ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS OF BANGUN-BANGUN (Coleus amboinicus, L.) LEAVES FROM NORTH SUMATERA, INDONESIA

Gurning, Kasta; Haryadi, Winarto; Sastrohamidjojo, Hardjono

doi:10.31788/rjc.2021.1416077

Cited by 11 publications

(11 citation statements)

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“…The selection of the DPPH method in determining antioxidant activity is based on better sensitivity, relatively low cost, and simple and fast processing. The antioxidant activity value of the ethyl acetate fraction of M. calabura leaves expressed in IC 50 from the linear regression equation y = 0.2479x + 36.505; R 2 = 0.9859 The antioxidant activity value of the strong category of ethyl acetate fraction is supported by its ability to release protons to stabilize free radicals from DPPH to DPPH-H so as to produce neutral conditions [2]. These results are in line with the presence of phenolic content which is responsible for providing antioxidant activity.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…In general, the sources of free radicals are divided into reactive oxygen species free radicals and reactive nitrogen species. Low concentrations of free radicals in the body have beneficial roles, but at high concentrations, they can cause oxidative stress and damage cell walls that can trigger the emergence of various chronic and degenerative diseases such as cancer, arthritis, aging, cardiovascular, and neurodegenerative [1], [2]. Minimizing the impact and influence caused by free radicals requires an antioxidant compound.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Total Phenolic Content, Analysis of Bioactive Compound Components, and Antioxidant Activity of Ethyl Acetate Seri (Muntingia calabura L.) Leaves from North Sumatera Province, Indonesia

Situmorang¹,

Gurning²,

Kaban³

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: Seri (Muntingia calabura L.) leaves are a plant that is often found and have not been used in various treatments even though it is reported to have various groups of bioactive compounds such as phenolic, flavonoids, tannins, saponins, steroids, and triterpenoids. AIM: This study aimed to determine the total phenolic content, antioxidant activity and identify the content of potential bioactive compounds contained in the ethyl acetate fraction from M. calabura leaves. METHODS: M. calabura L. leaves fraction was carried out by maceration method using ethanol followed by partition starting with n-hexane, chloroform, and finally ethyl acetate as solvent. The ethyl acetate fraction was continued for phytochemical screening for the content of bioactive compounds using standard reagents, determination of total phenol content by colorimetric method, determination of antioxidant activity using the DPPH method, and analysis of bioactive compounds using gas chromatography–mass spectroscopy. RESULTS: The results showed that the ethyl acetate fraction of M. calabura leaves was positive for phenolic content which was indicated by the formation of a turquoise color after 5% FeCl3 reagent was added (in ethanol), phenolic content was 0.0727 mg GAE/g dry fraction, indicating antioxidant activity (IC50) amounted to 54.437 including strong categories as antioxidants and the results of GC–MS analysis obtained various kinds of compounds and it is suspected that compounds that provide potential as antioxidants are phytol. CONCLUSION: The bioactive compound of ethyl acetate fraction of seri (M. calabura) leaves contained phenolic components and has strong antioxidant activity.

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Section: Antioxidant Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Total Phenolic Content, Analysis of Bioactive Compound Components, and Antioxidant Activity of Ethyl Acetate Seri (Muntingia calabura L.) Leaves from North Sumatera Province, Indonesia

Situmorang¹,

Gurning²,

Kaban³

et al. 2022

Open Access Maced J Med Sci

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“…Identification and analysis of isolates, the obtained isolates were identified by physical form, including the shape and colour of the isolate, melting point, and purity using thin-layer chromatography with n-hexane and ethyl acetate as eluents. Structural analysis was performed using UV-Vis spectrometry, FT-IR (KBr) , 1H& 13 C-NMR spectrometer, and GC-MS so that the molecular structure of the isolate could be determined [15].…”

Section: Methodsmentioning

confidence: 99%

“…The solution mixture was incubated for 30 minutes and then measured using UV-Vis spectrometry at a maximum wavelength of 515 nm. All measurements were performed with three repetitions [15,16].…”

