Isolasi Dan Seleksi Bakteri Penghasil Biosurfaktan Yang Terdapat Di Dalam Deposit Lilin Pada Pipa Transmisi Minyak Mentah

Amilia, Amilia; Tyas, Mardya Ning; Juliani, Any; Yulianto, Andik

doi:10.20885/khazanah.vol5.iss2.art5

Cited by 2 publications

(2 citation statements)

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“…Adapun tujuan dilakukan identifikasi molekuler pada bakteri adalah untuk memperoleh rangkaian DNA serta untuk mengetahui spesies dari bakteri tersebut. Penelitian Amilia et al, (2013) ditemukannya Enterobacter gergoviae yang di isolasi dari deposit lilin pada pipa transisi minyak mentah yang terbukti memiliki kemampuan dalam memecah hidrokarbon pada minyak mentah sehingga bakteri tersebut dapat mendegradasi minyak pada perairan yang telah tercemar dengan menghasilkan senyawa biosurfaktan.…”

Section: Identifikasi Molekuler Bakteri (Pcr/ Polymerase Chain Reaction)unclassified

Isolation and Identification of Biosurfactant-Producing Bacteria in An-aerobic Palm Oil Waste Pools at PT. Aek Loba Plantation

Sipahutar,

Rasyidah,

Mayasari

2024

JBT

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Biosurfactant-producing bacteria are bacteria that have alternative energy sources, namely surfactants which contain hydrophilic and hydrophobic groups based on their molecular structure so that they can survive between different polar and hydrogen bonding fluids. Palm oil mill liquid waste is waste in the form of water or liquid, oil and organic solids produced from the processing of palm oil. The purpose of this study was to determine the genera of biosurfactant-producing bacteria found in palm oil wastewater in the an-aerobic pond of PT. Aek Loba plantation and to find out the bacterial species with the highest emulsification index. This study used descriptive methods and identification of bacteria through morphological characterization, gram staining tests and biochemical tests. Bacteria were isolated from palm oil wastewater by dilution method 10-4, 10-5 and 10-6. Bacterial emulsification index is calculated by emulsification test. In this study, 8 biosurfactant-producing bacterial isolates were found, consisting of 2 Proteus genus isolates, 2 Enterobacter genus isolates, 2 Bacillus genus bacterial isolates and 2 Pluralibacter genus isolates. The bacteria that had the highest emulsification index was Pluralibacter gergoviae with an emulsion index of 43% which was identified by molecular testing.

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Section: Identifikasi Molekuler Bakteri (Pcr/ Polymerase Chain Reaction)unclassified

Isolation and Identification of Biosurfactant-Producing Bacteria in An-aerobic Palm Oil Waste Pools at PT. Aek Loba Plantation

Sipahutar,

Rasyidah,

Mayasari

2024

JBT

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“…In the electrocoagulation process at the anode an oxidation reaction occurs to the anion (negative ion), an anode made of metal such as iron will undergo an oxidation reaction to form Fe (OH)2 [27], while at the cathode hydrogen gas will form which functions to lift the flock Fe (OH)2 onto the surface. Flocks that form over time will increase in size and eventually settle to the bottom of the electrocoagulation bioreactor.…”

Section: The Effect Of Electrocoagulation Techniques On Tss Concentra...mentioning

confidence: 99%

POME Processing with Bioremediation Using Indigenous Bacteria (Bacillus toyonensis and Stenotrophomonas rhizophila), Electrocoagulation, and Bioremediation assisted with Electrocoagulation Methods

Yudono¹,

Ulfariani²,

Fatma³

et al. 2022

IJFAC

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Palm oil mill effluent (POME) is wastewater containing dissolved materials with concentrations above the threshold. Generally, POME processing in Indonesia uses an open pond system. It is less efficient because it requires large areas and causing greenhouse gas effects. This research aims to improve the POME processing, three methods were studied; bioremediation using enrich indigent lypholitics bacteria (Bacillus toyonensis and Stenotrophomonas rhizophila.), electrocoagulation system, and combination of those methods. The parameters were measured were oil and fat contents, COD, pH, TSS, TDS, Fe and Cu metals. In the electrocoagulation method, the electrodes were used iron electrodes with a variation of electrical voltage 1.5, 3, 4.5 and 6 V. The bioremediation using indigent bacteria results in the removal of oil and fat -74.62%, COD -52.44%, TSS -43.11% and pH 6.38 at the incubation time for 6 days, TDS 21.42%, Fe -5.3% and Cu -64.70%. The electrocoagulation methods results oil and fat removal were -93.27%, COD was -85.71%, TDS -39%, TSS -84%, Cu -46%, Fe +92.35%, and pH 11.39 at a voltage of 6 V for 6 h. The combination of the two methods can reduce oil and fat levels by -87.39%, COD -56.70%, TDS -0.42%, TSS +15.88%, Cu -23.33%, Fe +93.74% and pH 8.82. The three methods were studied can improve the POME processing significantly.

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Isolasi Dan Seleksi Bakteri Penghasil Biosurfaktan Yang Terdapat Di Dalam Deposit Lilin Pada Pipa Transmisi Minyak Mentah

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Cited by 2 publications

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Isolation and Identification of Biosurfactant-Producing Bacteria in An-aerobic Palm Oil Waste Pools at PT. Aek Loba Plantation

Isolation and Identification of Biosurfactant-Producing Bacteria in An-aerobic Palm Oil Waste Pools at PT. Aek Loba Plantation

POME Processing with Bioremediation Using Indigenous Bacteria (Bacillus toyonensis and Stenotrophomonas rhizophila), Electrocoagulation, and Bioremediation assisted with Electrocoagulation Methods

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