Involvement of the nadA gene in formation of G-group aflatoxins in Aspergillus parasiticus

The production of aflatoxin B1 or G1 by Aspergillus parasiticus at various combinations of temperature and water activity is related to the ratio of aflS to aflR expression

“…As a modulator, aflS may influence the activity of aflR. In the case of A. parasiticus, this modulation may shift the affinity of aflR towards the G-group-specific genes, ordA, cypA and nadA (Cai et al 2008) and activate their expression under these conditions. This differential production of aflatoxin B 1 and G 1 surely has an ecological function and may be an adaptation of A. parasiticus to a similar but slightly different habitat compared to A. flavus (Magan and Aldred 2007a, b).…”

Aflatoxins: Mechanisms of Inhibition by Antagonistic Plants and Microorganisms

“…Most recently, Zeng et al (2011) confirmed in A. parasiticus that HOMST was converted to AFG 1 and AFB 1 . Cai et al, 2008 showed that the nadA gene previously assigned to the adjacent sugar utilization gene cluster instead is required for the formation of AFG 1 and AFG 2 . The nadA knockout mutants of A. parasiticus accumulate a new 360 Da precursor, NADA.…”

Identification of three mutant loci conferring carboxin-resistance and development of a novel transformation system in Aspergillus oryzae

“…To transform the pPTRI (Takara, Japan) plasmid that contains the pyrithiamine-resistant gene (ptrA), the plasmid was linearized with HindIII. Protoplast preparation and transformation were carried out as described previously (Cai et al, 2008;Wen et al, 2005). The transformation selected by the pyrithiamine-resistance was performed according to the method described previously (Wen et al, 2005).…”