Iron oxide labelling of human mesenchymal stem cells in collagen hydrogels for articular cartilage repair

Heymer, A; Haddad, Daniel; Weber, M.; Gbureck, Uwe; Jakob, Peter M.; Eulert, J.; Nöth, Ulrich

doi:10.1016/j.biomaterials.2007.12.003

Cited by 112 publications

(110 citation statements)

References 45 publications

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“…Moreover, iron oxide labeling of chondrocytes may have immediate applicability in current tissue repair therapies such as autologous chondrocyte implantation. [39][40][41][42][43] Heymer et al 44 were the first to report on SPIO labeling specifically for the purposes of cartilage tissue engineering. Their studies showed that bone marrow-derived stem cells could still differentiate chondrogenically while retaining intracellular iron oxide, thereby permitting MRI detection within 3D collagen type I hydrogel constructs.…”

Section: Discussionmentioning

confidence: 99%

“…There has been controversy regarding the effect of SPIO labeling on chondrogenesis in MSCs, 36,44,48,49 although we have demonstrated here that cells already possessing the chondrocyte phenotype can be successfully labeled with SPIO complexes without promoting phenotypic instability. Our results indicate that labeled chondrocytes may be successfully incorporated into cartilage tissue engineering systems.…”

mentioning

confidence: 95%

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Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Ramaswamy

Greco

Uluer

et al. 2009

Tissue Engineering Part A

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 95%

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Ramaswamy

Greco

Uluer

et al. 2009

Tissue Engineering Part A

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“…[39][40][41][42][43][44] Earlier produced as VSOP-C184 (Ferropharm, Teltow, Germany), 45 these have been used for MSC labeling and found to have a detection limit of 40,000 cells in an 11.7 T MRI setting. 46 These NPs are no longer available. In the past decade, VSOP have been further developed in our laboratory for magnetic resonance angiography and MRI of atherosclerotic plaques.…”

Section: Introductionmentioning

confidence: 99%

Labeling of mesenchymal stem cells for MRI with single-cell sensitivity

Schellenberger¹,

Kratz²,

Farr³

et al. 2016

IJN

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Sensitive cell detection by magnetic resonance imaging (MRI) is an important tool for the development of cell therapies. However, clinically approved contrast agents that allow single-cell detection are currently not available. Therefore, we compared very small iron oxide nanoparticles (VSOP) and new multicore carboxymethyl dextran-coated iron oxide nanoparticles (multicore particles, MCP) designed by our department for magnetic particle imaging (MPI) with discontinued Resovist ® regarding their suitability for detection of single mesenchymal stem cells (MSC) by MRI. We achieved an average intracellular nanoparticle (NP) load of >10 pg Fe per cell without the use of transfection agents. NP loading did not lead to significantly different results in proliferation, colony formation, and multilineage in vitro differentiation assays in comparison to controls. MRI allowed single-cell detection using VSOP, MCP, and Resovist ® in conjunction with high-resolution T2*-weighted imaging at 7 T with postprocessing of phase images in agarose cell phantoms and in vivo after delivery of 2,000 NP-labeled MSC into mouse brains via the left carotid artery. With optimized labeling conditions, a detection rate of ~45% was achieved; however, the experiments were limited by nonhomogeneous NP loading of the MSC population. Attempts should be made to achieve better cell separation for homogeneous NP loading and to thus improve NP-uptake-dependent biocompatibility studies and cell detection by MRI and future MPI. Additionally, using a 7 T MR imager equipped with a cryocoil resulted in approximately two times higher detection. In conclusion, we established labeling conditions for new high-relaxivity MCP, VSOP, and Resovist ® for improved MRI of MSC with single-cell sensitivity.

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“…Some recent approaches to quantify cell distribution in tissue-engineered scaffolds include micro-computed tomography and magnetic resonance imaging of magnetic iron oxide nanoparticles entrapped in seeded cells. 33,34 Although these and other studies have investigated the distribution of cells within constructs, the need still exists for a quick and economical method that allows visualization of the three-dimensional (3D) distribution of cells while also allowing quantification of distribution and infiltration in the scaffold.…”

Section: Introductionmentioning

confidence: 99%

Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds

Thevenot

Nair

Dey

et al. 2008

Tissue Engineering Part C: Methods

149

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Effective cell seeding throughout the tissue scaffold often determines the success of tissue-engineering products, although most current methods focus on determining the total number, not the distribution, of the cells associated with tissue-engineering constructs. The purpose of this investigation was to establish a quick, convenient, and efficient method to quantify cell survival, distribution, and infiltration into degradable scaffolds using a combination of fluorescence cell staining and cryosectioning techniques. After cell seeding and culture for different periods of time, seeded scaffolds were stained with a live cell dye and then cryosectioned. Cryosectioned scaffolds were then recompiled into a three-dimensional (3D) image to visualize cell behavior after seeding. To test the effectiveness of this imaging method, four common seeding methods, including static surface seeding, cell injection, orbital shaker seeding, and centrifuge seeding, were investigated for their seeding efficacy. Using this new method, we were able to visualize the benefits and drawbacks of each seeding method with regard to the cell behavior in 3D within the scaffolds. This method is likely to provide useful information to assist the development of novel materials or cell-seeding methods for producing full-thickness tissue grafts.

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Iron oxide labelling of human mesenchymal stem cells in collagen hydrogels for articular cartilage repair

Cited by 112 publications

References 45 publications

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Labeling of mesenchymal stem cells for MRI with single-cell sensitivity

Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds

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