Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion

Amaro, Itzel; Riaño-Umbarila, Lidia; Becerril, Baltazar; Possani, Lourival D.

doi:10.1016/j.imlet.2011.05.002

Cited by 24 publications

(17 citation statements)

References 41 publications

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“…Two research groups independently isolated scFvs that recognize toxins from the Tityus serrulatus venom, and in both cases, this was carried out after panning human naive libraries [70,71,72]. By opting for this strategy, researchers avoided long-term animal immunization protocols and focused on the expected lack of immunogenicity of human-derived molecules.…”

Section: Preclinical Evaluation Of Recombinant Antibodies For the mentioning

confidence: 99%

“…However, none of the effects were in any case better than those observed with a commercial scorpion antivenom (Butantan Institute) consisting of whole conventional polyclonal IgGs, even if used at a much lower molar concentration as compared to the scFv preparation. Amaro et al reported the selection of another human scFv directed against Ts1 toxin capable of cross-reacting with the venom of other species from the same genus [70]. In vivo assays allowed them to demonstrate the neutralizing capacity of this scFv in mice, but only challenged with 1 LD 50 of Ts1 pre-incubated with a large excess of scFv (molar ratio toxin:scFv: 1:10).…”

Section: Preclinical Evaluation Of Recombinant Antibodies For the mentioning

confidence: 99%

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Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential

Alvarenga

Zahid

Tommaso

et al. 2014

Toxins

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Serum therapy remains the only specific treatment against envenoming, but anti-venoms are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum. Most of these anti-venoms are considered to be efficient, but their production is tedious, and their use may be associated with adverse effects. Recombinant antibodies and smaller functional units are now emerging as credible alternatives and constitute a source of still unexploited biomolecules capable of neutralizing venoms. This review will be a walk through the technologies that have recently been applied leading to novel antibody formats with better properties in terms of homogeneity, specific activity and possible safety.

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Section: Preclinical Evaluation Of Recombinant Antibodies For the mentioning

confidence: 99%

Section: Preclinical Evaluation Of Recombinant Antibodies For the mentioning

confidence: 99%

Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential

Alvarenga

Zahid

Tommaso

et al. 2014

Toxins

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show abstract

“…The serum therapy is indicated in neutralizing venoms inoculated after incidence with venomous animals [10–12]. The serum has some disadvantages, having a series of adverse effects on the victim, such as anaphylaxis and hypersensitivity to heterologous proteins from serum and inefficiency in combating the local effects of the venom [13]. For these reasons, several studies have been conducted in the search for natural compounds that can complement the currently available serotherapy.…”

Section: Introductionmentioning

confidence: 99%

Neutralizing Effects ofMimosa tenuifloraExtracts against Inflammation Caused byTityus serrulatusScorpion Venom

Bitencourt

Lima

Torres-Rêgo

et al. 2014

BioMed Research International

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Scorpion bite represents a significant and serious public health problem in certain regions of Brazil, as well as in other parts of the world. Inflammatory mediators are thought to be involved in the systemic and local immune response induced by Tityus serrulatus scorpion envenomation. The aim of this study was to evaluate the effect of extracts of Mimosa tenuiflora on model envenomation. In mice, the envenomation model is induced by Tityus serrulatus venom. Previous treatment of mice with fractions from M. tenuiflora was able to suppress the cell migration to the peritoneal cavity. The treatment of mice with M. tenuiflora extracts also decreased the levels of IL-6, IL-12, and IL-1β. We concluded that the administration of the extract and fractions resulted in a reduction in cell migration and showed a reduction in the level of proinflammatory cytokines. This study demonstrates, for the first time, the anti-inflammatory effect of aqueous extract from the Mimosa tenuiflora plant on T. serrulatus venom.

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“…Neurotoxins are classified into α-toxins (Ts3 and Ts5) and β-toxins (Ts1 and Ts2). scFv 15e is directed against Ts1, the main toxic component of T. serrulatus venom, and also recognizes toxins from T. cambridgei and T. packyurus , two related scorpions from the same genus [167]. The neutralization capacity of scFv 15e against Ts1 was very limited, as 12 out of 16 mice challenged with one LD 50 of the toxin survived [167].…”

Section: Research Efforts Within Antibodies and Antibody Fragmentsmentioning

confidence: 99%

“…scFv 15e is directed against Ts1, the main toxic component of T. serrulatus venom, and also recognizes toxins from T. cambridgei and T. packyurus , two related scorpions from the same genus [167]. The neutralization capacity of scFv 15e against Ts1 was very limited, as 12 out of 16 mice challenged with one LD 50 of the toxin survived [167]. Currently, optimized variants of scFv 15e are being obtained using a directed evolution approach coupled to phage display with the aim of enhancing its neutralization capacity, but successful results have yet to be reported [167].…”

Section: Research Efforts Within Antibodies and Antibody Fragmentsmentioning

confidence: 99%

Biotechnological Trends in Spider and Scorpion Antivenom Development

Laustsen

Solà

Jappe

et al. 2016

Toxins

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Spiders and scorpions are notorious for their fearful dispositions and their ability to inject venom into prey and predators, causing symptoms such as necrosis, paralysis, and excruciating pain. Information on venom composition and the toxins present in these species is growing due to an interest in using bioactive toxins from spiders and scorpions for drug discovery purposes and for solving crystal structures of membrane-embedded receptors. Additionally, the identification and isolation of a myriad of spider and scorpion toxins has allowed research within next generation antivenoms to progress at an increasingly faster pace. In this review, the current knowledge of spider and scorpion venoms is presented, followed by a discussion of all published biotechnological efforts within development of spider and scorpion antitoxins based on small molecules, antibodies and fragments thereof, and next generation immunization strategies. The increasing number of discovery and development efforts within this field may point towards an upcoming transition from serum-based antivenoms towards therapeutic solutions based on modern biotechnology.

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Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion

Cited by 24 publications

References 41 publications

Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential

Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential

Neutralizing Effects ofMimosa tenuifloraExtracts against Inflammation Caused byTityus serrulatusScorpion Venom

Biotechnological Trends in Spider and Scorpion Antivenom Development

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