Isoflavonoid accumulation in soybean roots infected with vesicular-arbuscular mycorrhizal fungi

Morandi, Dominique; Bailey, J. A.; Gianinazzi‐Pearson, Vivienne

doi:10.1016/0048-4059(84)90009-2

Cited by 171 publications

(76 citation statements)

References 16 publications

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“…Flavonoids such as coumestrol and daidzein have been shown to stimulate AMF colonization in a variety of plants (see review by Vierheilig et al, 1998), and their synthesis appears to increase in the presence of the fungi even before root colonization (Larose et al, 2002). Even though various changes in the pattern of flavonoid accumulation have been reported (e.g., Morandi et al, 1984;Harrison and Dixon, 1993;Larose et al, 2002), the information in the literature is inconsistent. Bécard et al (1995) suggested that flavonoids are not essential as stimulatory compounds for AMF.…”

Section: Introductionmentioning

confidence: 75%

Specific flavonoids as interconnecting signals in the tripartite symbiosis formed by arbuscular mycorrhizal fungi, Bradyrhizobium japonicum (Kirchner) Jordan and soybean (Glycine max (L.) Merr.)

Antunes

Rajcan

Goss

2006

Soil Biology and Biochemistry

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Many legume plants benefit from the tripartite symbiosis of arbuscular mycorrhizal fungi (AMF) and rhizobia. Beneficial effects for the plant have been assumed to rely on increased P supply through the mycorrhizas. Recently, we demonstrated that P does not regulate the establishment of the tripartite symbiosis. Flavonoids appear to play a role as early signals for both rhizobia and AMF. Four soybean lines known to express different concentrations of the isoflavones genistein, daidzein and glycitein in the seed were used to test three hypotheses: (i) The establishment of the tripartite symbiosis is not dependent of a nutrient mediated effect; (ii) There is a positive correlation between seed isoflavone concentrations of different soybean lines and the progress of the tripartite symbiosis; (iii) Specific flavonoids control the establishment of the tripartite symbiosis in that a change in flavonoid root accumulation resulting from the development of one microbial partner can stimulate colonization of soybean roots by the other. Disturbed versus undisturbed soil treatments were produced to vary the potential for indigenous AMF colonization of soybean. In contrast, the potential for Bradyrhizobium was kept identical in both soil disturbance treatments. The uptake of P and Zn and the concentration of flavonoids in mycorrhizal soybean roots at 10 d after emergence were analysed either separately of Bradyrhizobium or in context of the tripartite symbiosis. Zinc nutrition did not differ between AMF treatments which supports the first hypothesis. The concentration of daidzein was at least four times greater in the root than in the seed reaching 3958G249 mg g K1 dry across soybean lines. Coumestrol, which was absent in the seed, was synthesized to reach 2154G64 mg g K1 dry. Conversely, the concentration of genistein was approximately three times smaller in the root that in the seed (301G15 mg g K1 dry), while glycitein and formononetin were never detected. The establishment of the tripartite symbiosis was identical across soybean lines which does not support the second hypothesis. Concentrations of flavonoids were significantly greater in roots under disturbed soil, for which both symbioses were not as developed as in plants from undisturbed soil. This clearly supports the third hypothesis. This research provides the first data linking the function of different flavonoids to the establishment of the tripartite symbiosis, and suggests that these compounds are produced and released into the rhizosphere as a function of the colonization process. q

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Section: Introductionmentioning

confidence: 75%

Specific flavonoids as interconnecting signals in the tripartite symbiosis formed by arbuscular mycorrhizal fungi, Bradyrhizobium japonicum (Kirchner) Jordan and soybean (Glycine max (L.) Merr.)

Antunes

Rajcan

Goss

2006

Soil Biology and Biochemistry

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“…The concentration of flavonoids in roots of leguminous plants also varies with the root colonization by AMF (Volpin et al, 1994;Morandi & Bailey, 1984). Alfalfa plants inoculated with Glomus intraradices have higher concentrations of formononetin, and the variations observed resulted from the presence of the AMF (plant defense response) and were not due to the effect of the fungus in the nutritional status of the plant, since the inoculated plants and the uninoculated plants had the same growth (Volpin et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, variations observed in the concentration of the compound represented in peak 1 could be a result of the reduced plant growth due to nutritional stress (phosphorus deficiency). Besides the nutritional stress, variations in the synthesis of secondary metabolites may result from plant defense response induced by presence of the mycorrhizal fungi, as demonstrated in roots of leguminous plants inoculated with AMF (Volpin et al, 1994;Morandi & Bailey, 1984), even though it seems that mycorrhizal fungi do not elicit typical plant defense response, or such response is rapidly suppressed by yet unknown mechanisms (Gadkar et al, 2001). …”

