PLoS ONE volume 9, issue 6, Pe98399 2014 DOI: 10.1371/journal.pone.0098399 View full text
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Viola Paulus-Hock, Eric C. Cheung, Patricia Roxburgh, Karen H. Vousden, Andreas K. Hock

Abstract: Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible with high throughput systems, in contrast to traditional methods which are often labor-intensive, subjective to bias and do not allow further analysis using the same cells. Transformation in a single layer soft agar ass…

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