Isoenzymatic variability among five Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera of the Amazon region, Brazil

Abstract

An isoenzymatic comparative analysis of the variability and genetics differentiation among

Limited Diversity of Anopheles Darlingi in the Peruvian Amazon Region of Iquitos

“…21 In Brazil, a within-population diversity for An. darlingi of 0.171 was found, 20 and in the same species in seven countries of Central and South America a range of 0.063 to 0.122 was found. 13 The level of differentiation among populations in the studied area was very low ( ␤ , an F ST estimator, is 0.0125).…”

“…10 Genetic diversity of Anopheles has been studied using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), [11][12][13][14][15] internal transcribed spacer 2 (ITS2) markers, 13,16 microsatellite loci, 17 mitochondrial DNA, 17,18 single nucleotide polymorphism genotyping, 19 and isoenzymatic analysis. 13,20 RAPD-PCR is based on the amplification of random DNA using short sequence primers (approximately 10 bases) at low annealing temperatures. It has been used to analyze the genetic variation of An.…”

The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy: a critical review

“…Cytogenetics has proven immensely useful for differentiating among sympatric anopheline taxa, however, with a few exceptions (Pérez & Conn 1992, Ramirez & Dessen 2000, this technique has not been applied for neotropical anophelines. Ecological (Guimarães et al 2004), behavioral (da Silva Vasconcelos et al 2002 and biochemical (Phillips et al 1988, dos Santos et al 2003) studies also provide important information for mosquito taxonomy and should be encouraged.…”

Isoenzymatic analysis of four Anopheles (Kerteszia) bellator Dyar & Knab (Diptera: Culicidae) populations

“…Interest in better morphological characterization and in intrapopulational comparisons of Neotropical anopheline species has increased during the last few years, mostly focusing on characterization of cryptic species complexes (Conn 1993, Linley et al 1996, Lounibos et al 1998, Rosa-Freitas et al 1998, Manguin et al 1999, Calle et al 2002, Sallum et al 2002, Santos et al 2003. Most of these studies concern subgenus Nyssorhynchus species due to their major role in malaria transmission in South and Central America.…”