ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980 The GenBank accession number for the sequence reported in this paper is AF162268.

Egelseer, Eva M.; Idris, Rughia; Jarosch, Marina; Danhorn, Thomas; Sleytr, Uwe B.; Sára, Margit

doi:10.1099/00221287-146-9-2175

Cited by 16 publications

(16 citation statements)

References 40 publications

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“…PCR analysis revealed that the coding region of the sbsC gene was interrupted by a new type of IS element designated ISBst12, which currently cannot be attributed to any of the known IS families. Southern hybridization showed that ISBst12 was present in multiple copies in the S-layer-carrying strain, as well as in that with the S-layer-deficient phenotype (34). Interestingly, ISBst12 was also detected in B. stearothermophilus PV72/p6, its oxygen-induced strain variant PV72/p2, and the strain with the S-layer-deficient phenotype (PV72/T5).…”

Section: S-layer-deficient Phenotypes and S-layer Variationmentioning

confidence: 85%

S-Layer Proteins

2000

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Section: S-layer-deficient Phenotypes and S-layer Variationmentioning

confidence: 85%

S-Layer Proteins

2000

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“…In G. stearothermophilus strain variants, expression of a completely new type of S‐layer protein is accompanied by synthesis of a different type of SCWP and S‐layer variation may also lead to a change in the lattice type (Sára et al , 1994, 1996). Regarding the development of S‐layer‐deficient strain variants, the importance of insertion sequence (IS) elements has been demonstrated for various organisms (Egelseer et al , 2000; Scholz et al , 2000).…”

Section: Introductionmentioning

confidence: 99%

S-layers as a tool kit for nanobiotechnological applications

Sleytr

Huber

Ilk

et al. 2007

FEMS Microbiology Letters

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Crystalline bacterial cell surface layers (S-layers) have been identified in a great number of different species of bacteria and represent an almost universal feature of archaea. Isolated native S-layer proteins and S-layer fusion proteins incorporating functional sequences self-assemble into monomolecular crystalline arrays in suspension, on a great variety of solid substrates and on various lipid structures including planar membranes and liposomes. S-layers have proven to be particularly suited as building blocks and patterning elements in a biomolecular construction kit involving all major classes of biological molecules (proteins, lipids, glycans, nucleic acids and combinations of them) enabling innovative approaches for the controlled 'bottom-up' assembly of functional supramolecular structures and devices. Here, we review the basic principles of S-layer proteins and the application potential of S-layers in nanobiotechnology and biomimetics including life and nonlife sciences.

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“…A second group of closely related IS, widely spread among both bacteria and Archaea are thought to represent a subgroup within the IS66 family (194). The founder member, ISBst12, originally isolated from Bacillus stearothermophilus, was described as a novel family (195), but identification of many additional examples suggests that the ISBst12 and IS66 groups should be considered a single family. ISBst12 are found in Actinobacteria, Cyanobacteria, Deinococcus/Thermus, Firmicutes, and Planctomycetes as well as in Proteobacteria.…”

Section: Is66 (I) Generalmentioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

et al. 2015

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The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

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ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980 The GenBank accession number for the sequence reported in this paper is AF162268.

Cited by 16 publications

References 40 publications

S-Layer Proteins

S-Layer Proteins

S-layers as a tool kit for nanobiotechnological applications

Everyman's Guide to Bacterial Insertion Sequences

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