Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.12.078 View full text
Lin-Ling Yu, Xiao-Yan Dong, Yan Sun

Abstract: We have previously found that addition of charged particles in a refolding solution can greatly increase the refolding yield of like-charged proteins. Herein, porous anion exchangers of different charged group densities, ligand chemistries, pore sizes and particle sizes were prepared with Sepharose FF gel for studying their effects on the oxidative refolding of like-charged lysozyme. We found that charge density had significant contribution to the enhancing effects on lysozyme refolding. At low resin concentra…

expand abstract