Isolation and characterisation of two multidrug resistance associated protein genes from maize

Swarbreck, David; Ripoll, Pierre-Jean; Brown, Donna A.; Edwards, Keith J.; Theodoulou, F. L.

doi:10.1016/s0378-1119(03)00734-0

Cited by 21 publications

(27 citation statements)

References 25 publications

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“…Within the limits of the differing genetic backgrounds and tissue types examined in these two studies, our results for the expression of ZmMrp1 and ZmMrp2 agree with an RT-PCR based expression analysis reported by Swarbreck et al (2003). The primary difference between the two studies is the detection of very low levels of ZmMrp2 expression in all tissues tested by RT-PCR that were not shown by RNA gel blot analysis.…”

Section: Mrp Expression and Anthocyanin Biosynthesissupporting

confidence: 84%

“…The extensive homology between ZmMrp3 and ZmMrp4 would suggest that these two genes have significant overlap in their substrate specificity, but the smaller size of the transcript indicates a large deletion in the ZmMrp4 gene. The predicted open reading frames for ZmMRP2, AtMRP13, and OsMRP1 are also significantly shorter than the other MRPs and hydropathy plots, and sequence alignments of the predicted proteins indicate that all three MRPs lack the 59 TMD (Sá nchezFerná ndez et al, Jasinski et al, 2003;Swarbreck et al, 2003,). The lack of a 59 TMD domain has also been shown in human Mrp5, a fully active transporter (Bakos et al, 1998).…”

Section: The Lack Of An Aleurone Phenotype Suggests Redundancy In Antmentioning

confidence: 83%

“…This pattern of regulation is consistent with ZmMrp4 playing a role in anthocyanin sequestration in the aleurone and with genetic evidence suggesting that the transport step confers cell autonomy to the anthocyanin pathway. It is interesting to note that the transcript size of ZmMrp4 is significantly shorter than ZmMrp3, possibly reflecting the absence of the 59-most TMD as seen in MRPs identified in maize (Swarbreck et al, 2003) and in many other organisms (Bakos et al, 1998;Kolukisaoglu et al, 2002;Jasinski et al, 2003). Studies to confirm the structure of this Zmrp4 and to test its ability to transport anthocyanin are in progress.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent searches of the publicly available EST database yielded 39 sequence for four additional MRP-like genes. Two of the genes identified in this screen were recently cloned and named ZmMrp1 and ZmMrp2 (Swarbreck et al, 2003). Fragments of the 39 end from each of these 10 genes were used as probes on RNA gel blots to compare expression patterns in eight tissue types using samples from plants with either high (B and Pl ) or no (b and pl ) anthocyanin (Figures 2A and 2B).…”

Section: Mrp Expression and Anthocyanin Biosynthesismentioning

confidence: 99%

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A Multidrug Resistance–Associated Protein Involved in Anthocyanin Transport in Zea mays

2004

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Anthocyanin biosynthesis is one of the most thoroughly studied enzymatic pathways in biology, but little is known about the molecular mechanisms of its final stage: the transport of the anthocyanin pigment into the vacuole. We have identified a multidrug resistance-associated protein (MRP), ZmMrp3, that is required for this transport process in maize (Zea mays). ZmMrp3 expression is controlled by the regulators of anthocyanin biosynthesis and mirrors the expression of other anthocyanin structural genes. Localization of ZmMRP3 in vivo shows its presence in the tonoplast, the site at which anthocyanin transport occurs. Mutants generated using antisense constructs have a distinct pigmentation phenotype in the adult plant that results from a mislocalization of the pigment as well as significant reduction in anthocyanin content, with no alteration in the anthocyanin species produced. Surprisingly, mutant plants did not show a phenotype in the aleurone. This appears to reflect the presence of a second, highly homologous gene, ZmMrp4, that is also coregulated with the anthocyanin pathway but is expressed exclusively in aleurone tissue. This description of a plant MRP with a role in the transport of a known endogenous substrate provides a new model system for examining the biological and biochemical mechanisms involved in the MRP-mediated transport of plant secondary metabolites.

