Isolation and Characterization of an APETALA1-Like Gene from Pear (Pyrus pyrifolia)

Liu, Yuexue; Kong, Jin; Li, Tianzhong; Wang, Yi; Wang, Aide; Han, Zhenhai

doi:10.1007/s11105-012-0540-5

Cited by 12 publications

(7 citation statements)

References 46 publications

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“…The functions of MADS-box genes in development of flower and fruit have also been reported. For example, an AP1-like ( APETALA1-like ) gene was identified in reproductive organ development in Japanese pear ( P. pyrifolia ) ( Liu et al, 2013b ), while ten MADS-box genes were cloned in P. pyrifolia , with their expression during fruit development and ripening analyzed ( Ubi et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of theMADS-boxtranscription factor family in pear (Pyrus bretschneideri) reveals evolution and functional divergence

Wang

Ming

et al. 2017

PeerJ

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MADS-box transcription factors play significant roles in plant developmental processes such as floral organ conformation, flowering time, and fruit development. Pear (Pyrus), as the third-most crucial temperate fruit crop, has been fully sequenced. However, there is limited information about the MADS family and its functional divergence in pear. In this study, a total of 95 MADS-box genes were identified in the pear genome, and classified into two types by phylogenetic analysis. Type I MADS-box genes were divided into three subfamilies and type II genes into 14 subfamilies. Synteny analysis suggested that whole-genome duplications have played key roles in the expansion of the MADS family, followed by rearrangement events. Purifying selection was the primary force driving MADS-box gene evolution in pear, and one gene pairs presented three codon sites under positive selection. Full-scale expression information for PbrMADS genes in vegetative and reproductive organs was provided and proved by transcriptional and reverse transcription PCR analysis. Furthermore, the PbrMADS11(12) gene, together with partners PbMYB10 and PbbHLH3 was confirmed to activate the promoters of the structural genes in anthocyanin pathway of red pear through dual luciferase assay. In addition, the PbrMADS11 and PbrMADS12 were deduced involving in the regulation of anthocyanin synthesis response to light and temperature changes. These results provide a solid foundation for future functional analysis of PbrMADS genes in different biological processes, especially of pigmentation in pear.

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Section: Introductionmentioning

confidence: 99%

Genome-wide identification of theMADS-boxtranscription factor family in pear (Pyrus bretschneideri) reveals evolution and functional divergence

Wang

Ming

et al. 2017

PeerJ

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“…However, functional characterization of AP1 genes in other species is not extensive. Additionally, AP1 homologues are also sometimes expressed in other floral organs like stamen and carpels, but the function of AP1 in these organs is not well defined [ 24 , 37 – 39 ]. The eu FUL genes also have different roles in plant development, being generally involved in the development of leaves, carpels and fruits, and also the maturation process of fleshy fruits [ 40 – 43 ].…”

Section: Introductionmentioning

confidence: 99%

Expression patterns of Passiflora edulis APETALA1/FRUITFULL homologues shed light onto tendril and corona identities

Scorza

Hernandes‐Lopes

Melo-de-Pinna

et al. 2017

EvoDevo

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Background Passiflora (passionflowers) makes an excellent model for studying plant evolutionary development. They are mostly perennial climbers that display axillary tendrils, which are believed to be modifications of the inflorescence. Passionflowers are also recognized by their unique flower features, such as the extra whorls of floral organs composed of corona filaments and membranes enclosing the nectary. Although some work on Passiflora organ ontogeny has been done, the developmental identity of both Passiflora tendrils and the corona is still controversial. Here, we combined ultrastructural analysis and expression patterns of the flower meristem and floral organ identity genes of the MADS-box AP1/FUL clade to reveal a possible role for these genes in the generation of evolutionary novelties in Passiflora.ResultsWe followed the development of structures arising from the axillary meristem from juvenile to adult phase in P. edulis. We further assessed the expression pattern of P. edulis AP1/FUL homologues (PeAP1 and PeFUL), by RT-qPCR and in situ hybridization in several tissues, correlating it with the developmental stages of P. edulis. PeAP1 is expressed only in the reproductive stage, and it is highly expressed in tendrils and in flower meristems from the onset of their development. PeAP1 is also expressed in sepals, petals and in corona filaments, suggesting a novel role for PeAP1 in floral organ diversification. PeFUL presented a broad expression pattern in both vegetative and reproductive tissues, and it is also expressed in fruits.ConclusionsOur results provide new molecular insights into the morphological diversity in the genus Passiflora. Here, we bring new evidence that tendrils are part of the Passiflora inflorescence. This points to the convergence of similar developmental processes involving the recruitment of genes related to flower identity in the origin of tendrils in different plant families. The data obtained also support the hypothesis that the corona filaments are likely sui generis floral organs. Additionally, we provide an indication that PeFUL acts as a coordinator of passionfruit development.Electronic supplementary materialThe online version of this article (doi:10.1186/s13227-017-0066-x) contains supplementary material, which is available to authorized users.

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“…An increase in AP1 expression has been shown to induce early flowering phenotypes in several plant species. Precocious flowering was observed in plants with abnormal overexpression of AP1 including: Arabidopsis, citrus, pear, and roses, among others 38,[55][56][57][58][59][60] .…”

Section: Discussionmentioning

confidence: 99%

The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions

Soares

Weber

Qiu

et al. 2020

Sci Rep

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Shortening the juvenile stage in citrus and inducing early flowering has been the focus of several citrus genetic improvement programs. FLOWERING LOCUS T (FT) is a small phloem-translocated protein that regulates precocious flowering. In this study, two populations of transgenic Carrizo citrange rootstocks expressing either Citrus clementina FT1 or FT3 genes under the control of the Arabidopsis thaliana phloem specific SUCROSE SYNTHASE 2 (AtSUC2) promoter were developed. The transgenic plants were morphologically similar to the non-transgenic controls (non-transgenic Carrizo citrange), however, only AtSUC2-CcFT3 was capable of inducing precocious flowers. The transgenic lines produced flowers 16 months after transformation and flower buds appeared 30–40 days on juvenile immature scions grafted onto transgenic rootstock. Gene expression analysis revealed that the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and APETALA1 (AP1) were enhanced in the transgenics. Transcriptome profiling of a selected transgenic line showed the induction of genes in different groups including: genes from the flowering induction pathway, APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family genes, and jasmonic acid (JA) pathway genes. Altogether, our results suggested that ectopic expression of CcFT3 in phloem tissues of Carrizo citrange triggered the expression of several genes to mediate early flowering.

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Isolation and Characterization of an APETALA1-Like Gene from Pear (Pyrus pyrifolia)

Cited by 12 publications

References 46 publications

Genome-wide identification of theMADS-boxtranscription factor family in pear (Pyrus bretschneideri) reveals evolution and functional divergence

Genome-wide identification of theMADS-boxtranscription factor family in pear (Pyrus bretschneideri) reveals evolution and functional divergence

Expression patterns of Passiflora edulis APETALA1/FRUITFULL homologues shed light onto tendril and corona identities

The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions

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