Isolation and Characterization of an AGAMOUS Homolog from Fraxinus pennsylvanica

Du, Ningxia; Pijut, Paula M.

doi:10.1007/s11105-009-0163-7

Cited by 9 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As well as changes in the development of reproductive organs, strong phenotype plants of 35S::PaAG - 1 tobacco also displayed changes in vegetative growth, notably small curled leaves and reduced plant stature. The literature reports that transgenic Arabidopsis plants overexpressing functional AG orthologues show similar vegetative growth defects [12], [13], [35], [36]. Thus, the results of functional analyses in the tobacco host are consistent with the conclusion that PaAG is a floral homeotic C function gene.…”

Section: Discussionsupporting

confidence: 84%

Isolation and Functional Analyses of a Putative Floral Homeotic C-Function Gene in a Basal Eudicot London Plane Tree (Platanus acerifolia)

Zhang

Guo

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

The identification of mutants in model plant species has led to the isolation of the floral homeotic function genes that play crucial roles in flower organ specification. However, floral homeotic C-function genes are rarely studied in basal eudicots. Here, we report the isolation and characterization of the AGAMOUS (AG) orthologous gene (PaAG) from a basal eudicot London plane tree (Platanus acerifolia Willd). Phylogenetic analysis showed that PaAG belongs to the C- clade AG group of genes. PaAG was found to be expressed predominantly in the later developmental stages of male and female inflorescences. Ectopic expression of PaAG-1 in tobacco (Nicotiana tabacum) resulted in morphological alterations of the outer two flower whorls, as well as some defects in vegetative growth. Scanning electron micrographs (SEMs) confirmed homeotic sepal-to-carpel transformation in the transgenic plants. Protein interaction assays in yeast cells indicated that PaAG could interact directly with PaAP3 (a B-class MADS-box protein in P. acerifolia), and also PaSEP1 and PaSEP3 (E-class MADS-box proteins in P. acerifolia). This study performed the functional analysis of AG orthologous genes outside core eudicots and monocots. Our findings demonstrate a conserved functional role of AG homolog in London plane tree, which also represent a contribution towards understanding the molecular mechanisms of flower development in this monoecious tree species.

show abstract

Section: Discussionsupporting

confidence: 84%

Isolation and Functional Analyses of a Putative Floral Homeotic C-Function Gene in a Basal Eudicot London Plane Tree (Platanus acerifolia)

Zhang

Guo

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…a hybridized with sense probe; b PmAG showed expression in the pollen, anthers, and carpel, but not in the corolla. se, sepal; pe, petal; st, stamen; po, pollen (Rutledge et al 1998;Du and Pijut 2010). The exhibited phenotypes of the transgenic plants were normal, except for a change in petal color.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of an AGAMOUS Homologue PmAG from the Japanese Apricot (Prunus mume Sieb. et Zucc.)

et al. 2010

View full text Add to dashboard Cite

An AGAMOUS homologue, named PmAG (GenBank accession number EU068730), was isolated from the Japanese apricot cultivar 'Shirokaga' (Prunus mume) via the homology cloning method. The cDNA was 812-base pairs long with an open reading frame of 732 base pairs, and encoded for a putative protein of 243 amino acid residues. Sequence alignment showed that the sequence of PmAG was 98% identical to that of the peach (Prunus persica). Genomic DNA analysis revealed an intron just before the stop codon of the ORF, and PmAG had AG motifs I and II in its C-terminal region, which suggested that PmAG was an AGAMOUS homologue. Real-time quantitative reverse transcription polymerase chain reaction and in situ hybridization showed that the PmAG mRNA was highly expressed in the sepals, carpel and stamens, and a weak signal was detected in the seed and nutlet. No expression was detected in the leaves or petals. Overexpression of PmAG in transgenic tobacco exhibited no effect on the phenotype of the flower organs, but changes in the floral color from pink to white.

show abstract

“…An alignment of the deduced CsMADS24 protein sequence with that of other AG orthologs from different plant species in literature references was performed by Clustal Omega. The results showed that CsMADS24 was well aligned with the sequences and shared 60.18, 61.54, 63.01, 63.60, 63.98, 64.91, 68.84, 69.01, 70.61, and 71.49% identities with the AG orthologs LLAG1 [11], AtAG [9], OsMADS3 [33], FpAG [12], FaesAG [25], FnAG [18], HpAG [13], MAwuAG [14], PsAG [34], and PrseAG [15], respectively (Fig. 2).…”

Section: Multiple Sequence Alignment and Phylogenetic Analysis Of Csmmentioning

confidence: 87%

“…Overexpression of AG induces a conversion of sepals into carpels and petals into stamens in the flower [9]. To date, the AG orthologs have been identified and characterized from a diverse number of plant species, such as Lilium longiflorum [11], Fraxinus pennsylvanica [12], Hosta plantaginea [13], Magnolia wufengensis [14], Prunus lannesiana [15], Magnolia stellata [16], Carya illinoinensis [17] and Fraxinus nigra [18]. Arabidopsis plants with ectopic expression of AG orthologs from these plants also displayed homeotic changes.…”

Section: Introductionmentioning

confidence: 99%

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Zhou

et al. 2019

ARCH BIOL SCI BELGRA

View full text Add to dashboard Cite

The floral homeotic C-function MADS gene AGAMOUS (AG) in Arabidopsis plays crucial roles in specifying stamen and carpel identities as well as determining floral meristem. However, there have been only a few studies of floral homeotic C-function genes in cucumber thus far. In the present study, CsMADS24, a putative AG ortholog from cucumber, was isolated and characterized. Sequence analysis and protein sequence alignment revealed that the deduced CsMADS24 protein contained the typical MIKC structure and the N-terminal extension, as well as two highly conserved AG motifs (I and II). Phylogenetic analysis showed that CsMADS24 fell into the clade of core eudicots, while being distant from the AG orthologs of basal eudicots, monocots and gymnosperms. Expression analysis by RT-PCR showed that CsMADS24 was exclusively expressed in female flower buds. In situ hybridization revealed that CsMADS24 expression was only detected in the carpels. Functional analyses indicated that the sepals were partly converted into carpelloid-like structures in 35S::35S::CsMADS24 transgenic plants. In addition, earlier flowering and delayed floral organ abscission during the development of siliques were also observed in transgenic Arabidopsis. Our findings demonstrate that the AG ortholog plays an exclusive role in carpel specification of cucumber, providing a basis for revealing the mechanisms of reproductive development in cucumber.

show abstract

Isolation and Characterization of an AGAMOUS Homolog from Fraxinus pennsylvanica

Cited by 9 publications

References 42 publications

Isolation and Functional Analyses of a Putative Floral Homeotic C-Function Gene in a Basal Eudicot London Plane Tree (Platanus acerifolia)

Isolation and Functional Analyses of a Putative Floral Homeotic C-Function Gene in a Basal Eudicot London Plane Tree (Platanus acerifolia)

Isolation and Characterization of an AGAMOUS Homologue PmAG from the Japanese Apricot (Prunus mume Sieb. et Zucc.)

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Contact Info

Product

Resources

About