Is Root Catalase a Bifunctional Catalase-Peroxidase?

Chioti, Vasileia; Zervoudakis, George

doi:10.3390/antiox6020039

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…(22) We found a report in the PubMed database that was allegedly associated with low catalase activity and reduced catalase protection against free radicals in the body. (5,23) However, the data of the present study did not show a significant association between the CAT gene and the clinical condition of the HbE/thalassemia β patients. In the case of minor thalassemia, there is also a change in oxidative reactions, which are thought to be associated with hypochromic microcytic anemia, similar to iron deficiency anemia.…”

Section: Resultscontrasting

confidence: 93%

Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia

et al. 2020

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Background Thalassemia, a monogenic genetic disease of red blood cells, is spread widely throughout the world. Glutathione S transferase (GST) enzymes have an antioxidant role in detoxification processes of toxic substances This study aimed to determine the role of the genetic modifier genes GSTT1 and GSTM1, and the catalase (CAT) gene in clinical degrees of hemoglobin (Hb)E/β thalassemia. Methods Sixty HbE/β Thalassemia patients were examined to determine their clinical pictures. Clinical score was based on age when thalassemia symptoms appeared, time of diagnosis, time of first blood transfusion, pre-transfusion hemoglobin concentration, frequency of transfusions, and enlargement of spleen. Ferritin concentration was also obtained from medical records. Gene polymorphisms of GSTT1, GSTM1, and CAT were measured using PCR and PCR-RFLP methods. Clinical scores were categorized into mild (0-3.5), moderate (4-7), and severe (7.5-10) degrees, while ferritin level was expressed in mg/dL. One way Anova was used to analyze the data. Results The clinical appearance showed that severe, moderate, and mild degrees accounted for 42%, 45%, and 13%, respectively. The majority had a high ferritin level of more than 5000 mg/dL (67%). GSTT1 null, GSTM1 null, and CAT minor allele genotypes were 21.7%, 33.3%, and 12.1%, respectively. GSTT1, GSTM1, and CAT genotypes had no impact on the severity of thalassemia patients (p=0.091, p=0.082, and p=0.141, respectively). Conclusion GSTT1, GSTM1, CAT gene polymorphisms tend to be a minor aspect of severity of clinical outcome for HbE/â thalassemia patients and should be not considered a routine laboratory check.

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Section: Resultscontrasting

confidence: 93%

Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia

et al. 2020

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“…In tobacco, another isoform of CAT, which has enhanced peroxidatic activity (47), was reported to increase in seedlings grown at high CO 2 (46). Decreased sensitivity to 3-aminotriazole, an inhibitor of the classical CAT reaction, suggests that the root isoforms may have greater bifunctional peroxidase activities (20).…”

Section: Prmentioning

confidence: 99%

Redox Homeostasis and Signaling in a Higher-CO₂World

Foyer

Noctor

2020

Annu. Rev. Plant Biol.

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Rising CO2 concentrations and their effects on plant productivity present challenging issues. Effects on the photosynthesis/photorespiration balance and changes in primary metabolism are known, caused by the competitive interaction of CO2 and O2 at the active site of ribulose-1,5-bisphosphate carboxylase/oxygenase. However, impacts on stress resistance are less clear. Reactive oxygen species are key players in biotic and abiotic stress responses, but there is no consensus on whether elevated CO2 constitutes a stress. Although high CO2 increases yield in C3 plants, it can also increase cellular oxidation and activate phytohormone defense pathways. Reduction-oxidation processes play key roles in acclimation to high CO2, with specific enzymes acting in compartment-specific signaling. Traditionally, acclimation to high CO2 has been considered in terms of altered carbon gain, but emerging evidence suggests that CO2 is a signal as well as a substrate. Some CO2 effects on defense are likely mediated independently of primary metabolism. Nonetheless, primary photosynthetic metabolism is highly integrated with defense and stress signaling pathways, meaning that plants will be able to acclimate to the changing environment over the coming decades.

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“…Salt tolerant plants maintain physiological levels of H 2 O 2 by deploying multiple enzymatic (SOD, CAT, GPOD, APOD, and GR) and non-enzymatic (ascorbate and glutathione) components of an antioxidant defense system [ 60 ]. CAT is a monofunctional enzyme and converts two molecules of H 2 O 2 into one molecule of water and molecular oxygen each [ 73 ]. CAT removes the bulk of the H 2 O 2 from the system, but has a low affinity for H 2 O 2 due to its high K m value for H 2 O 2 [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Salinity Induced Antioxidant Defense in Roots of Industrial Hemp (IH: Cannabis sativa L.) for Fiber during Seed Germination

Dixit

2022

Antioxidants

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Global climate change induced sea level rise, rainfed agriculture, poor quality irrigation water, and seawater intrusion through interconnected ditches and inland waterways cause soil salinity in inland and coastal areas. To reclaim and prevent further soil erosion, salt tolerant crops are required. Industrial Hemp (IH: Cannabis sativa L.) is used for food, fiber, and medicinal purposes throughout the world. In spite of that, little is known about the salt tolerance mechanisms in IH. Seed germination and development of the roots are the primary events in the life cycle of a plant, which directly interact with soil salinity. Therefore, in vitro germination experiments were conducted on the roots of 5-day-old seedlings using four varieties (V1: CFX-2, V2: Joey, V3: Bialobrzeskie, and V4: Henola) of IH for fiber. Five salinity treatments (0, 50, 80, 100, 150, and 200 mM NaCl) were used to screen the IH varieties on the basis of I: seed germination percent (SGP), II: quantitative morphological observations (root length (RL) and root fresh weight (RFW)), III: oxidative stress indices (hydrogen peroxide (H2O2) and lipid peroxidation), and IV: antioxidant defense system comprises of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPOD), ascorbate peroxidase (APOD), glutathione reductase (GR). The varieties V1 and V3 showed salt tolerance up to 100 mM by maintaining higher SGP, less reduction in RL and RFW. These roots in V1 and V3 showed lower levels of H2O2 and lipid peroxidation by displaying higher activities of SOD, CAT, GPOD, APOD, and GR while a reciprocal trend was observed in V4. However, roots in V2 showed higher activities of antioxidant enzymes with lower levels of H2O2 and lipid peroxidation, but showed declines in RL and RFW at 80 mM NaCl onward. Roots in V4 were the most susceptible to NaCl stress at 50 mM and onward.

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Is Root Catalase a Bifunctional Catalase-Peroxidase?

Cited by 11 publications

References 23 publications

Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia

Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia

Redox Homeostasis and Signaling in a Higher-CO₂World

Salinity Induced Antioxidant Defense in Roots of Industrial Hemp (IH: Cannabis sativa L.) for Fiber during Seed Germination

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Is Root Catalase a Bifunctional Catalase-Peroxidase?

Cited by 11 publications

References 23 publications

Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia

Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia

Redox Homeostasis and Signaling in a Higher-CO2World

Salinity Induced Antioxidant Defense in Roots of Industrial Hemp (IH: Cannabis sativa L.) for Fiber during Seed Germination

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Redox Homeostasis and Signaling in a Higher-CO₂World