Isolation and Characterization of Endophytic Bacteria from the Nickel Hyperaccumulator Plant Alyssum bertolonii

Barzanti, R.; Ozino, Francesca; Bazzicalupo, Marco; Gabbrielli, R.; Galardi, Francesca; Gonnelli, Cristina; Mengoni, Alessio

doi:10.1007/s00248-006-9164-3

Cited by 190 publications

(92 citation statements)

References 45 publications

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“…Moreover, in Thlaspi (Idris et al 2004), more isolates tolerant to higher Ni concentration were found in the plant endosphere than in the rhizosphere, thus reinforcing the hypothesis that Ni-hyperaccumulating plants provide highly selective and peculiar niches for bacteria. In agreement with this finding, endospheres from roots, shoots and leaves of A. bertolonii were shown to contain bacterial isolates possessing different levels of Niresistance (Barzanti et al 2007) although the biological reasons for such differences is elusive yet. Notably, isolates from leaves displayed the lowest levels of Niresistance compared with those from the other organs in agreement with a low Ni bioavailability due to vacuolar storage of Ni in epidermal cells and trichomes (Ingle et al 2008;Kupper et al 2001;Smart et al 2007).…”

Section: Bacterial Communities In Serpentine Soilmentioning

confidence: 53%

Plants as extreme environments? Ni-resistant bacteria and Ni-hyperaccumulators of serpentine flora

2009

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During recent years there has been an increasing interest in the bacterial communities occurring in unusual, often extreme, environments. On serpentine outcrops around the world, a high diversity of plant species showing the peculiar features of metal hyperaccumulation is present. These metal hyperaccumulators have received much attention for their potential biotechnological exploitation in phytoremediation processes, but also as unusual, extreme habitats for the associated bacterial flora, which could reveal novel details concerning bacterial adaptation. This paper will briefly focus on the research topics that have been addressed to date on bacteria associated with serpentine plants and aims to provide a state of the art and to present possible future directions for research which could lead to new insights on microbial adaptation and evolution, and potentially applied in technologies for sustainable use and remediation of contaminated land.

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Section: Bacterial Communities In Serpentine Soilmentioning

confidence: 53%

Plants as extreme environments? Ni-resistant bacteria and Ni-hyperaccumulators of serpentine flora

2009

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“…At present, however, little evidence is available concerning the effect of metal hyperaccumulation and metal-based defences upon the endophytic bacteria of hyperaccumulator plants. Two studies have surveyed the endophytes associated with nickel hyperaccumulator plants on natural serpentine soils [19,20]. Both reported that these bacteria showed high nickel tolerance, supporting the notion that the plants provide a high-metal environment for bacteria.…”

Section: Introductionmentioning

confidence: 90%

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

et al. 2016

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Metal-hyperaccumulating plants, which are hypothesized to use metals for defence against pests and pathogens, provide a unique context in which to study plant -pathogen coevolution. Previously, we demonstrated that the high concentrations of zinc found in leaves of the hyperaccumulator Noccaea caerulescens provide protection against bacterial pathogens, with a potential trade-off between metal-based and pathogen-induced defences. We speculated that an evolutionary arms race between zinc-based defences in N. caerulescens and zinc tolerance in pathogens might have driven the development of the hyperaccumulation phenotype. Here, we investigate the possibility of local adaptation by bacteria to the zinc-rich environment of N. caerulescens leaves and show that leaves sampled from the contaminated surroundings of a former mine site harboured endophytes with greater zinc tolerance than those within plants of an artificially created hyperaccumulating population. Experimental manipulation of zinc concentrations in plants of this artificial population influenced the zinc tolerance of recovered endophytes. In laboratory experiments, only endophytic bacteria isolated from plants of the natural population were able to grow to high population densities in any N. caerulescens plants. These findings suggest that long-term coexistence with zinc-hyperaccumulating plants leads to local adaptation by endophytic bacteria to the environment within their leaves.

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“…Plant samples (leaves, stems, roots, flowers, fruits) were washed thoroughly with tap water followed by sterile double distilled water and drained before immersion in 70% ethyl alcohol for 30s and sodium hypochlorite solution (2%) for 2min. The plant parts were then immersed in 70% ethyl alcohol for 30s (Barzanti et al 2007) and then rinsed with sterile double distilled water. Each plant part was cut aseptically into 1cm long segments using a sterile blade under the laminar flow hood and allowed to dry.…”

Section: Plant Sample Collectionmentioning

confidence: 99%

Epiphytic and endophytic bacteria that promote growth of ethnomedicinal plants in the subtropical forests of Meghalaya, India

Nongkhlaw

Joshi

2014

RBT

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The present study was aimed to investigate the endophytic and epiphytic bacteria associated with selected ethnomedicinal plants from the pristine subtropical forests of Meghalaya and analyse them for plant growth promotion and antagonistic ability. This study is an attempt to explore plant associated bacteria which are beneficial to host plants, and thus aid in the conservation of ethnomedicinal plants of the studied subtropical forests, which are dwindling due to exploitation. The plant growth promotion parameters like indole acetic acid (IAA) production, mineral phosphate solubilisation, acid phosphatase activity, presence of 1-aminocyclopropane-1-carboxylic acid deaminase (ACC) gene, nitrogen fixation, cellulose digestion, chitin and pectin degradation were screened among the isolates. The study revealed significant differences in bacterial population not only between the epiphytic and endophytic microhabitats, but also amongst the host plants. Out of the 70 isolated plant associated bacteria, Bacillus sp., Serratia sp., Pseudomonas sp., Pantoea sp., and Lysinibacillus sp. showed potent plant growth promotion properties. Bacillus siamensis C53 and B. subtilis cenB showed significant antagonistic activity against the tested pathogens. This study indicated the isolates inhabiting the plants prevalent in the subtropical sacred forests could be explored for use as plant growth promoters while practising the cultivation and conservation of ethnomedicinal plants. Rev. Biol. Trop. 62 (4): 1295-1308. Epub 2014 December 01.

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Isolation and Characterization of Endophytic Bacteria from the Nickel Hyperaccumulator Plant Alyssum bertolonii

Cited by 190 publications

References 45 publications

Plants as extreme environments? Ni-resistant bacteria and Ni-hyperaccumulators of serpentine flora

Plants as extreme environments? Ni-resistant bacteria and Ni-hyperaccumulators of serpentine flora

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

Epiphytic and endophytic bacteria that promote growth of ethnomedicinal plants in the subtropical forests of Meghalaya, India

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