Isolation and Characterization of a New Cyanobacterial Strain with a Unique Fatty Acid Composition

Abstract

A new cyanobacterial strain was isolated and purified from salt Lake Balkhash, Kazakhstan. According to its morphological and ultrastructural characteristics, 16S rRNA sequence and the fatty acid profile, the strain has been classified as Cyanobacterium spp. and assigned as Cyanobacterium sp. IPPAS B-1200. The strain is characterized by a non-temperature inducible ∆9-desaturation system, and by high relative amounts of myristic (14:0-30%) and myristoleic (14:1∆9-10%) acids. The total amount of C 14 fatty acids reaches 40%, which is unusually high for cyanobacteria, and it has never been reported before. The remaining fatty acids are represented mainly by palmitic (16:0) and palmitoleic (16:1∆9) acids (the sum reaches nearly 60%). Such a fatty acid composition, together with a relatively high speed of growth, makes this newly isolated strain a prospective candidate for biodiesel production.

Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations

“…Lipid content and fatty acid composition were determined as follows (Sarsekeyeva et al. ). Algae biomass was transferred to 15–50 mL tubes (depending on the volume).…”

“…For determination of lipid content and fatty acid composition samples were withdrawn at the stationary phase of the strain MZ-Ch64 cultures after different nutrients manipulations (i.e., ÀN, ÀP, ÀNÀP). Lipid content and fatty acid composition were determined as follows (Sarsekeyeva et al 2014). Algae biomass was transferred to 15-50 mL tubes (depending on the volume).…”

Draft Genome Sequence of Cyanobacterium sp. Strain IPPAS B-1200 with a Unique Fatty Acid Composition

“…The cyanobacterial strain Cyanobacterium sp. IPPAS B-1200 has been characterized by the unique fatty acid composition of its membrane lipids, which are enriched with myristic (30%), myristoleic (10%), palmitic (40%), and palmitoleic (10%) acids (2). The strain was able to grow intensively at temperatures ranging from 24 to 39°C.…”

Gas Chromatography Mass Spectrometry of <i>Quassia undulata</i> Seed Oil

“…The properties of these triglycerides and their applications depend on the fatty acid constituents (Botinesteanet al, 2012;Moser, 2009). A large variety of fatty acids from algae (Sarsekeyeva et al, 2014;Elumalai and Sakthivel, 2013), animals (Young etal., 2012), edible (Kailet al, 2012) and nonedible (Gashaw and Lakachew, 2014;Ahmad et al, 2011) plants have been investigated for the production of biodiesel.…”