seeds: Storage Proteins and Amino Acids

et al. 2008

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“…New insights into the regulation of lysine biosynthesis and catabolism were reviewed by Galili (2002). The potential for manipulating lysine accumulation will also increase as more genes and enzymes involved in lysine synthesis and catabolism are isolated and characterised, such as reported recently for two enzymes (Fornazier et al 2005).…”

Section: Transgenic Cereal Storage Protein Genesmentioning

confidence: 99%

Multifunctional grains for the future: genetic engineering for enhanced and novel cereal quality

Godwin

Williams

Pandit

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Cereals provide more than half the world population's calorific intake, and have a variety of other important uses as food and beverage ingredients, livestock feeds, and as sources of renewable energy and industrial components. The technology to genetically modify many important cereals is now well-established, thereby presenting new opportunities to produce cereals with enhanced quality and novel properties. In 2007, GM (genetically modified) maize with insect and herbicide resistance was grown on over 30 million hectares worldwide, yet to date, there are no GM cereals with enhanced or novel grain (enduse) qualities being grown in commercial farmers' fields. This review will discuss some of the latest GM technology developments reported to enhance the quality of cereals for food and other uses. Developments and opportunities involving gene manipulation for starch and protein quality, as well as non-starch polysaccharides, phenolic compounds and micronutrients will also be discussed. The current paucity of GM cereals with enhanced grain quality is not related to the absence of technological progress, rather it is the regulatory and consumer acceptance issues that have slowed the release of these crops.

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“…These questions are also valid for other cereal crops such as rice, barley and sorghum, for which high-lysine mutants (e.g., high-lysine sorghum mutants, hiproly barley mutant, high-lysine rice mutants) have been available for some time (18,59,60). Very little is known about the aspartate metabolic pathway and, more specifically, lysine metabolism in the mutants of crops other than maize.…”

Section: Opaque-2 and Quality Protein Maizementioning

confidence: 99%

Are high-lysine cereal crops still a challenge?

Ferreira

Varisi

Meinhardt

et al. 2005

Braz J Med Biol Res

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The essential amino acids lysine and threonine are synthesized in higher plants via a pathway starting with aspartate that also leads to the formation of methionine and isoleucine. Lysine is one of most limiting amino acids in plants consumed by humans and livestock. Recent genetic, molecular, and biochemical evidence suggests that lysine synthesis and catabolism are regulated by complex mechanisms. Early kinetic studies utilizing mutants and transgenic plants that overaccumulate lysine have indicated that the major step for the regulation of lysine biosynthesis is at the enzyme dihydrodipicolinate synthase. Despite this tight regulation, recent strong evidence indicates that lysine catabolism is also subject to control, particularly in cereal seeds. The challenge of producing crops with a high-lysine concentration in the seeds appeared to be in sight a few years ago. However, apart from the quality protein maize lines currently commercially available, the release of high-lysine crops has not yet occurred. We are left with the question, is the production of high-lysine crops still a challenge?

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Isolation and Characterization of Enzymes Involved in Lysine Catabolism from Sorghum Seeds

Cited by 15 publications

References 43 publications

Nutritional Quality of Sorghum Seeds: Storage Proteins and Amino Acids

Nutritional Quality of Sorghum Seeds: Storage Proteins and Amino Acids

Multifunctional grains for the future: genetic engineering for enhanced and novel cereal quality

Are high-lysine cereal crops still a challenge?

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