Involvement of intracellular signaling cascades in inflammatory responses in human intestinal epithelial cells following Vibrio cholerae infection

Bandyopadhaya, Arunava; Das, Debarup; Chaudhuri, Keya

doi:10.1016/j.molimm.2008.11.003

Cited by 18 publications

(17 citation statements)

References 49 publications

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“…For example, they can be used to evaluate the effects of growth or maturation factors on the expression of membrane receptors and antigens, enzymatic activities, transport and secretory functions (37), to study drug metabolism, and to investigate carcinogenic factors and agonists (38). Furthermore, they contribute to growth and functional alterations of the colonic epithelium in intestinal cancers, IBD and other diseases and to the understanding of the mechanisms of interactions of intestinal pathogens with the mucosal surface (39) and the intracellular signaling transduction (40).…”

Section: Discussionmentioning

confidence: 99%

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

Liu

Zhang

Zhou

et al. 2010

Braz J Med Biol Res

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Epithelium, a highly dynamic system, plays a key role in the homeostasis of the intestine. However, thus far a human intestinal epithelial cell line has not been established in many countries. Fetal tissue was selected to generate viable cell cultures for its sterile condition, effective generation, and differentiated character. The purpose of the present study was to culture human intestinal epithelial cells by a relatively simple method. Thermolysin was added to improve the yield of epithelial cells, while endothelin-3 was added to stimulate their growth. By adding endothelin-3, the achievement ratio (viable cell cultures/total cultures) was enhanced to 60% of a total of 10 cultures (initiated from 8 distinct fetal small intestines), allowing the generation of viable epithelial cell cultures. Western blot, real-time PCR and immunofluorescent staining showed that cytokeratins 8, 18 and mouse intestinal mucosa-1/39 had high expression levels in human intestinal epithelial cells. Differentiated markers such as sucrase-isomaltase, aminopeptidase N and dipeptidylpeptidase IV also showed high expression levels in human intestinal epithelial cells. Differentiated human intestinal epithelial cells, with the expression of surface markers (cytokeratins 8, 18 and mouse intestinal mucosa-1/39) and secretion of cytokines (sucrase-isomaltase, aminopeptidase N and dipeptidylpeptidase IV), may be cultured by the thermolysin and endothelin-3 method and maintained for at least 20 passages. This is relatively simple, requiring no sophisticated techniques or instruments, and may have a number of varied applications.

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Section: Discussionmentioning

confidence: 99%

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

Liu

Zhang

Zhou

et al. 2010

Braz J Med Biol Res

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“…Proinflammatory cytokine secretion and inflammatory mediator-induced cytokine expression are dependent on PLC in human epithelial and synovial fibroblasts cell, suggesting that activation of PLC is required in signal transduction events, leading to inflammatory response (15,52). Several studies suggest that PLC␥1 regulates PLD activity in intracellular signal transduction (42,53,54).…”

Section: Discussionmentioning

confidence: 99%

“…The differences in the localization of these PLDs can provide important clues, suggesting their specific roles in various situations and cell types (13). PLD and phospholipase C (PLC) ␥ play an important regulatory role in cell immune response (14,15). The activation of PLC␥1 results in the hydrolysis of phosphatidylinositol 4,5-bisphosphates (PIP 2 ) to generate inositol 1,4,5-trisphosphates (IP 3 ) and diacylglycerol (16,17).…”

mentioning

confidence: 99%

House Dust Mite Allergen Der f 2-induced Phospholipase D1 Activation Is Critical for the Production of Interleukin-13 through Activating Transcription Factor-2 Activation in Human Bronchial Epithelial Cells

Park

Cho

et al. 2009

Journal of Biological Chemistry

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The purpose of this study was to identify the role of phospholipase D1 (PLD1) in Der f 2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f 2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f 2-induced IL-13 expression and production. Treatment of Der f 2 activated the phospholipase C␥ (PLC␥)/protein kinase C␣ (PKC␣)/p38 MAPK pathway. Der f 2-induced PLD activation was attenuated by PLC␥ inhibitors (U73122 and PAO), PKC␣ inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLC␥, PKC␣, and p38 MAPK act as upstream activators of PLD in Der f 2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f 2 were also blocked by inhibition of PLC␥, PKC␣, or p38 MAPK, indicating that IL-13 expression and production are related to a PLC␥/PKC␣/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f 2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f 2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f 2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f 2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.

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“…studies have reported that rapid MAPK activation occurs within intestinal epithelial cells in response to V. cholerae stimulation (29). Therefore, we evaluated whether V. cholerae O395 OMV could induce NOD1-dependent MAPK phosphorylation.…”

Section: Cholerae Omvs Activate Mapk and Nf-b Pathways In Epithelimentioning

confidence: 98%

Vibrio cholerae O395 Outer Membrane Vesicles Modulate Intestinal Epithelial Cells in a NOD1 Protein-dependent Manner and Induce Dendritic Cell-mediated Th2/Th17 Cell Responses

Chatterjee¹,

Chaudhuri

2013

Journal of Biological Chemistry

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Background: Vibrio cholerae, the etiologic agent of cholera, secretes outer membrane vesicles (OMVs) that are internalized into host cells. Results: OMVs activate an inflammatory response in intestinal epithelial cells (ECs) via a NOD1-dependent pathway that activates dendritic cells (DCs) and promotes T cell polarization toward Th2/Th17 responses. Conclusion: OMVs stimulate EC-DC cross-talk in generating an inflammatory response. Significance: Findings are important for the development of efficient vaccine strategies with OMVs.

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Involvement of intracellular signaling cascades in inflammatory responses in human intestinal epithelial cells following Vibrio cholerae infection

Cited by 18 publications

References 49 publications

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

House Dust Mite Allergen Der f 2-induced Phospholipase D1 Activation Is Critical for the Production of Interleukin-13 through Activating Transcription Factor-2 Activation in Human Bronchial Epithelial Cells

Vibrio cholerae O395 Outer Membrane Vesicles Modulate Intestinal Epithelial Cells in a NOD1 Protein-dependent Manner and Induce Dendritic Cell-mediated Th2/Th17 Cell Responses

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