Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells

Malhotra, Rajneesh; Ward, Malcolm; Bright, Helen; Priest, Richard; Foster, Martyn; Hurle, Michael; Blair, E A; Bird, Michael I.

doi:10.1016/s1286-4579(02)00079-5

Cited by 135 publications

(104 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, putative receptor(s) for RSV was described to have L-selectin-like properties [39]. L-Selectin is a member of the adhesion-molecule selectin family and is important in leukocyte tethering for endothelial transmigration during normal trafficking or at sites of inflammation [40].…”

Section: Discussionmentioning

confidence: 99%

“…L-Selectin is a member of the adhesion-molecule selectin family and is important in leukocyte tethering for endothelial transmigration during normal trafficking or at sites of inflammation [40]. One of the proposed RSV receptors was further characterized as annexin II [39]. Annexin II belongs to the annexin family of calcium-and phospholipid-binding proteins.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Reduced clearance of respiratory syncytial virus infection in a preterm lamb model

Meyerholz

Grubor

Fach

et al. 2004

Microbes and Infection

View full text Add to dashboard Cite

Respiratory syncytial virus (RSV) causes significant respiratory disease in children worldwide. For the study of severe RSV disease seen in preterm infants, a suitable animal model is lacking. The novel hypothesis of this study was that preterm lambs are susceptible to bovine RSV (bRSV) infection, an analogous pneumovirus with ruminant host specificity, and that there would be age-dependent differences in select RSV disease parameters. During RSV infection, preterm lambs had elevated temperatures and respiration rates with mild anorexia and cough compared to controls. Gross lesions included multifocal consolidation and atelectasis with foci of hyperinflation. Microscopic lesions included multifocal alveolar septal thickening and bronchiolitis. Immunohistochemistry localized the RSV antigen to all layers of bronchiolar epithelium from a few basal cells to numerous sloughing epithelia. A few mononuclear cells were also immunoreactive. To assess for age-dependent differences in RSV infection, neonatal lambs were infected similarly to the preterm lambs or with a high-titer viral inoculum. Using morphometry at day 7 of infection, preterm lambs had significantly more cellular immunoreactivity for RSV antigen (P <0.05) and syncytial cell formation (P <0.05) than either group of neonatal lambs. This work suggests that perinatal RSV clearance is age-dependent, which may explain the severity of RSV infection in preterm infants. The preterm lamb model is useful for assessing agedependent mechanisms of severe RSV infection. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. AbstractRespiratory syncytial virus (RSV) causes significant respiratory disease in children worldwide. For the study of severe RSV disease seen in preterm infants, a suitable animal model is lacking. The novel hypothesis of this study was that preterm lambs are susceptible to bovine RSV (bRSV) infection, an analogous pneumovirus with ruminant host specificity, and that there would be age-dependent differences in select RSV disease parameters. During RSV infection, preterm lambs had elevated temperatures and respiration rates with mild anorexia and cough compared to controls. Gross lesions included multifocal consolidation and atelectasis with foci of hyperinflation. Microscopic lesions included multifocal alveolar septal thickening and bronchiolitis. Immunohistochemistry localized the RSV antigen to all layers of bronchiolar epithelium from a few basal cells to numerous sloughing epithelia. A few mononuclear cells were also immunoreactive. To assess for age-dependent differences in RSV infection, neonatal lambs were infected similarly to the preterm lambs or with a high-titer viral inoculum. Using morphometry at day 7 of infection, preterm lambs had significantly more cellular immunoreactivity for RSV antigen (P <0.05) and syncytial cell formation (P <0.05) than either group of neonatal lambs. This work suggests that peri...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Reduced clearance of respiratory syncytial virus infection in a preterm lamb model

Meyerholz

Grubor

Fach

et al. 2004

Microbes and Infection

View full text Add to dashboard Cite

show abstract

“…Furthermore, the conserved cysteine rich region has homology with the CX3C chemokine motif, which may facilitate infection by interacting with the CX3C chemokine receptor, CX3CR1 [146]. The G protein may also interact with L-selectin (CD62L), annexin II [90], and surfactant proteins [48,62]. The role of the G protein in the pathogenesis of BRSV infection will be discussed below.…”

Section: Glycoprotein Gmentioning

confidence: 99%

Bovine respiratory syncytial virus infection

Valarcher¹,

2007

View full text Add to dashboard Cite

-Bovine respiratory syncytial virus (BRSV) belongs to the pneumovirus genus within the family Paramyxoviridae and is a major cause of respiratory disease in young calves. BRSV is enveloped and contains a negative sense, single-stranded RNA genome encoding 11 proteins. The virus replicates predominantly in ciliated respiratory epithelial cells but also in type II pneumocytes. It appears to cause little or no cytopathology in ciliated epithelial cell cultures in vitro, suggesting that much of the pathology is due to the host's response to virus infection. RSV infection induces an array of pro-inflammatory chemokines and cytokines that recruit neutrophils, macrophages and lymphocytes to the respiratory tract resulting in respiratory disease. Although the mechanisms responsible for induction of these chemokines and cytokines are unclear, studies on the closely related human (H)RSV suggest that activation of NF-κB via TLR4 and TLR3 signalling pathways is involved. An understanding of the mechanisms by which BRSV is able to establish infection and induce an inflammatory response has been facilitated by advances in reverse genetics, which have enabled manipulation of the virus genome. These studies have demonstrated an important role for the non-structural proteins in anti-interferon activity, a role for a virokinin, released during proteolytic cleavage of the fusion protein, in the inflammatory response and a role for the SH and the secreted form of the G protein in establishing pulmonary infection. Knowledge gained from these studies has also provided the opportunity to develop safe, stable, live attenuated virus vaccine candidates.

show abstract

“…Rabbit vesivirus (RaV) virions might also depend on interaction with AnxA2 for their attachment and internalization (González-Reyes et al, 2009). Moreover, AnxA2 was isolated as a potential respiratory syncytial virus (RSV) receptor on epithelial cells (Malhotra et al, 2003). AnxA2 was reported as a receptor for Pseudomonas aeruginosa (Kirschnek et al, 2005), but has been recently implicated in Mycoplasma infection as well.…”

Section: Annexins As Host Cell Surface Receptors For Microbesmentioning

confidence: 99%

Emerging functions as host cell factors – an encyclopedia of annexin-pathogen interactions

Kuehnl

Musiol

Raabe

et al. 2016

Biological Chemistry

View full text Add to dashboard Cite

Abstract:Emerging infectious diseases and drug-resistant infectious agents call for the development of innovative antimicrobial strategies. With pathogenicity now considered to arise from the complex and bi-directional interplay between a microbe and the host, host cell factor targeting has emerged as a promising approach that might overcome the limitations of classical antimicrobial drug development and could open up novel and efficient therapeutic strategies. Interaction with and modulation of host cell membranes is a recurrent theme in the host-microbe relationship. In this review, we provide an overview of what is currently known about the role of the Ca 2+ dependent, membrane-binding annexin protein family in pathogenhost interactions, and discuss their emerging functions as host cell derived auxiliary proteins in microbe-host interactions and host cell targets.

show abstract

Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells

Cited by 135 publications

References 33 publications

Reduced clearance of respiratory syncytial virus infection in a preterm lamb model

Reduced clearance of respiratory syncytial virus infection in a preterm lamb model

Bovine respiratory syncytial virus infection

Emerging functions as host cell factors – an encyclopedia of annexin-pathogen interactions

Contact Info

Product

Resources

About