Is α-chloralose plus halothane induction a suitable anesthetic regimen for cerebrovascular research?

Bonvento, Gilles; Charbonne, R.; Corrèze, Jean-Loup; Borredon, Josiane; Seylaz, Jacques; Lacombe, Pierre

doi:10.1016/0006-8993(94)91340-4

Cited by 85 publications

(47 citation statements)

References 24 publications

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“…Rats were anesthetized with α-chloralose to minimize anesthesiainduced depression of neuronal activity (21)(22)(23), after which a cranial window was created over the somatosensory cortex for in vivo assessment using an optical/fluorescence imaging (OFI) platform (SI Appendix, Fig. S1).…”

Section: Resultsmentioning

confidence: 99%

“…To minimize this confound and make it relevant to fMRI studies we chose α-chloralose, which has been widely used for rodent fMRI studies because (i) it preserves metabolic coupling for somatosensory stimulation (21), (ii) it provides a normal CBF baseline close to that measured in the awake state compared with other anesthetic agents such as isoflurane (22), and (iii) it preserves cerebrovascular reactivity (23). In addition, because of the slow Ca 2+ i acquisition rate used, we cannot rule out the possibility that the "slow" Ca 2+ i oscillations might be due to folding over from higher- (49).…”

Section: Discussionmentioning

confidence: 99%

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Low-frequency calcium oscillations accompany deoxyhemoglobin oscillations in rat somatosensory cortex

Volkow

Koretsky

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Spontaneous low-frequency oscillations (LFOs) of blood-oxygenlevel-dependent (BOLD) signals are used to map brain functional connectivity with functional MRI, but their source is not well understood. Here we used optical imaging to assess whether LFOs from vascular signals covary with oscillatory intracellular calcium (Ca 2+ i ) and with local field potentials in the rat's somatosensory cortex. We observed that the frequency of Ca 2+ i oscillations in tissue (∼0.07 Hz) was similar to the LFOs of deoxyhemoglobin (HbR) and oxyhemoglobin (HbO 2 ) in both large blood vessels and capillaries. The HbR and HbO 2 fluctuations within tissue correlated with Ca 2+ i oscillations with a lag time of ∼5-6 s. The Ca 2+ i and hemoglobin oscillations were insensitive to hypercapnia. In contrast, cerebral-blood-flow velocity (CBFv) in arteries and veins fluctuated at a higher frequency (∼0.12 Hz) and was sensitive to hypercapnia. However, in parenchymal tissue, CBFv oscillated with peaks at both ∼0.06 Hz and ∼0.12 Hz. Although the higher-frequency CBFv oscillation (∼0.12 Hz) was decreased by hypercapnia, its lower-frequency component (∼0.06 Hz) was not. The sensitivity of the higher CBF V oscillations to hypercapnia, which triggers blood vessel vasodilation, suggests its dependence on vascular effects that are distinct from the LFOs detected in HbR, HbO 2 , Ca 2+ i , and the lower-frequency tissue CBFv, which were insensitive to hypercapnia. Hemodynamic LFOs correlated both with Ca 2+ i and neuronal firing (local field potentials), indicating that they directly reflect neuronal activity (perhaps also glial). These findings show that HbR fluctuations (basis of BOLD oscillations) are linked to oscillatory cellular activity and detectable throughout the vascular tree (arteries, capillaries, and veins).spontaneous low-frequency brain oscillations | resting-state functional connectivity | neuronal calcium | cerebral hemodynamic | neuroimaging M easures of resting-state functional connectivity with functional MRI (fMRI) are based on spontaneous low-frequency blood-oxygen-level-dependent (BOLD) oscillations that occur throughout the brain with the assumption that regions with correlated oscillations are functionally connected (1, 2). The networks that emerge from resting-state functional connectivity correspond roughly with neuroanatomical connectivity (3, 4) and are modified by brain diseases (5-7). BOLD signals in fMRI reflect the interplay between hemodynamics (including blood volume and velocity of blood flowing in the vessels) and cellular (neuronal and glial) metabolism, which affect the amount of deoxygenated hemoglobin (HbR) in brain tissue that leads to changes in BOLD fMRI (8,9). Human studies using near-infrared spectroscopy (NIRS) (10) have reported low-frequency oscillations (LFOs) of ∼0.04-0.1 Hz for oxygenated hemoglobin (HbO 2 ) and HbR in the brain consistent with those measured by BOLD (11). However, there is still no quantitative understanding of the relative direct contribution of spontaneous oscillations in cellular...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Low-frequency calcium oscillations accompany deoxyhemoglobin oscillations in rat somatosensory cortex

