Is oxytocin an ovarian hormone?

Wathes, D C; Swann, R. W.

doi:10.1038/297225a0

Cited by 273 publications

(116 citation statements)

References 22 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…In this respect, horses clearly differ from domestic ruminants, in which there is substantial evidence that the corpus luteum synthesizes and secretes oxytocin during the cycle (Wathes & Swann, 1982;Ivell & Richter, 1984;Swann et ai, 1984) and that this peptide is involved in the regulation of luteolysis (Flint & Sheldrick, 1983). Our results also differ from previous reports of oxytocin concentrations in mares' plasma (Burns et ai, 1981;Tetzke et ai, 1987).…”

Section: Discussioncontrasting

confidence: 56%

Measurement of oxytocin concentrations in plasma and ovarian extracts during the oestrous cycle of mares

Stevenson¹,

Parkinson²,

Wathes³

1991

Reproduction

View full text Add to dashboard Cite

Summary. Jugular venous blood samples were collected throughout a complete oestrous cycle from 9 mares for measurement of progesterone and oxytocin by radioimmunoassay. Mean oxytocin concentrations remained at \m=~\ 1 pg/ml throughout, with no evidence of cyclic variation in the release pattern. Extracts of corpus luteum and follicles obtained from a further 33 mares at different stages of the cycle all contained oxytocin concentrations of < 10 pg/g wet weight of tissue. We conclude that the ovaries are not a source of circulating oxytocin during the oestrous cycle in this species. The plasma oxytocin concentrations reported here are substantially lower than those found by other groups.

show abstract

Section: Discussioncontrasting

confidence: 56%

Measurement of oxytocin concentrations in plasma and ovarian extracts during the oestrous cycle of mares

Stevenson¹,

Parkinson²,

Wathes³

1991

Reproduction

View full text Add to dashboard Cite

show abstract

“…Unlike the short half-life of exogenous supplements of oxytocin, the plasma elevations seen in the present mice are a consistent and reproducible effect [30], making bacteria or bacterial products a possible therapy for mental health. It remains to be proven whether microbe-induced oxytocin in these murine models originates primarily from the hypothalamus or from other peripheral sources [72][73][74][75][76]. Nonetheless, our results suggest that gut bacteriainduced oxytocin may explain data linking gut microbiome dysbiosis with neuropsychological disorders, including autism [77,78].…”

Section: Discussionmentioning

confidence: 92%

Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of Obesity

Varian¹,

Levkovich²,

Poutahidis³

et al. 2017

J Prob Health

View full text Add to dashboard Cite

AnimalsC57BL/6 wild type (wt), oxytocin-wt (oxt-wt) and oxytocin- AbstractCohabitation with pet dogs imparts diverse health benefits to humans including a slim physique. It is known that neuropeptide hormone oxytocin fundamental in human-canine social bonds regulates appetite and body weight. It was recently shown in mice that consuming Lactobacillus reuteri ATCC 6475 from human breast milk lowers body weight and up-regulates oxytocin levels in blood. Here we test the hypothesis that bacteria from dog saliva may similarly modulate recipient host body weight. We find that a Lactobacillus spp isolate from dog saliva led to lower body weight when fed to C57BL/6 wild type mice. Mice consuming the canineborne L. reuteri also had elevated oxytocin levels in blood plasma, and exhibited body weight in an oxytocin-dependent manner. Interestingly, killed (lysed) canine bacteria were sufficient to achieve the physiological effects. Taken together, these studies provide evidence that dog bacteria modulate oxytocin levels and body weight in recipient mice, and thus may help reduce risk of obesity in individuals cohabitating with pet dogs. knockout (oxt-ko) B6; 129S-Oxttm1Wsy/J mice (purchased initially from Jackson labs; Bar Harbor, ME) were used in three separate experiments (Figure 1). Mice were housed and handled in Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited facilities using techniques and diets including Lactobacillus reuteri as specifically approved by Massachusetts Institute of Technology's (MIT) Committee on Animal Care (CAC). Mice were housed under standard 12:12 light cycle conditions with lights on at 7 AM. Mice were fed a standard control chow Purina RMH3000. Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of ObesityMice were bred in-house to achieve experimental groups. Each experiment included 5-15 animals per group with one or two replications (total N=10-30 mice examined per group) unless otherwise specified. For the initial studies, C57BL/6 wt mice received Lactobacillus reuteri 2546 isolated from dog saliva. To test putative roles for microbeinduced oxytocin in obesity, oxt-ko mice and their oxt-wt littermates entered experiments at eight weeks of age. At the conclusion of the study mice were euthanized with CO 2 overdose, and were examined as described below. Eight pet dogs served as saliva microbe donors as approved by the MIT-CAC. Saliva was collected from these dogs in the morning before feeding using sterile swabs [Puritan Sterile Polyester Tipped applicators Guilford, Me Ref: 25-806 1PD] in 1.5 ml centrifuge tubes (Safeseal Microcentrifuge Tubes, Sorenson Bioscience Inc. Salt Lace City, UT Cat# 16070). Bacteria were purified from dog saliva as described in detail below. PCR of dog saliva for all Lactobacillus speciesSaliva was collected from eight pet dogs and prepared using High Pure PCR Template kit (Roche Diagnostics) was used without changes to the manufacturer's directions to isolate DNA from the canine saliva. DNA was measured using Nan...

