Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.01.056 View full text
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Zhenqing Li, Xiaoming Dou, Yi Ni, Qinmiao Chen, Shuyi Cheng, Yoshinori Yamaguchi

Abstract: Pulsed field capillary electrophoresis (PFCE) is a predominant technique to cope with difficulties in resolving large DNA strands, yet it is still unclear whether pulsed electric field is effective for the separation of higher mass RNA. In this paper we focused on the role of pulsed electric field in large RNA fragments analysis by comparing RNA separation performance in PFCE with that in constant field CE. Separation performance in terms of migration mobility, plate numbers, resolution, and selectivity has be…

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