Section: Resultsmentioning

confidence: 99%

“…Alfalfa plants inoculated with Glomus intraradices have higher concentrations of formononetin, and the variations observed resulted from the presence of the AMF (plant defense response) and were not due to the effect of the fungus in the nutritional status of the plant, since the inoculated plants and the uninoculated plants had the same growth (Volpin et al, 1994). Analyzing the contents of stress metabolites in soybean roots inoculated with Glomus mosseae or Glomus fasciculatum or a mixed inoculum of the two species, Morandi & Bailey (1984) observed a greater accumulation of gliceolin, cumestrol, and daidzein in the colonized roots, when compared to the uncolonized roots and that soybean roots colonized with G. mosseae, and G. fasciculatum had higher levels of coumestrol than the roots colonized by only G. mosseae. Morandi & Bailey (1984) observed also an increase in the contents of daidzein in soybean roots after four weeks of colonization with G. mosseae or G. fasciculatum, and after six weeks of colonization with a mixed inoculum of the two species; after eight weeks, the high levels of this compound were maintained only in the roots colonized with G. fasciculatum.…”

Section: Resultsmentioning

confidence: 99%

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Arbuscular mycorrhizal fungi and the occurrence of flavonoids in roots of passion fruit seedlings

Soares¹,

Martins²,

Mathias³

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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Composition and the role of root flavonoids in the regulation of mycorrhizal symbiosis are still poorly understood. Several flavonoids stimulate spore germination, mycelia growth and root colonization by arbuscular mycorrhizal fungi (AMF), and both root colonization and flavonoid composition are affected by plant nutritional status. Effects of AMF on the occurrence and content of aromatic secondary metabolites in the roots of passion fruit seedlings grown under two levels of phosphorus (P) fertilization (10 and 50 mg kg -1 of phosphorus) was studied. Seedlings were inoculated with Glomus clarum and a population of native fungi from a passion fruit plantation. Methanolic extracts of passion fruit seedlings roots were analyzed by high performance liquid chromatography (HPLC). It was recorded the occurrence of several compounds, possibly flavonoids, with seven major peaks. The root contents of the compound with a retention time of 4.5 minutes, varied in response to the root colonization by different mycorrhizal fungi, and the contents of two compounds with retention times of 3.4 and 18.9 minutes varied due to the poor plant growth and nutritional status. Passion fruit seedlings have several aromatic compounds, and their contents were correlated with root colonization by different mycorrhizal fungi, the reduced seedling growth due to nutritional stress, and/or the plant defense responses to the fungi. Key words: phenolic compounds, mycorrhizal plants, phosphorus nutrition FUNGOS MICORRÍZICOS ARBUSCULARES E A OCORRÊNCIA DE FLAVONÓIDES EM RAÍZES DE MUDAS DE MARACUJAZEIRO AMARELORESUMO: Os flavonóides nas raízes e seu papel na regulação da simbiose com fungos micorrízicos não são bem conhecidos. Vários flavonóides estimulam a germinação de esporos, crescimento micelial e colonização micorrízica. Ambos, a colonização micorrízica e a composição de flavonóides nas raízes são afetados pelo estado nutricional da planta. Avaliou-se o efeito de fungos micorrízicos arbusculares sobre a ocorrência e concentração de substâncias, possivelmente metabólitos aromáticos secundários pertencentes à classe dos flavonóides, em raízes de mudas de maracujazeiro amarelo, crescidas sob dois níveis de fósforo (10 e 50 mg kg -1). As mudas foram inoculadas com Glomus clarum e uma população nativa de fungos micorrízicos de um plantio de maracujá do município de São João da Barra, RJ. Extratos de raízes em metanol foram analisados por cromatografia líquida de alta eficiência. Os cromatogramas mostraram a ocorrência de diversos compostos, possivelmente da classe dos flavonóides, diferenciados no cromatograma em sete picos principais. Foi observado que os conteúdos nas raízes do composto com tempo de retenção de 4,5 minutos, variou em função da colonização micorrízica pelas diferentes espécies de fungos e o conteúdo dos dois compostos com tempo de retenção de 3,4 e 18,9 minutos, variou com o baixo crescimento da planta e o estresse nutricional. Mudas de maracujazeiro apresentam diversos compostos aromáticos, possivelmente da classe dos fl...

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“…Production of toxic substances by the VAM fungi themselves has not yet been demonstrated but there is evidence that mycorrhizal roots contain increased concentrations of isoflavonoids (Morandi, Bailey & Gianinazzi-Pearson 1984;Morandi & GianinazziPearson 1986). Accumulation of these metabolites in the roots are considered to be involved in the mechanism by which the formation of mycorrhizal roots has increased plant resistance to damage by plant-parasitic nematodes.…”

mentioning

confidence: 99%

Interactions between mycophagous nematodes, mycorrhizal and other soil fungi

Giannakis¹,

Sanders²

1990

Agriculture, Ecosystems & Environment

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Isoflavonoid accumulation in soybean roots infected with vesicular-arbuscular mycorrhizal fungi

Cited by 171 publications

References 16 publications

Specific flavonoids as interconnecting signals in the tripartite symbiosis formed by arbuscular mycorrhizal fungi, Bradyrhizobium japonicum (Kirchner) Jordan and soybean (Glycine max (L.) Merr.)

Specific flavonoids as interconnecting signals in the tripartite symbiosis formed by arbuscular mycorrhizal fungi, Bradyrhizobium japonicum (Kirchner) Jordan and soybean (Glycine max (L.) Merr.)

Arbuscular mycorrhizal fungi and the occurrence of flavonoids in roots of passion fruit seedlings

Interactions between mycophagous nematodes, mycorrhizal and other soil fungi

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