Section: Methodsmentioning

confidence: 99%

“…The analysis showed two absorbance peaks, max 210 nm and 272 nm. The absorbance data isolate at max 210 nm showed the absorbance of methanol (solvent) [15], and the max 272 nm showed the absorbance of active isolates. Furthermore, the absorbance at max 272 nm shows the electronic transition from n→π*, and at wavelength 270-350 shows the electronic transition from n→π* and π→π* [17].…”

Section: Physical Characterization Of An Isolatementioning

confidence: 99%

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Potential antioxidants of secondary metabolite isolates ethyl acetate fraction Coleus amboinicus Lour. Leaves.

Gurning¹,

Haryadi²

2022

SR: PS

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The aim of the study was to isolate and characterize secondary metabolites that have the potential as antioxidants from the ethyl acetate fraction of the leaves of Coleus amboinicus, L. (C. amboinicus). Materials and methods. Purification of the ethyl acetate fraction of C. amboinicus using gravity column chromatography with a stationary phase (silica gel, Merck) and a mobile phase with a solvent ratio of n-hexane (Merck) and ethyl acetate (Merck). Examining isolates includes physical (colour, shape, and melting point). Qualitative purity detection by TLC at 254 nm and 366 nm wavelengths. Structural analysis of metabolites with UV-Vis spectrometer (Spectronic 3000, Genesis 10, Japan), FT-IR(KBr) (Shimadzu IR Prestige-21, Japan), NMR spectrometer (JEOL spectrometer, Japan) operating at 500 MHz (1H-NMR) and 125 MHz (13C-NMR), and Shimadzu's GC-MS (QP-2010S Shimadzu, Japan) and determination of potential antioxidant activity using the DPPH method. Results. The secondary metabolite compounds were isolated in the form of yellow crystals with a melting point of 232-233 °C and Rf values of 0.86 and 0.56, which TLC monitored at a solvent ratio of n-hexane and ethyl acetate 6:4 and 8:2. Spectronic analysis with a UV-Vis Spectrometer showed two electron absorbances, namely a wavelength of 210 nm indicating methanol solvent and 272 nm isolate. The absorbance of functional groups at wave numbers 3379 cm-1 (-OH; hydroxy), 2931 cm-1 (-CH; aliphatic), 1735 cm-1 (-C=O; carbonyl ketone), 1234 cm-1 (-CO-; methoxy) and 1643 cm-1 (-C=C-; alkene). GC-MS analysis obtained two absorbance peaks, (1) the first retention time of 6.658 minutes (3.95 %) and (2) the second retention time of 9.001 minutes (96.05 %). Structural analysis with 1H&13C-NMR showed 28 types of protons and 22 types of carbon. The antioxidant activity potential test showed an activity value (IC50) of 338.54 ppm. Conclusion. The structure of the isolated secondary metabolite compound is 16-acetoxy-7α-hydroxyroyleanone (syn. 16-acetoxyhorminone) and has the potential as an antioxidant.

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Isolation and identification of flavonoida compound from hujan emas leaves (Galphimia speciosa C. E. Anderson)

Sembiring,

Zahara

2023

AIP Conference Proceedings

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ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS OF BANGUN-BANGUN (Coleus amboinicus, L.) LEAVES FROM NORTH SUMATERA, INDONESIA

Cited by 11 publications

References 0 publications

Determination of Total Phenolic Content, Analysis of Bioactive Compound Components, and Antioxidant Activity of Ethyl Acetate Seri (Muntingia calabura L.) Leaves from North Sumatera Province, Indonesia

Determination of Total Phenolic Content, Analysis of Bioactive Compound Components, and Antioxidant Activity of Ethyl Acetate Seri (Muntingia calabura L.) Leaves from North Sumatera Province, Indonesia

Potential antioxidants of secondary metabolite isolates ethyl acetate fraction Coleus amboinicus Lour. Leaves.

Isolation and identification of flavonoida compound from hujan emas leaves (Galphimia speciosa C. E. Anderson)

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