Iron mediates Trichomonas vaginalis resistance to complement lysis

Alderete, John F.; Provenzano, Daniele; Lehker, Michael W.

doi:10.1006/mpat.1995.0049

Cited by 82 publications

(75 citation statements)

References 28 publications

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“…9D), implying that such vesicles are the sites that restrain Myb1 and possibly other Myb transcription factors before their release into the cytoplasm and importation into the nucleus. 4 TvCyP1 was most abundant inside hydrogenosomes, implying that TvCyP1 might assist in the proper folding of some hydrogenosomal proteins for regulating energy metabolism. These speculations can be tested in future studies.…”

Section: Discussionmentioning

confidence: 98%

“…This activity is dependent on PPI activity because higher levels of Myb1 and Myb3 were associated with V Myb from cells overexpressing TvCyP1(R63A). Intriguingly, TvCyP1 might also complex with Myb1 and Myb3 in P100, which is enriched in plasma membrane and small vesicles like endosomes 4 but devoid of the hydrogenosomes and V Myb in P15 (Fig. 6).…”

Section: Discussionmentioning

confidence: 99%

“…Antibody Production-Purified recombinant proteins TvCyP1, TvCyP2 (TVAG_062520), 4 glycosyltransferase (TvGT) (TVAG_ 258220), 4 and heat shock protein (Hsp) 70 (TvHsp70-1) (TVAG_044510) 4 (34) and a protein with seven ankyrin repeats (TvAnk-1) (TVAG_016370) 4 (34) were used for immunization in mice and rats following a standard protocol (35). Antisera were collected and purified by protein A affinity chromatography as described by the supplier (Sigma).…”

Section: Dna Transfection and Selection Of Stable Transfectants-mentioning

confidence: 99%

“…Curative chemotherapy is available, but transmission is difficult to control, and reports on clinical drug-resistant isolates have been increasing in recent years (2,3). Upon infection, the parasite trophozoites can breach the mucosal and epithelial barriers, lyse red blood cells, degrade complements and other humoral factors in host defense, and elicit chronic inflammation (1,4). The infection has also been linked to the elevated transmission of human immunodeficiency virus (HIV) and human papillomaviruses (1,3,5,6), making this overlooked pathogen a threat to public health.…”

mentioning

confidence: 99%

“…A similar structural entity in Myb1 may also mediate its own nuclear translocation. 4 Much remains to be learned about the cellular machinery mediating the nuclear importation of Myb proteins in T. vaginalis.…”

mentioning

confidence: 99%

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Regulation of Nuclear Translocation of the Myb1 Transcription Factor by TvCyclophilin 1 in the Protozoan Parasite Trichomonas vaginalis

Hsu

Chu

Wang

et al. 2014

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Dna Transfection and Selection Of Stable Transfectants-mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Regulation of Nuclear Translocation of the Myb1 Transcription Factor by TvCyclophilin 1 in the Protozoan Parasite Trichomonas vaginalis

Hsu

Chu

Wang

et al. 2014

Journal of Biological Chemistry

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A further proteomic study on the effect of iron in the human pathogen Trichomonas vaginalis

et al. 2007

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Iron is an essential element to support the growth and survival of Trichomonas vaginalis. It plays a critical role in the host-parasite interaction, and modulates the expression of virulence factors in this protozoan. In this work, parasites grown in iron-rich and iron-depleted media were analyzed by (i) light and scanning electron microscopy and (ii) 2-DE and MS. Withdrawal of iron from the culture medium resulted in dramatic changes in both the morphology and in the proteome pattern of T. vaginalis. Trophozoites underwent transformation from ellipsoid or amoeboid forms to rounded cells, whose flagella and axostyle were internalized. Forty-five proteins differentially expressed in parasites cultivated in the absence of iron were identified. In iron-depleted parasites, enzymes involved in energetic metabolism, proteolysis and hydrogenosomal iron-sulfur (Fe-S) proteins were down-regulated or even suppressed. Among up-regulated proteins, six isoforms of actin were detected. In addition, phosphoenolpyruvate carboxykinase, putative lactate dehydrogenase, and putative adenosine triphosphatase were also up-regulated or were exclusively observed in gels related to iron-depleted parasites. Our data demonstrate that iron has a pivotal role in the regulation of the morphological transformation of T. vaginalis and modulates the expression of both Fe-S and non-Fe-S proteins in the parasite.

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Immunoproteomics of the active degradome to identify biomarkers for Trichomonas vaginalis

et al. 2010

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Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potential biomarkers for trichomonosis. The proteases detected when T. vaginalis protein extracts are incubated without protease inhibitors, the trichomonad-active degradome, and the immunoproteome were obtained by using 2-DE, 2-D-zymograms, 2-D-Western blot (WB) assays with trichomonosis patient sera, and MS analysis. Forty-nine silver-stained spots were detected in the region of 200-21 kDa of parasite protease-resistant extracts. A similar proteolytic pattern was observed in the 2-D zymograms. Nine CPs were identified in the 30 kDa region (TvCP1, TvCP2, TvCP3, TvCP4, TvCP4-like, TvCP12, TvCPT, TvLEGU-1, and another legumain-like CP). The major reactive spots to T. vaginalis-positive patient sera by 2-D-WB corresponded to four papain-like (TvCP2, TvCP4, TvCP4-like, TvCPT), and one legumain-like (TvLEGU-1) CPs. The genes of TvCP4, TvCPT, and TvLEGU-1 were cloned, sequenced, and expressed in Escherichia coli. Purified recombinant CPs were recognized by culture-positive patient sera in 1-D-WB assays. These data show that some CPs could be potential biomarkers for serodiagnosis of trichomonosis.

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Iron mediates Trichomonas vaginalis resistance to complement lysis

Cited by 82 publications

References 28 publications

Regulation of Nuclear Translocation of the Myb1 Transcription Factor by TvCyclophilin 1 in the Protozoan Parasite Trichomonas vaginalis

Regulation of Nuclear Translocation of the Myb1 Transcription Factor by TvCyclophilin 1 in the Protozoan Parasite Trichomonas vaginalis

A further proteomic study on the effect of iron in the human pathogen Trichomonas vaginalis

Immunoproteomics of the active degradome to identify biomarkers for Trichomonas vaginalis

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