Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis

Wang, Yuan-Li; Li, Qing; Xie, Jing; Zhu, Ming; Sun, Wen-Juan; Wang, Qun

doi:10.1016/j.gene.2013.11.099

Cited by 12 publications

(7 citation statements)

References 42 publications

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“…They also found that interfering with cul-4 expression in C. elegans causes DNA replication defects (due to improper Cdt1 regulation as described in detail above) as well as premature meiosis activity and sperm formation in germ cell lineages. Similar findings were reported last year by a group researching Cul4 expression during Chinese mitten crab development (Wang et al, 2014b). Despite the functional redundancy of CUL4A and CUL4B, their patterns of expression often differ spatially and temporally — highlighting key, non-overlapping roles for each protein in mature and developing tissues.…”

Section: Cellular and Developmental Functions Of Crl4 Ligasessupporting

confidence: 90%

Distinct and overlapping functions of the cullin E3 ligase scaffolding proteins CUL4A and CUL4B

Hannah

Zhou

2015

Gene

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The cullin 4 subfamily of genes includes CUL4A and CUL4B, which share a mostly identical amino acid sequence aside from the elongated N-terminal region in CUL4B. Both act as scaffolding proteins for modular cullin RING ligase 4 (CRL4) complexes which promote the ubiquitination of a variety of substrates. CRL4 function is vital to cells as loss of both genes or their shared substrate adaptor protein DDB1 halts proliferation and eventually leads to cell death. Due to their high structural similarity, CUL4A and CUL4B share a substantial overlap in function. However, in some cases, differences in subcellular localization, spatiotemporal expression patterns and stress-inducibility preclude functional compensation. In this review, we highlight the most essential functions of the CUL4 genes in: DNA repair and replication, chromatin-remodeling, cell cycle regulation, embryogenesis, hematopoiesis and spermatogenesis. CUL4 genes are also clinically relevant as dysregulation can contribute to the onset of cancer and CRL4 complexes are often hijacked by certain viruses to promote viral replication and survival. Also, mutations in CUL4B have been implicated in a subset of patients suffering from syndromic X-linked intellectual disability (AKA mental retardation). Interestingly, the antitumor effects of immunomodulatory drugs are caused by their binding to the CRL4CRBN complex and re-directing the E3 ligase towards the Ikaros transcription factors IKZF1 and IKZF3. Because of their influence over key cellular functions and relevance to human disease, CRL4s are considered promising targets for therapeutic intervention.

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Section: Cellular and Developmental Functions Of Crl4 Ligasessupporting

confidence: 90%

Distinct and overlapping functions of the cullin E3 ligase scaffolding proteins CUL4A and CUL4B

Hannah

Zhou

2015

Gene

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“…Moreover, DDB1 is also required for the maintenance of spermatogonial stem cells (SSCs) (Yu et al, 2016). Previously, we reported that Es-Cul4 might play an important role in regulating spermatogenesis (Wang et al, 2014). Initially, we identified Es-DDB1 and Es-CUL4 as interacting proteins, and the expression pattern of Es-Cul4 in spermatogenesis was similar to that of Es-DDB1.…”

Section: Discussionmentioning

confidence: 95%

“…Testes undergo five developmental stages during spermatogenesis (spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and spermatozoa), whose morphological and histological structures can be differentiated (Du, 1988b;Wang et al, 2014). We used immunofluorescence analysis to confirm the subcellular localization of DDB1 in each of the five stages of spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

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A core component of the CUL4 ubiquitin ligase complexes, DDB1, regulates spermatogenesis in the Chinese mitten crab, Eriocheir sinensis

Fang

Wang

et al. 2017

Gene

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“…Eriocheir sinensis is a commercially aquatic crustacean widely bred in China and has a classic decondensed nucleus in their spermatozoon. During the recent three decades, this species has been used as a model system for studying the crustacean spermatogenesis (Du et al 1987a;Yu et al 2009;Mao et al 2012;Hou and Yang 2013;Wang et al 2014;He et al 2015;Wang et al 2015;Wu et al 2015). Spermatogenesis in this crab also includes the stages of spermatogonia, spermatocytes and spermiogenesis as in other animals (Du et al 1987a;Wu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

H3K9ac involved in the decondensation of spermatozoal nuclei during spermatogenesis in Chinese mitten crab Eriocheir sinensis

Kang

et al. 2016

Cytotechnology

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As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding of the nuclear decondensation of the spermatozoa in E. sinensis. Using specific antibodies, we followed the structural distribution and acetylated lysine 9 of the histone H3 during spermatogenesis, especially spermiogenesis, of E. sinensis. Various spermary samples at different developmental stages were used for histological immunofluorescence and ultrastructural immunocytochemistry. Our results demonstrate a wide distribution of the histone H3 and H3K9ac during spermatogenesis, including spermatogonia, spermatocytes, spermatids, and immature and mature spermatozoa except for absence of H3K9ac in the secondary spermatocytes. Especially during the initial stage of nuclear decondensation, histone H3 lysine 9 was acetylated and then an amount of H3K9ac was removed from within to outside of the nuclei of late spermatids. The portion of remaining H3K9ac was gradually transferred from the nuclei during the stages of spermatozoa maturation. Our findings suggest both the acetylation of histone H3 lysine 9 and the remain of H3K9ac to contribute to the nuclear decondensation in spermatozoa of E. sinensis.

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Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis

Cited by 12 publications

References 42 publications

Distinct and overlapping functions of the cullin E3 ligase scaffolding proteins CUL4A and CUL4B

Distinct and overlapping functions of the cullin E3 ligase scaffolding proteins CUL4A and CUL4B

A core component of the CUL4 ubiquitin ligase complexes, DDB1, regulates spermatogenesis in the Chinese mitten crab, Eriocheir sinensis

H3K9ac involved in the decondensation of spermatozoal nuclei during spermatogenesis in Chinese mitten crab Eriocheir sinensis

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