Ions and osmolality on post‐thaw sperm motility activation of the endangered
 Brycon insignis
 (Characiformes)

Viveiros, Ana Tereza de Mendonça; Motta, Naiara Cristina; Isaú, Ziara Aparecida; Almeida, Izabella L.G.; Leal, Marcelo C.

doi:10.1111/jai.13857

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…These results agree with Aramli & Nazari (2014), who reported a 47 % decrease in the sperm motility of the Persian sturgeon Acipenser persicus 60 min after thawing. Viveiros et al (2019) observed a significant reduction in the activation capacity of Brycon insignis sperm after 20-25 min post-thaw. They have also observed it in the salmon river Brycon orbignyanus Valenciennes and dorado Salminus brasiliensis Cuvier, just a few seconds after thawing.…”

Section: Discussionmentioning

confidence: 78%

“…Similarly, in the silver barb Barbodes gonionotus, sperm motility was significantly reduced in thawed semen 10 months after cryo-storage (Vuthiphandchai et al, 2015). Viveiros et al (2019) evaluated B. insignis semen cryopreserved for five years, reporting post-thaw motility above 70 % through the use of different activator solutions with a wide range of osmolalities (0-200 mOsm kg -1 ), including SB and SC, without finding significant differences in the post-thaw motility obtained by the use of these two substances.…”

Section: Discussionmentioning

confidence: 96%

“…However, most studies in characiforms show that the main mechanism of activation is hypoosmolality. Viveiros et al (2019) stated that hypoosmolality is a key factor in the process related to the activation of sperm motility. Similarly, they observed that glucose solutions free of Na + , K +, and Ca 2+ fully activated the post-thaw sperm motility of Brycon insignis Steindachner, in a similar way to those observed after activation with NaCl, sodium citrate, and KCl, concluding that the osmolality of the activating solution instead of the presence of ions is the trigger for the activation of sperm motility.…”

Section: Discussionmentioning

confidence: 99%

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CRYOPRESERVED SEMEN OF Piaractus orinoquensis (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

Medina-Robles,

Suárez-Martínez,

Baldisserotto

et al. 2023

Acta biol. Colomb.

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Piaractus orinoquensis is a native species from the Colombian Orinoquia and several studies have contributed to the standardization of protocols for seminal cryopreservation of this species. The main goal of this study was to evaluate the sperm motility, duration of sperm motility (DM), pH, sperm membrane integrity (SMI) and fertility of semen cryopreserved for seven years and subjected to different post-thaw storage times (PST). Semen from five males (3.04 ± 0.3 kg) was diluted 1:4 (semen:diluent) with 10 % dimethylsulfoxide, 5.5 % glucose and 12 % egg yolk in 4 mL macrotubes and frozen in liquid nitrogen. Four PST were evaluated: 0, 15, 45 and 60 min. The sperm cells were activated with 1 % NaHCO3 (SB) and 0.9 % NaCl (SC) to determine sperm motility, DM, fertility rate, SMI and pH through PST. Significative motility reduction (p < .05) was observed through the PST and between the PST of 0 and 60 min for DM, both for treatments activated with SB and with SC. The SMI had a significant reduction (p < .05) after 60 min of post-thaw storage and the pH did not vary during PST. The fertility rate decreased significantly (p < .05) between time 0 and 60 min of PST. PST affects the seminal quality and fertility of P. orinoquensis, the best fertilization results were obtained by activating post-thawing sperm motility with 1 % SB. The long-term cryopreserved semen (seven years) of the species maintains its fertilization capacity with values like those obtained with fresh sperm.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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CRYOPRESERVED SEMEN OF Piaractus orinoquensis (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

Medina-Robles,

Suárez-Martínez,

Baldisserotto

et al. 2023

Acta biol. Colomb.

