Isodon rugosus (Wall. ex Benth.) Codd In Vitro Cultures: Establishment, Phytochemical Characterization and In Vitro Antioxidant and Anti-Aging Activities

Abbasi, Bilal Haider; Siddiquah, Aisha; Tungmunnithum, Duangjai; Bose, Shankhamala; Younas, Muhammad; Garros, Laurine; Drouet, Samantha; Giglioli-Guivarc’h, Nathalie; Hano, Christophe

doi:10.3390/ijms20020452

Cited by 40 publications

(47 citation statements)

References 66 publications

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“…No report is present on the production of phenolic compounds in the adventitious root culture of L. usitatissimum until now. However, recently many researchers reported an improvement in the production of phenolics and flavonoids in elicited-cell cultures of L. usitatissimum [13][14][15][16]49], which also endorsed our results. Moreover, many researchers also reported that adventitious root cultures were more potent than callus cultures in the production of phenolic compounds [50].…”

Section: Phenolics Production In Callus and Adventitious Root Culturesupporting

confidence: 90%

“…Adventitious roots showed greater antioxidant activity than callus culture, probably due to higher production of phenolics than callus culture. Studies on the antioxidant properties of in vitro cultures of L. usitatissimum are scarce, and only a few reports are available on the antioxidant activity of its callus, hairy root cultures and cell suspension [13][14][15][16]31,32,49]. These investigators reported higher antioxidant activity in hairy root and cell suspension cultures than the callus culture of flax [31].…”

Section: Antioxidant Activity Of Callus and Adventitious Root Culturesmentioning

confidence: 99%

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Feasible Production of Lignans and Neolignans in Root-Derived In Vitro Cultures of Flax (Linum usitatissimum L.)

Anjum

Komal

Drouet

et al. 2020

Plants

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Flax lignans and neolignans impart health benefits, particularly in treating different types of cancers, due to their strong phytoestrogenic and antioxidant properties. The present study enhances the comprehension on the biosynthesis of antioxidant lignans and neolignans in root-derived in vitro cultures of flax (both callus and adventitious root). The results presented here clearly showed that the adventitious root culture efficiently produced a higher amount of lignans (at day 40) and neolignans (at day 30) than callus culture of flax. High performance liquid chromatography (HPLC) analysis revealed that the accumulations of secoisolariciresinol diglucoside (SDG, 5.5 mg g−1 DW (dry weight)) and dehydrodiconiferyl alcohol glucoside (DCG, 21.6 mg/g DW) were 2-fold higher, while guaiacylglycerol-β-coniferyl alcohol ether glucoside (GGCG, 4.9 mg/g DW) and lariciresinol glucoside (LDG, 11.9 mg/g DW) contents were 1.5-fold higher in adventitious root culture than in callus culture. Furthermore, the highest level of total phenolic production (119.01 mg/L), with an antioxidant free radical scavenging activity of 91.01%, was found in adventitious root culture at day 40, while the maximum level of total flavonoid production (45.51 mg/L) was observed in callus culture at day 30 of growth dynamics. These results suggest that adventitious root culture can be a good candidate for scaling up to industrial level to commercially produce these pharmacologically and nutritionally valuable metabolites.

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Section: Phenolics Production In Callus and Adventitious Root Culturesupporting

confidence: 90%

Section: Antioxidant Activity Of Callus and Adventitious Root Culturesmentioning

confidence: 99%

Feasible Production of Lignans and Neolignans in Root-Derived In Vitro Cultures of Flax (Linum usitatissimum L.)

Anjum

Komal

Drouet

et al. 2020

Plants

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“…Methylation was higher in callus compared with leaves, and the most methylated sequences were CG context and CXG context, whereas CXX sequences essentially lack methylation (Zakrzewski et al 2017). Recent bisulfite sequencing of some maize genome regions (Abbasi et al 2019) revealed that de novo methylation of CG sequence prevailed over methylation of CXG sequence, and the CXX sequence had the lowest methylation levels. Our results showed that de novo methylation was higher than demethylation in the CXX sequence, which is consistent with the results for the control (M10) trial but not for the M13 trial that verified maximum variation.…”

