Isolation and Characterization of Cold Active Bacterial Species from Municipal Solid Waste Landfill Site

Hamid, Basharat; Jehangir, Arshid; Baba, Zahoor Ahmad; Fatima, Sabah

doi:10.3923/rjes.2019.1.9

Cited by 11 publications

(3 citation statements)

References 15 publications

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“…et al, 2012). Another study revealed an average cfu/g was 3.7×10 6 cfu/g at the temperature of 15°C (Hamid et al, 2019).…”

Section: Isolation Of Microbes From Municipal Solid Wastementioning

confidence: 96%

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Afshan,

Ghouri,

Hayat

et al. 2023

Acta Bras.

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Solid waste dump site-based pollution is one of the colossal health concerns globally due to presence of cellulose, starch, protein, and lipids, which is a perfect nutrient waste for the development of biodegrading potential microbes. The current study aimed to determine the productive microorganisms for waste biodegradation. Ten samples were collected from different areas of Karachi waste dump sites. The identification and characterization of isolates were done by morphological and biochemical tests. The isolated microbes were qualitatively screened for the action of making industrially key bio-enzymes. The potential enzymes producing microbes were subjected to waste degradation test for 90 days. Changes of odor, color and weight loss of decomposing garbage were noted. The highest degradation ability in term of weight loss was shown by Aspergillus (SSI14) and Bacillus (SSI6). Four metals (zinc sulphate, cadmium chloride, copper sulphate and iron sulphate) were chosen for the purpose of metals tolerance test and minimum inhibitory concentration (MIC) of the isolated microbial strains. Current study reported that Zn was found to be more lethal in in comparison to Cu and Fe. Molecular identification of the isolated microbes was done by polymerase chain reaction. The microbial strains were characterized by the polymerase chain reaction (PCR) followed by confirmation of product by agarose gel electrophoresis. The findings can be served as baseline data to develop microbial apparatus for biodegradation and management of solid waste.

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“…et al, 2012). Another study revealed an average cfu/g was 3.7×10 6 cfu/g at the temperature of 15°C (Hamid et al, 2019).…”

Section: Isolation Of Microbes From Municipal Solid Wastementioning

confidence: 96%

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Afshan,

Ghouri,

Hayat

et al. 2023

Acta Bras.

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“…Min journal.ump.edu.my/cst ◄ the findings from Hamid et al[28]. It can be assumed that Bacteria 10 might be Staphylococci sp.…”

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confidence: 92%

Physical pretreatment of food waste for reducing hydrogen-consuming bacteria

Mahmud

Hee

Ansari

2021

cst

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Food waste is a complex organic substrate that is also potentially hosting a variety of microbial species. In Malaysia, the large amount of food waste that is available can cause health and environmental issues due to inadequate management that has created problems such as greenhouse gas emission and release of wastewater. The utilization of food waste for value-added products is an attractive solution to reduce the accumulation of food waste, however, demands a pretreatment step to prepare the food waste for a specific production process. One interesting application of food waste is for the production of biohydrogen, which requires food waste to be free from hydrogen-consuming bacteria (HCB). This study aims to investigate the effect of physical pretreatment on food waste and suggest the best pretreatment parameters. Food waste samples were pretreated with heat at 70 °C, 80 °C and 90 °C for 15 and 30 minutes and UV radiation for 10, 15 and 30 minutes before being cultured in aerobic condition. The reduction in aerobic bacteria was measured. Heat pretreatment at 70 °C for 15 and 30 minutes could be considered as the best pretreatment compared to the other since it recorded the highest reducing sugar concentration. Heat pretreatment at 70 °C for 15 and 30 minutes and UV radiation pretreatment for 15 minutes had a bactericidal effect and able to remove HCB. There is a high possibility that bacteria 2, 7 and 8 were hydrogen-producing bacteria (HPB).

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“…(Williams andHakam 2016, Hamid et al 2019). The decaying wastes produce weak acidic chemicals, which combine with liquids in the waste to form leachate and landfill gas (Hamid et al 2019). Additionally, inorganic wastes such as electronics and food containers contain toxic elements, particularly heavy metals, including Lead (Pb), Mercury (Hg), Arsenic (As), Cadmium (Cd), and Nickel (Ni) (Popoola et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Characterization and risk evaluation of water samples collected from boreholes situated around a dumpsite in Obalende, Lagos, Nigeria

et al. 2022

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Dumpsites are used worldwide for waste disposal because they are costeffective and have the capacity to contain enormous amounts of waste. However, concerns are rife about the impact of dumpsites on the quality of nearby groundwater. The present study assessed the quality of borehole water near a dumpsite in Obalende, Lagos, Nigeria. Heavy metal, physico-chemical, and microbiological tests were performed on the samples of the water using standard techniques, and the results were compared to the WHO permissible limits. The average daily oral ingestion (ADOI), average daily dermal ingestion (ADDI), and hazard quotient (HQ) of the heavy metals were also estimated. The heavy metal analysis revealed non-permissible levels of zinc, iron, lead, and manganese, while nickel, cadmium, and silicon were within the permissible limits. Physico-chemical analysis showed that turbidity, total suspended solids, total dissolved solids, nitrate, and phosphate were within the permissible limits, but not the pH, electrical conductivity, chloride ion, sulphate and dissolved oxygen. The microbiological examination indicated that the water had high levels of bacteria and coliform counts. The HQ of Zn, Fe, and Pb, mainly through dermal exposure was above the recommended limits (>1). Overall, the results suggest that the water may predispose consumers in the area to Zn, Fe, Pb, and Mn toxicities as well as microbial infections. Consequently, consumers are advised to treat the water before consuming it.

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Isolation and Characterization of Cold Active Bacterial Species from Municipal Solid Waste Landfill Site

Cited by 11 publications

References 15 publications

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Physical pretreatment of food waste for reducing hydrogen-consuming bacteria

Characterization and risk evaluation of water samples collected from boreholes situated around a dumpsite in Obalende, Lagos, Nigeria

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