Isogenic lines in fish – a critical review

Franěk, Roman; Baloch, Abdul Rasheed; Kašpar, Vojtěch; Saito, Taiju; Fujimoto, Takafumi; Arai, Katsutoshi; Pšenička, Martin

doi:10.1111/raq.12389

Cited by 37 publications

(37 citation statements)

References 226 publications

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“…More advanced application of surrogacy such as production of donor-derived gametes from cryopreserved germ cells, or production of monosex female stocks based on oogonia transplantation carrying XX chromosomes will be attempted further. Of the utmost importance, results of this study give us a positive outlook for oncoming reproduction of gold sh chimeras which received cells from a homozygous individual when production of genetically identical gametes and creation of an isogenic line is foreseen [54]. On the other hand, potential of highly fertile common carp recipients will be advantageous to rapidly increase production of small gold sh.…”

Section: Discussionmentioning

confidence: 90%

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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Background: Common carp is the fourth most-produced species in worldwide aquaculture. Significant efforts are invested in breeding and preservation of genetic integrity of this important species. However, maintaining carp gene bank in situ can be considered as demanding due to its big body size. Recent progress in reproductive biotechnologies in fish allows improving some unfavourable characteristics of a target species using surrogate reproduction. Germ stem cells (gamete precursors) from one species are transplanted into different surrogate species with small body size. After maturation, surrogates are producing donor-derived progeny. Efficient protocols for cryopreservation of carp male and female germ stem cells have been developed lately. Thus, the next logical goal was to assess the potential of goldfish surrogate to produce donor-derived gametes of common carp after intraperitoneal transplantation of testicular cells. Results: High transplantation success was achieved when 44% of the surviving goldfish produced pure donor-derived gametes of common carp. More importantly, both viable eggs and sperm giving rise to pure common carp progeny were produced, witnessing sustainability of the presented method. Donor-derived identity of the offspring was confirmed by genotyping and typical phenotype corresponding to the donor species. Reproductive performance of chimeras was similar to goldfish controls. Assessment of gamete characteristics showed that the size of donor-derived eggs is between control carp and goldfish eggs. Interestingly, flagellum length in donor-derived spermatozoa was comparable to common carp flagellum and significantly shorter than goldfish flagellum. Conclusions: In this study, we succeeded in the production of pure common carp progeny from surrogate goldfish recipients transplanted intraperitoneally by testicular germ cells. Here we reported production of viable eggs between most distant species up to date. Good reproductive performance of goldfish germline chimeras gives a promising prospect for further analysis about the long-term reproductive performance of surrogates, recovery of cryopreserved germ cells or production of monosex stocks. Presented technology is ready to ease needs for carp breeds preservation and their recovery using many times smaller goldfish surrogates.

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Section: Discussionmentioning

confidence: 90%

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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“…Doubled haploid isogenic lines have been established in several fish species (Franěk et al, 2019). They offer unparalleled opportunities for a number of in-depth studies of complex traits.…”

Section: Isogenic Doubled Haploid Lines a Tool Of Choice To Unravel mentioning

confidence: 99%

Combining Multiple Approaches and Models to Dissect the Genetic Architecture of Resistance to Infections in Fish

et al. 2020

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“…Poor reproductive performance of doubled haploid progenitors has been highlighted as a major limitation in the production of isogenic lines [9,60]. The relative fecundity (eggs/kg female bodyweight) of doubled haploid rainbow trout was normal and even significantly higher than controls [7], but with observations of sterile and hypofertile fish, and homozygous carp females had severe gonadal defects and less than 10% could be reproduced [5].…”

Section: Production Of Clonal Linesmentioning

confidence: 99%

Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines

Hansen

Penman

Glover

et al. 2020

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Background In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research tools in genome studies as well as in studies of commercially important traits. The Atlantic salmon is one of the best studied fish species globally, but all experiments are done on fish of wild or domesticated origin and access to standardized immortal fish lines would be of great benefit. Here, we describe the protocols developed to produce mitotic gynogenes, and from these the first clonal lines in Atlantic salmon. Results Atlantic salmon eggs fertilized with UV irradiated sperm combined with a pressure shock applied at 4700–4800 minC at 8 oC gave all homozygous (doubled haploid) gynogenetic progeny with high survival. From the six first maturing females, five all homozygous clonal lines were produced by meiotic gynogenesis and were verified as clonal and identical to their mother with microsatellite markers. Conclusions We have now produced the first documented cloned Atlantic salmon lines. This work demonstrates the potential for production of further Atlantic salmon clonal lines, potentially with distinct characteristics. Such lines will provide an important resource for further elucidation of phenotypic and genetic traits in this globally important species.

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Isogenic lines in fish – a critical review

Cited by 37 publications

References 226 publications

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Combining Multiple Approaches and Models to Dissect the Genetic Architecture of Resistance to Infections in Fish

Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines

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