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Section: Mrp Expression and Anthocyanin Biosynthesissupporting

confidence: 84%

Section: The Lack Of An Aleurone Phenotype Suggests Redundancy In Antmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Mrp Expression and Anthocyanin Biosynthesismentioning

confidence: 99%

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A Multidrug Resistance–Associated Protein Involved in Anthocyanin Transport in Zea mays

2004

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“…MRP proteins represent a subfamily of ATP-binding cassette transmembrane transporters widespread in all eukaryotes, which in plants are involved in several functions, such as xenobiotic detoxification, organic ions transport, oxidative stress tolerance (Swarbreck et al, 2003) and even transpiration control (Klein et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait

et al. 2008

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So far, in maize, three classes of mutants involved in phytic acid biosynthesis have been isolated: lpa1, lpa2 and lpa3. In 2007, a gene tagging experiment performed by Shi et al. found that mutations in ZmMRP4 (multidrug resistance-associated proteins 4) gene cause lpa1 phenotype. In previous studies, we isolated and described a single recessive lpa mutation (originally named lpa241), which was allelic to the lpa1-1 mutant, and was consequently renamed lpa1-241. It showed a decrease in the expression of the myo-inositol (Ins)-3-phosphate synthase gene (mips1S). In this study, we present genetic and molecular analyses of the lpa1-241 mutation that indicate an epigenetic origin of this trait, that is, a paramutagenic interaction that results in meiotically heritable changes in ZmMRP4 gene expression, causing a strong pleiotropic effect on the whole plant. The use of a 5-Azacytidine treatment provided data suggesting an association between gene methylation and the lpa1-241 phenotype. To our knowledge, this is the first report of a paramutagenic activity not involving flavonoid biosynthesis in maize, but regarding a key enzyme of an important metabolic pathway in plants.

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Safeners coordinately induce the expression of multiple proteins and MRP transcripts involved in herbicide metabolism and detoxification in Triticum tauschii seedling tissues

Zhang

Lambert

et al. 2007

Proteomics

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Chemicals called safeners protect cereal crops from herbicide toxicity. Proteomic methods (2-D PAGE and LC-MS/MS) were utilized to identify safener- and/or herbicide-regulated proteins in three tissues (root, leaf, and coleoptile) of Triticum tauschii seedlings to better understand a safener's mechanism of action. Growth experiments showed that the safener cloquintocet-mexyl protected seedlings from injury by the herbicide dimethenamid. In total, 29 safener-induced and 10 herbicide-regulated proteins were identified by LC-MS/MS. These proteins were classified into two major categories based on their expression patterns, and were further classified into several functional groups. Surprisingly, mutually exclusive sets of proteins were identified following herbicide or safener treatment, suggesting that different signaling pathways may be recruited. Safener-responsive proteins, mostly involved in xenobiotic detoxification, also included several new proteins that had not been previously identified as safener-responsive, whereas herbicide-regulated proteins belonged to several classes involved in general stress responses. Quantitative RT-PCR revealed that multidrug resistance-associated protein (MRP) transcripts were highly induced by safeners and two MRP genes were differentially expressed. Our results indicate that safeners protect T. tauschii seedlings from herbicide toxicity by coordinately inducing proteins involved in an entire herbicide detoxification pathway mainly in the coleoptile and root, thereby protecting new leaves from herbicide injury.

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Isolation and characterisation of two multidrug resistance associated protein genes from maize

Cited by 21 publications

References 25 publications

A Multidrug Resistance–Associated Protein Involved in Anthocyanin Transport in Zea mays

A Multidrug Resistance–Associated Protein Involved in Anthocyanin Transport in Zea mays

A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait

Safeners coordinately induce the expression of multiple proteins and MRP transcripts involved in herbicide metabolism and detoxification in Triticum tauschii seedling tissues

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