Volkow

Koretsky

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…This anesthetic allows studying the physiology of functional brain activation (Leniger-Follert and Hossmann, 1979;Bonvento et al, 1994) and preserves the cerebrovascular coupling (Ueki et al, 1992;Lindauer et al, 1993). In the ventilation gas mixture, N 2 O was replaced by N 2 (Gyngell et al, 1996).…”

Section: Methodsmentioning

confidence: 99%

Differential Effects of NMDA and AMPA Glutamate Receptors on Functional Magnetic Resonance Imaging Signals and Evoked Neuronal Activity during Forepaw Stimulation of the Rat

et al. 2006

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Most of the currently used methods for functional brain imaging do not visualize neuronal activity directly but rather rely on the elicited 1-(4-aminophenyl)-3-methylcarbamyl-4-methyl7,8-methylenedioxy-3,4-dihydro-5H-2,3benzodiazepine] significantly decreased both the hemodynamic response (BOLD, Ϫ49 Ϯ 13 and Ϫ65 Ϯ 15%; PWI, Ϫ22 Ϯ 48 and Ϫ68 Ϯ 4% for 5 and 7 mg/kg, i.v., respectively; CBV, Ϫ80 Ϯ 7% for 7 mg/kg; n ϭ 4) and the SEPs (up to Ϫ60%). These data indicate that the interaction of glutamate with its postsynaptic and/or glial receptors is necessary for the generation of blood flow and BOLD responses and illustrate the differential role of NMDA-Rs and AMPA-Rs in the signaling chain leading from increased neuronal activity to the hemodynamic response in the somatosensory cortex.

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“…We used laser-Doppler flowmetry to study cerebral blood flow (CBF) in mice (Bonvento et al 1994). Surgical preparation was carried out on anesthetized mice with inhaled halothane.…”

Section: Cerebral Blood Flow Reactivitymentioning

confidence: 99%

Notch3 is required for arterial identity and maturation of vascular smooth muscle cells

Domenga¹,

Fardoux²,

Lacombe³

et al. 2004

Genes Dev.

Self Cite

472

463

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Formation of a fully functional artery proceeds through a multistep process. Here we show that Notch3 is required to generate functional arteries in mice by regulating arterial differentiation and maturation of vascular smooth muscle cells (vSMC). In adult Notch3−/− mice distal arteries exhibit structural defects and arterial myogenic responses are defective. The postnatal maturation stage of vSMC is deficient in Notch3 −/− mice. We further show that Notch3 is required for arterial specification of vSMC but not of endothelial cells. Our data reveal Notch3 to be the first cell-autonomous regulator of arterial differentiation and maturation of vSMC.Supplemental material is available at http://www.genesdev.org.

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Is α-chloralose plus halothane induction a suitable anesthetic regimen for cerebrovascular research?

Cited by 85 publications

References 24 publications

Low-frequency calcium oscillations accompany deoxyhemoglobin oscillations in rat somatosensory cortex

Low-frequency calcium oscillations accompany deoxyhemoglobin oscillations in rat somatosensory cortex

Differential Effects of NMDA and AMPA Glutamate Receptors on Functional Magnetic Resonance Imaging Signals and Evoked Neuronal Activity during Forepaw Stimulation of the Rat

Notch3 is required for arterial identity and maturation of vascular smooth muscle cells

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