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“…The positive correlation of Ca 2+ concentration in seminal plasma with ROS levels, and the negative correlation between this ion and post‐thaw sperm motility rate indicate that the interaction of these agents disturbed spermatozoa metabolism. The negative effects of Ca 2+ on sperm motility have been demonstrated in characiforms and cyprinids (França et al, 2020; Khara et al, 2014; Viveiros et al, 2019). The freezing and thawing process disturb the mitochondrial oxidative metabolism, leading to enhanced ROS generation and dysregulation of mitochondrial Ca 2+ homeostasis, which in turn stimulates an increase in mitochondrial oxidative metabolism, resulting in mitochondrial dysfunction, impairment of ATP synthesis (Brookes et al, 2004) and ultimately loss of post‐thaw sperm motility (Figueroa et al, 2016, 2019).…”

Section: Discussionmentioning

confidence: 99%

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

et al. 2021

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The aim of this study was to determine the effect of seasonality on post‐thaw sperm quality in Prochilodus lineatus. Sperm from 43 males was cryopreserved throughout the reproductive season (November–March). Fresh sperm quality, concentration, volume, seminal plasma pH, osmolality and ion concentration were analysed. Post‐thaw sperm was analysed for its motility rate, velocities, beat‐cross frequency, cell morphology, oxidative stress and fertilization capacity. Prochilodus lineatus fresh sperm yielded high‐quality during the reproductive period (motility >80%). Post‐thaw sperm quality changed throughout the season, with higher motility (63.2%–72.3%) and curvilinear velocity (VCL) (55.9–59.2 µm/s) from December to March. Sperm concentration was negatively correlated with post‐thaw sperm motility and VCL, and positively correlated with seminal plasma Ca2+ concentration, catalase activity (CAT) and reactive oxygen species (ROS). Seminal plasma Ca2+ was positively correlated with ROS and negatively correlated with post‐thaw sperm motility. Post‐thaw sperm CAT and fertility correlated positively. Although post‐thaw sperm quality was reduced in November, the increased CAT was efficient in maintaining its fertilization capacity. In order to face seasonal influence, the optimal period to cryopreserve P. lineatus spermatozoa is from December to March, when sperm exhibits characteristics which make spermatozoa more prone to resist cryodamage.

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“…Therefore, NaCl solution at a concentration of 325 mOsm kg -1 and a pH of 7.6 guarantees the immobility of sperm by promoting conditions similar to seminal plasma. In addition, the presence of NaCl at the time of activation potentiates the sperm kinetic parameters (Viveiros et al, 2019), increasing the chances of fertilization.…”

Section: Introductionmentioning

confidence: 99%

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

de Souza França

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Fish sperm cryopreservation is a technique that can increase the reproduction in captive efficiency of freshwater and marine fishes. The technique is applied in environmental conservation programs and aquaculture. Over the last few decades, protocols for sperm cryopreservation from many fishes have been established. However, the researchers' main focus was successfully freezing and thawing sperm, neglecting the period in which the gametes remain in contact with the cryoprotective solution until fertilization. Exposure of sperm to cryoprotectant solutions after thawing can be harmful to the quality of the gametes since they can be toxic.The vast majority of the established protocols use ultra-resistant plastic containers to store sperm during the cryopreservation process. These containers usually are not reused, generating highly polluting waste for the environment. Furthermore, in some countries, the containers usually used are sold by a few industries, which makes acquisition difficult and increases the product's price. Thus, the main objective of the thesis was to create and test low-cost methodologies that enhance the use of fish post-thaw sperm and make the sperm cryopreservation process more environmentally friendly.The experiments in the Chapters 1 and 2 were developed in Brazil. We used the South American silver catfish Rhamdia quelen, a species considered an experimental model for native South American fishes. In vitro fertilization and sperm cryopreservation protocols for the species were already established; thus, we based our work on these protocols to apply innovations.In Chapter 1, we tested the use of post-thawing dilution to reduce the toxicity of the cryoprotectant solution. This technique is commonly used in mammalian sperm cryopreservation protocols but has never before been applied to post-thaw sperm of South American fishes. South American silver catfish post-thaw sperm samples were diluted in a saline extender (1.1% NaCl -325 mOsm kg -1 ; pH 7.6). The post-thaw sperm diluted samples showed higher velocities, straightness, progression, and flagellar beat frequency than SUMMARY 2 the cells of undiluted samples (control). The post-thawing dilution also provided higher fertilization and hatching rates than the control group. Thus, the post-thawing sperm dilution proved to be a simple, cheap, and efficient methodology that should be included in the silver catfish sperm cryopreservation protocol.

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Ions and osmolality on post‐thaw sperm motility activation of the endangered Brycon insignis (Characiformes)

Cited by 6 publications

References 29 publications

CRYOPRESERVED SEMEN OF <i>Piaractus orinoquensis</i> (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

CRYOPRESERVED SEMEN OF <i>Piaractus orinoquensis</i> (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

Sperm cryopreservation of Prochilodus lineatus throughout the same reproductive season

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

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