Ion chromatographic method for the analysis of sulfate in complex carbohydrates

Colloidal Cutin-Like Substances Cross-Linked to Siderophore Decomposition Products Mobilizing Plutonium from Contaminated Soils

“…Characterization included the spectrophotometric analysis of total carbohydrates, uronic acids , proteins , and phosphate ; elemental analysis of organic C and N by Perkin-Elmer CHNS 2400 analyzer ; determination of Fe, Al, and Mn by Perkin-Elmer 5100 Zeeman GFAAS ; and measurement of sulfate by ion chromatography (Dionex, Houston, TX) with an IonPac AG4A anion guard and analytical columns, after an acid hydrolysis step . For the IEF extract, the molecular weight was determined by using a Tosoh Biosciences G4000 PWxl guard and analytical size exclusion columns coupled with a Waters HPLC system.…”

Colloidal Cutin-Like Substances Cross-Linked to Siderophore Decomposition Products Mobilizing Plutonium from Contaminated Soils

“…Characterization included the spectrophotometric analysis of total carbohydrates, uronic acids , proteins , and phosphate ; elemental analysis of organic C and N by Perkin-Elmer CHNS 2400 analyzer ; determination of Fe, Al, and Mn by Perkin-Elmer 5100 Zeeman GFAAS ; and measurement of sulfate by ion chromatography (Dionex, Houston, TX) with an IonPac AG4A anion guard and analytical columns, after an acid hydrolysis step . For the IEF extract, the molecular weight was determined by using a Tosoh Biosciences G4000 PWxl guard and analytical size exclusion columns coupled with a Waters HPLC system.…”

Determination of free and total sulfate and phosphate in glycosaminoglycans by column-switching high-performance size-exclusion and ion chromatography and single-column ion chromatography

“…Grotjan et al determined total and free sulfate contents in heparin by IC. 26 However, direct injection of polysaccharide samples into the anion-exchange column would affect the anion separation, dramatically reducing the life of the columns. Coupling high-performance size-exclusion chromatography (HPSEC) to IC for the determination of contaminant anions is particularly suitable, since the use of a column-switching system allows the transfer of GAG-free fractions containing the anionic analytes from the HPSEC to the IC column.…”

Colloidal Cutin-Like Substances Cross-Linked to Siderophore Decomposition Products Mobilizing Plutonium from Contaminated Soils

“…Characterization included the spectrophotometric analysis of total carbohydrates, uronic acids , proteins , and phosphate ; elemental analysis of organic C and N by Perkin-Elmer CHNS 2400 analyzer ; determination of Fe, Al, and Mn by Perkin-Elmer 5100 Zeeman GFAAS ; and measurement of sulfate by ion chromatography (Dionex, Houston, TX) with an IonPac AG4A anion guard and analytical columns, after an acid hydrolysis step . For the IEF extract, the molecular weight was determined by using a Tosoh Biosciences G4000 PWxl guard and analytical size exclusion columns coupled with a Waters HPLC system.…”

Determination of free and total sulfate and phosphate in glycosaminoglycans by column-switching high-performance size-exclusion and ion chromatography and single-column ion chromatography

“…Grotjan et al determined total and free sulfate contents in heparin by IC. 26 However, direct injection of polysaccharide samples into the anion-exchange column would affect the anion separation, dramatically reducing the life of the columns. Coupling high-performance size-exclusion chromatography (HPSEC) to IC for the determination of contaminant anions is particularly suitable, since the use of a column-switching system allows the transfer of GAG-free fractions containing the anionic analytes from the HPSEC to the IC column.…”

Colloidal Cutin-Like Substances Cross-Linked to Siderophore Decomposition Products Mobilizing Plutonium from Contaminated Soils

“…Characterization included the spectrophotometric analysis of total carbohydrates, uronic acids , proteins , and phosphate ; elemental analysis of organic C and N by Perkin-Elmer CHNS 2400 analyzer ; determination of Fe, Al, and Mn by Perkin-Elmer 5100 Zeeman GFAAS ; and measurement of sulfate by ion chromatography (Dionex, Houston, TX) with an IonPac AG4A anion guard and analytical columns, after an acid hydrolysis step . For the IEF extract, the molecular weight was determined by using a Tosoh Biosciences G4000 PWxl guard and analytical size exclusion columns coupled with a Waters HPLC system.…”

Determination of free and total sulfate and phosphate in glycosaminoglycans by column-switching high-performance size-exclusion and ion chromatography and single-column ion chromatography

“…Grotjan et al determined total and free sulfate contents in heparin by IC. 26 However, direct injection of polysaccharide samples into the anion-exchange column would affect the anion separation, dramatically reducing the life of the columns. Coupling high-performance size-exclusion chromatography (HPSEC) to IC for the determination of contaminant anions is particularly suitable, since the use of a column-switching system allows the transfer of GAG-free fractions containing the anionic analytes from the HPSEC to the IC column.…”