show abstract

“…Oxytocin is a major product of the corpus luteum in ruminants (Wathes & Swann, 1982), but its role during the oestrous cycle is far from certain. Whilst there is good evidence for an involvement in luteolysis (see Auletta &Flint, 1988 andWathes, 1989 for reviews), mean circulating oxytocin concentrations are highest in the early to midluteal phase (Webb et ai, 1981 ;Schams et ai, 1982) and receptor concentrations in the reproductive tract peak on the day of oestrus Ayad & Wathes, 1989;Ayad et ai, 1990a).…”

Section: Introductionmentioning

confidence: 99%

Influence of oxytocin infusion during oestrus and the early luteal phase on progesterone secretion and the establishment of pregnancy in ewes

Wathes

Ayad²,

Gilbert³

et al. 1991

Reproduction

View full text Add to dashboard Cite

Summary. In Experiment 1, an osmotic minipump containing oxytocin was implanted s.c. in ewes for 12 days beginning on Day 10 of the oestrous cycle, producing \m=~\ 100 pg oxytocin/ml in the plasma. Two days after the start of infusion, all ewes were injected with 100 \g=m\gcloprostenol and placed with a fertile ram. At slaughter 22 days later, 9 (75%) of the 12 control (saline-infused) ewes were pregnant compared with 1 (11%) of the 9 ewes infused with oxytocin. In the control group, midcycle plasma concentrations of oxytocin were significantly higher in nonpregnant than in pregnant ewes.In Experiment 2, an infertile ram was used throughout to avoid any possible effects of pregnancy and oxytocin infusions were given at different stages of the oestrous cycle. Otherwise the protocol was similar to that in Exp. 1. Oxytocin infusion during luteolysis and the early follicular phase had no effect on the subsequent progesterone secretion pattern, but infusions beginning the day before cloprostenol-induced luteolysis and lasting for 7 or 12 days and infusions beginning on the day of oestrus for 4 days all delayed the subsequent rise in plasma progesterone by \ m=~\3\p=n-\4 days. In these animals, the cycle tended to be longer.It was concluded that an appropriate oxytocin secretion pattern may be necessary for the establishment of pregnancy in ewes and that a high circulating oxytocin concentration during the early luteal phase delays the development of the young corpus luteum.

show abstract

Is oxytocin an ovarian hormone?

Cited by 273 publications

References 22 publications

Measurement of oxytocin concentrations in plasma and ovarian extracts during the oestrous cycle of mares

Measurement of oxytocin concentrations in plasma and ovarian extracts during the oestrous cycle of mares

Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of Obesity

Influence of oxytocin infusion during oestrus and the early luteal phase on progesterone secretion and the establishment of pregnancy in ewes

Contact Info

Product

Resources

About