Section: Discussionmentioning

confidence: 99%

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

Orłowska

Bednarek

2020

Plant Mol Biol

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Key message The Taguchi method and metAFLP analysis were used to optimise barley regenerants towards maximum andminimum levels of tissue culture-induced variation. The subtle effects of symmetric and asymmetric methylationchanges in regenerants were identified. Abstract Plant tissue cultures (PTCs) provide researchers with unique materials that accelerate the development of new breeding cultivars and facilitate studies on off-type regenerants. The emerging variability of regenerants derived from PTCs may have both genetic and epigenetic origins, and may be desirable or degrade the value of regenerated plants. Thus, it is crucial to determine how the PTC variation level can be controlled. The easiest way to manipulate total tissue cultureinduced variation (TTCIV) is to utilise appropriate stress factors and suitable medium components. This study describes the optimisation of in vitro tissue culture-induced variation in plant regenerants derived from barley anther culture, and maximizes and minimizes regenerant variation compared with the source explants. The approach relied on methylation amplified fragment length polymorphism (metAFLP)-derived TTCIV characteristics, which were evaluated in regenerants derived under distinct tissue culture conditions and analysed via Taguchi statistics. The factors that may trigger TTCIV included CuSO 4 , AgNO 3 and the total time spent on the induction medium. The donor plants prepared for regeneration purposes had 5.75% and 2.01% polymorphic metAFLP loci with methylation and sequence changes, respectively. The level of TTCIV (as the sum of all metAFLP characteristics analyzed) identified in optimisation and verification experiments reached 7.51 and 10.46%, respectively. In the trial designed to produce a minimum number of differences between donor and regenerant plants, CuSO 4 and AgNO 3 were more crucial than time, which was not a significant factor. In the trial designed to produce a maximum number of differences between donor and regenerant plants, all factors had comparable impact on variation. The Taguchi method reduced the time required for experimental trials compared with a grid method and suggested that medium modifications were required to control regenerant variation. Finally, the effects of symmetric and asymmetric methylation changes on regenerants were identified using novel aspects of the metAFLP method developed for this analysis.

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“…After extraction, each extract was centrifuged for 15 min at 3,000 rpm and the resulting supernatant filtered using a syringe filter (0.45 µ m, Millipore, Molsheim, France) prior to analysis. The total phenolic content (TPC) was determined spectrophotometrically using the Folin-Ciocalteu reagent (Sigma Aldrich, Saint-Quentin Fallavier, France) and according to the protocol adapted for microplate reader described by Abbasi et al [17]. Briefly, 10 µ L of extract were homogenized with 180 µ L of a mixture composed of 4% Na2CO3 (prepared in NaOH 0.1 M), 0.02% potassium sodium tartrate tetrahydrate and 0.02% CuSO4.…”

Section: Ultrasound-assisted Extraction Methods Developmentmentioning

confidence: 99%

A Quick, Green and Simple Ultrasound-Assisted Extraction for the Valorization of Antioxidant Phenolic Acids from Moroccan Almond (<em>Prunus dulcis</em> (Mill.) D.A.Webb) Cold-Pressed Oil Residues

Elamrani¹,

Abid²,

Drouet³

et al. 2020

Preprint

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Almond (Prunus dulcis (Mill.) D.A.Webb) is one of the most important nut crops both in terms of area and production. Over the last decades, an important part of the beneficial actions for health associated with their consumption was attributed to the phenolic compounds, mainly accumulated in almond skin. Interestingly, after cold-pressed oil extraction, most of these antioxidant phenolic compounds are retained in a skin-enriched by-product, so-called almond cold-pressed oil residue. In Morocco, ranked fifth producer in the world, this production generates an important part of this valuable byproduct. In the present study, using a multivariate Box-Behnken design, an ultrasound-assisted extraction (USAE) method of phenolic compounds from Moroccan almond cold-pressed oil residue was developed and validated. Response surface methodology resulted in the optimal extraction conditions: the use of aqueous EtOH 53.0% (v/v) as green solvent, applying an US frequency of 27.0 kHz for an extraction duration of 29.4 min. The present USAE allowed substantial gains in terms of extraction efficiency compared to conventional heat reflux extraction. Applied to 3 different local Beldi genotypes growing at 3 different experimental sites, the optimal USAE conditions led to a total phenolic content of 13.86 mg/g dry weight (DW). HPLC analysis revealed that the main phenolic compounds from this valuable byproduct were: chlorogenic acid followed by protocatechuic acid, p-hydrobenzoic acid and p-coumaric acid. The accumulation of these phenolic compounds appeared to be more dependent on the genetic background than on the environmental impact here represented by the 3 experimental culture sites. Both in vitro cell free and cellular antioxidant assays were performed, and revealed the great potential of these extracts. In particular, correlation analysis evidenced the prominent roles of chlorogenic acid, protocatechuic acid and p-hydrobenzoic acid. To summarize, the USAE method presented here is a quick, green, simple and efficient validated USAE for the possible valorization of antioxidant phenolic compounds from Moroccan almond cold-Pressed oil residues, making it possible to generate extracts with attractive antioxidant activities for future nutraceutical and/or cosmetic applications.

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Isodon rugosus (Wall. ex Benth.) Codd In Vitro Cultures: Establishment, Phytochemical Characterization and In Vitro Antioxidant and Anti-Aging Activities

Cited by 40 publications

References 66 publications

Feasible Production of Lignans and Neolignans in Root-Derived In Vitro Cultures of Flax (Linum usitatissimum L.)

Feasible Production of Lignans and Neolignans in Root-Derived In Vitro Cultures of Flax (Linum usitatissimum L.)

Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley

A Quick, Green and Simple Ultrasound-Assisted Extraction for the Valorization of Antioxidant Phenolic Acids from Moroccan Almond (<em>Prunus dulcis</em> (Mill.) D.A.Webb) Cold-Pressed Oil Residues

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